A5007189074- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- Photoreceptor and optogenetics research
- Cellular transport and secretion
- Alzheimer's disease research and treatments
- Neurogenesis and neuroplasticity mechanisms
- S100 Proteins and Annexins
- Cellular Mechanics and Interactions
- Neural dynamics and brain function
- Ion channel regulation and function
- Axon Guidance and Neuronal Signaling
- Parkinson's Disease Mechanisms and Treatments
- Nuclear Receptors and Signaling
- Thyroid Cancer Diagnosis and Treatment
- Nuclear Structure and Function
- Neurological disorders and treatments
- Cell Adhesion Molecules Research
- Cell Image Analysis Techniques
- Neurological Disease Mechanisms and Treatments
- Prion Diseases and Protein Misfolding
- Wnt/β-catenin signaling in development and cancer
- Synthesis of β-Lactam Compounds
- Congenital gastrointestinal and neural anomalies
- Clusterin in disease pathology
Bogomoletz Institute of Physiology
2011-2022
National Academy of Sciences of Ukraine
1999-2022
Universität Hamburg
2003-2015
University Medical Center Hamburg-Eppendorf
2011-2012
University of Hong Kong
2003
National Academy of Medical Sciences of Ukraine
1998
Institute of Geology Ufa Scientific Center
1990
The neural cell adhesion molecule (NCAM) regulates synapse formation and synaptic strength via mechanisms that have remained unknown. We show NCAM associates with the postsynaptic spectrin-based scaffold, cross-linking N-methyl-d-aspartate (NMDA) receptor Ca2+/calmodulin-dependent protein kinase II alpha (CaMKIIalpha) in a manner not firmly or directly linked to PSD95 alpha-actinin. Clustering of promotes detergent-insoluble complexes enriched proteins resembling densities. Disruption...
Neurotransmission is mediated by the exocytic release of neurotransmitters from readily releasable synaptic vesicles (SVs) at active zone. To sustain neurotransmission during periods elevated activity, release-ready need to be replenished reserve pool SVs. The SV-associated synapsins are crucial for maintaining this and regulate mobilization How replenishment SVs regulated which other factors cooperate with in process unknown. Here we identify endocytic multidomain scaffold protein...
L1, a neural cell adhesion molecule of the immunoglobulin superfamily, is involved in neuronal migration and differentiation axon outgrowth guidance. Mutations human mouse L1 gene result similarly severe neurological abnormalities. To dissociate functional roles adult brain from developmental abnormalities, we have generated mutant which inactivated by cre-recombinase under control calcium/calmodulin-dependent kinase II promoter. This (L1fy+) did not show overt morphological behavioral...
Abstract The cell adhesion molecule, CHL1, like its close homologue L1, is important for normal brain development and function. In this study, we analysed the functional role of CHL1 in synaptic transmission CA1 region hippocampus using juvenile CHL1‐deficient (CHL1–/–) wild‐type (CHL1+/+) mice. Inhibitory postsynaptic currents evoked pyramidal cells by minimal stimulation perisomatically projecting interneurons were increased CHL1–/– mice compared with littermates. Also, long‐term...
Relatively mild ischemic episode can initiate a chain of events resulting in delayed cell death and significant lesions the affected brain regions. We studied early synaptic modifications after brief ischemia modeled rats by transient vessels' occlusion vivo or oxygen–glucose deprivation vitro hippocampal CA1 pyramidal cells. Electron microscopic analysis excitatory spine synapses stratum radiatum revealed rapid increase postsynaptic density (PSD) thickness length, as well formation concave...
In humans, deletions/mutations in the CHL1/CALL gene are associated with mental retardation and schizophrenia. Juvenile CHL1-deficient (CHL1(-/-) ) mice have been shown to display abnormally high numbers of parvalbumin-expressing (PV(+) hippocampal interneurons and, as adults, behavioral traits observed neuropsychiatric disorders. Here, we addressed question whether inhibitory synaptic plasticity CHL1(-/-) mouse affected during brain maturation adulthood. We found that hippocampal, but not...
Abstract Accumulating evidence suggests that extracellular matrix (ECM) molecules play important roles in formation of synapses. Our previous electrophysiologic study mice deficient the glycoprotein tenascin‐R (TN‐R) showed an impaired γ‐aminobutyric acid release at perisomatic inhibitory synapses CA1 pyramidal cell layer hippocampus. The present investigated possible ultrastructural correlates abnormal inhibition. Topographic, morphometric, and stereologic methods were applied light...
Significance The development of brain networks is regulated through plasticity and activity-dependent mechanisms that control the continuous formation pruning spine synapses. However, molecular events contribute to these aspects structural remain unclear. By assessing synapse dynamics in rodent hippocampus, we find mediated by a postsynaptic signaling cascade implicating NO, cGMP, vasodilator-stimulated phosphoprotein phosphorylation. Loss this NO mechanism interferes with excitatory...
Mechanisms inducing perforation of the postsynaptic density (PSD) are poorly understood. We show that neural cell adhesion molecule– deficient (NCAM−/−) hippocampal neurons have an abnormally high percentage synapses with perforated PSDs. The PSDs is also increased in wild-type (NCAM+/+) after disruption NCAM/spectrin complex indicating NCAM-assembled spectrin cytoskeleton maintains structural integrity demonstrate PSD perforations contain endocytic zones involved...
Reliable delivery of presynaptic material, including active zone and synaptic vesicle proteins from neuronal somata to terminals, is prerequisite for successful synaptogenesis neurotransmission. However, molecular mechanisms controlling the somatic assembly precursors remain insufficiently understood. We show here that in mutants small GTPase Rab2, both accumulated cell body at trans-Golgi were, consequently, depleted provoking neurotransmission deficits. Ectopic material accumulations...
Synaptic vesicles are organelles that specialize in the storage of a neurotransmitter continuously undergo an exo-endocytotic cycle. During this cycle change their positions within presynaptic terminal and numbers as well spatial arrangement can provide insight into turnover. This article introduces technique based on nearest-neighbor formalism to quantify proximity active zones vesicle clustering different regions terminal. The technique, implemented software package, uses two-dimensional...
Abstract The nucleus of an eukaryotic cell is a membrane‐bound organelle containing major part the cellular genome. Nuclear shape controlled by forces generated in cytoskeleton, nuclear envelope and matrix may change when balance these disturbed. In certain cases, such changes be indicative pathology. feature being commonly addressed both experimental research diagnostics; nevertheless its symmetry‐related aspects receive little attention. This article introduces technique allowing to...
Fine-needle aspiration (FNA) cytology is widely used to examine thyroid lesions. However, its diagnostic accuracy limited by the narrow choice of cytopathologic markers indicative invasive/metastatic powers a tumor. The aim this study was identify features that may serve as such indicators. We have examined FNA smears 50 histologically proven papillary carcinoma (PTC) cases applying computer-assisted morphometry assess patterns formed PTC cells. Cytokeratine (CK) 8 immunocytochemistry verify...
Запропоновано модифікацію експериментальної моделі хвороби Паркінсона, що передбачає стереотаксичну інфузію розчину ротенону за допомогою оригінального пристрою у центральну зону компактної частини чорної субстанції (ЧС) дорослих щурів лінії Вістар.Встановлено, через 10 діб щільність дофамінергічних (ДА) нейронів ділянці інфузії нейротоксину зменшується майже в 6 разів, до 20,2±3,2 нейронів/мм 2 порівняно з відповідним показником неушкодженій контралатеральній ЧС, 119,0±3,3 .Електронна...
Тонкоголкова аспіраційна біопсія (ТАБ) є найбільш часто використовуваним методом діагностики папілярної карциноми (ПКЩ) та інших уражень щитоподібної залози. Однак цитоморфологічні маркери ПКЩ, що застосовуються зараз у діагностичній ТАБ цитології, недостатньо специфічними. Вони спостерігаються як при злоякісних, так і доброякісних ураженнях, експресуються клітинами не кожного підтипу ПКЩ різною часткою популяції клітин пухлини. Мета полягала в дослідженні нових морфологічних маркерів можуть...
Parkinson’s disease (PD) is a multifactorial disorder characterized mainly by the loss of dopaminergic neurons in substantia nigra brain. The pathogenesis spontaneous PD suggested to be multifactorial, an aberrant immune function being one factors influencing PD-associated neurodegeneration. It was found that negrostriatal astrocytes get involved this process. Astrocytes play vital roles brain homeostasis as well participate local innate response triggered variety insults. are not cells, but...