A5040070457- Ubiquitin and proteasome pathways
- Molecular Sensors and Ion Detection
- Sulfur Compounds in Biology
- Intensive Care Unit Cognitive Disorders
- Perovskite Materials and Applications
- Protein Degradation and Inhibitors
- Anesthesia and Sedative Agents
- Head and Neck Cancer Studies
- Esophageal and GI Pathology
- Cardiac, Anesthesia and Surgical Outcomes
- Esophageal Cancer Research and Treatment
- Obstructive Sleep Apnea Research
- Tracheal and airway disorders
- Ferroptosis and cancer prognosis
- Quantum Dots Synthesis And Properties
- Cancer-related gene regulation
- Nitric Oxide and Endothelin Effects
- Radiomics and Machine Learning in Medical Imaging
- Lipid metabolism and biosynthesis
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
- Chalcogenide Semiconductor Thin Films
- Conducting polymers and applications
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
Georgia State University
2017-2024
Hangzhou Dianzi University
2023-2024
First Affiliated Hospital of GuangXi Medical University
2004-2022
Guangxi Medical University
2004-2022
Dalian Medical University
2014-2017
First Affiliated Hospital of Dalian Medical University
2016-2017
National Natural Science Foundation of China
2016
City University of Hong Kong
2014-2015
City University of Hong Kong, Shenzhen Research Institute
2015
Kowloon Hospital
2014-2015
A simple and versatile probe was discovered for discriminating between different thiol species with the use of CTAB (cetyl trimethylammonium bromide).
A new fluorescent probe installed with dual-reactive and dual-quenching groups was rationally designed for highly selective sensitive sensing of biothiols. The sensitivity the toward thiols significantly improved by effects. Furthermore selectivity also greatly enhanced installation groups.
HNO has recently been found to possess distinct biological functions from NO. Studying the of calls for development sensitive and selective fluorescent probes. Herein, we designed synthesized a FRET-based ratiometric probe detect in living cells. Our studies revealed that is capable detecting rapid manner under physiological conditions. In bioimaging studies, displayed clear color change blue green when treated with HNO.
Abstract While extensive investigations have been devoted to the study of genetic pathways related fatty liver diseases, much less is known about epigenetic mechanisms underlying these disorders. DNA methylation an link between environmental factors (e.g., diets) and complex diseases non‐alcoholic disease). Here, it aimed role in regulation hepatic lipid metabolism. A dynamic change methylome high‐fat diet (HFD)‐fed mice discovered, including a marked increase at promoter Beta‐klotho ( Klb)...
Engineering the ubiquitin transfer cascades by phage display enables an efficient way to profile E3 substrates.
The highly infectious coronavirus SARS-CoV-2 relies on the viral main protease (Mpro, also known as 3CLpro or Nsp5) to proteolytically process polyproteins encoded by genome for release of functional units in host cells initiate replication. Mpro interacts with proteins innate immune pathways, such IRF3 and STAT1, suppress their activities facilitate virus survival proliferation. To identify mechanism regulating Mpro, we screened various classes E3 ubiquitin ligases found that Parkin...
Abstract E3 ubiquitin (UB) ligases are the ending modules of E1–E2-E3 cascades that transfer UB to cellular proteins and regulate their biological functions. Identifying substrates an holds key elucidate its role in cell regulation. Here, we construct orthogonal (OUT) cascade identify E6AP, a HECT also known as Ube3a is implicated cancer neurodevelopmental disorders. We use yeast surface display engineer E6AP exclusively affinity-tagged variant (xUB) substrate proteins. Proteomic...
Abstract Nitroxyl (HNO), the reduced and protonated form of nitric oxide (NO), has recently been identified as an interesting important signaling molecule in biological systems. However, research on its biosynthesis bioactivities are hampered by lack versatile HNO detection methods applicable to living cells. In this report, two new near-infrared (NIR) probes were designed synthesized for imaging One was found display high sensitivity towards HNO, with up 67-fold fluorescence increment after...
Manipulating the activities of E3 ubiquitin ligases with chemical ligands holds promise for correcting malfunctions and repurposing E3s induced protein degradation in cell. Herein, we report an alternative strategy to proteolysis-targeting chimeras (PROTACs) molecular glues induce by constructing screening a γ-AA peptide library cyclic peptidomimetics binding HECT domain E6AP, ubiquitinating p53 coerced human papillomavirus regulating pathways implicated neurodevelopmental disorders such as...
Nonalcoholic fatty liver disease (NAFLD) is substantiated by the reprogramming of metabolic pathways that disrupts homeostasis lipid and glucose metabolism thus promotes progression disease. The associated with NAFLD are regulated at different levels from gene transcription to various post-translational modifications including ubiquitination. Here, we used a novel orthogonal ubiquitin transfer platform identify pyruvate dehydrogenase A1 (PDHA1) acetyl-CoA acetyltransferase 1 (ACAT1), two...
Due to the unique characteristics of solution preparation and rapid crystal growth, perovskite solar cells may exhibit various kinds defects that ultimately impact their photovoltaic performance stability. Here, a multifunctional additive, potassium trifluoromethanesulfonate (KTFS), is introduced into lead iodide precursor solution. anions, positive work function shift (more p type) observed, which benefits extraction holes. The cation can occupy lattice gaps effectively inhibit ion...
Background:Postoperative delirium (POD) is a common complication in the elderly.This retrospective study investigated effect of intraoperative hemodynamics on incidence POD elderly patients after major surgery to explore ways reduce POD. Material/Methods:Based POD, (81±6 y) were assigned (n=137) or non-POD group (n=343) elective with total intravenous anesthesia.POD was diagnosed based guidelines Diagnostic and Statistical Manual Mental Disorders, Fourth Edition (DSM-IV), using confusion...
Glycosyltransferase OGT catalyzes the conjugation of O-linked β-D-N-acetylglucosamine (O-GlcNAc) to Ser and Thr residues cellular proteins regulates many key processes in cell. Here, we report identification as a ubiquitination target HECT-type E3 ubiquitin (UB) ligase E6AP, whose overexpression HEK293 cells would induce degradation OGT. We also found that expression E6AP HeLa with endogenous E6 protein human papillomavirus (HPV) accelerate by proteasome suppress O-GlcNAc modification...
Abstract Target validation is key to the development of protein degrading molecules such as proteolysis‐targeting chimeras (PROTACs) identify cellular proteins amenable for induced degradation by ubiquitin‐proteasome system (UPS). Previously HaloPROTAC was developed screen targets PROTACs linking chlorohexyl group with ligands E3 ubiquitin ligases VHL and cIAP1 recruit target fused HaloTag E3‐catalyzed ubiquitination. Reported here are HaloPROTACs that engage cereblon (CRBN) ubiquitinate...
To evaluate intranasal administration of butorphanol on postoperative pain and early cognitive dysfunction in old patients undergoing H-uvulopalatopharyngoplasty (H-UPPP). A total 260 male (65 to 77 years old) with obstructive sleep apnea hypopnea syndrome scheduled for H-UPPP were divided randomly receive butorphanol, intravenous fentanyl, or saline (controls). The definition preemptive analgesia is that the tested drugs are given before anesthesia induction. Visual analog scale (VAS)...
Abstract Purpose Although conventional sonography is used widely for evaluation of the gastroesophageal junction, its use in cervical esophagus still limited. The aim this study was to assess demonstrate portion esophagus. Methods esophagi 60 cadavers and 435 healthy volunteers were examined sonographically. Among subjects 182 scanned with a transducer operating at 7.5 MHz, 183 10.0‐MHz transducer, 70 12.0‐MHz transducer. Sonographic layer patterns compared among groups. histologic analyses...
Objective The aims of this study were to describe the relationship between scanning planes and appearance upper airway on sonography demonstrate reliability reproducibility sonographic measurements airway. Methods Airway sonoanatomy was recognized by comparing images corresponding cadaver’s anatomical specimens. Systemic examination 267 healthy volunteers conducted obtain measurement lumen. studies in 40 volunteers. Result air-filled appeared as a bright heterogeneous hyperechoic line....
To evaluate the value of ultrasound-based radiomics in preoperative prediction type I and II epithelial ovarian cancer.A total 154 patients with cancer were enrolled retrospectively. There 102 unilateral lesions 52 bilateral among a 206 lesions. The data for randomly divided into training set (53 + 71 II) test (36 46 by random sampling. ITK-SNAP software was used to manually outline boundary tumor, that is, region interest, 4976 features extracted. quantitative expression values normalized...
Abstract High‐quality perovskite absorption layer is the fundamental basis for efficient and stable solar cells (PSCs). Due to ionic nature of components, plentiful charged defects suspension bonds remain on surface grains after continuous high‐temperature annealing. Here, complex initiated by introduction a multifunctional imidazolidinyl urea (IU) additive into PbI 2 precursor solution could serve as nucleation sites crystallization templates crystals optimize growth high‐quality films. By...
Postoperative cognitive dysfunction (POCD), common in elderly patients, is thought to be closely associated with intraoperative instability of hemodynamics and excessive excretion tumor necrosis factor-α (TNF-α). Methoxamine a blood-pressure increasing drug commonly used for maintaining hemodynamics. potentially promotes TNF-α expression, leading an increased risk POCD. This study aimed investigate the dose-dependent effect methoxamine on incidence early POCD blood level. single-center...