A5051493356- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Immune Cell Function and Interaction
- Adipokines, Inflammation, and Metabolic Diseases
- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Pancreatic function and diabetes
- Lipid metabolism and biosynthesis
- Peroxisome Proliferator-Activated Receptors
- Mitochondrial Function and Pathology
- Muscle metabolism and nutrition
- Lipid metabolism and disorders
- Bone health and osteoporosis research
- Genetics and Physical Performance
- Fibroblast Growth Factor Research
- Sirtuins and Resveratrol in Medicine
- Infant Health and Development
- Occupational Therapy Practice and Research
- Kruppel-like factors research
- Regulation of Appetite and Obesity
- Evaluation and Performance Assessment
- Growth Hormone and Insulin-like Growth Factors
- Bone health and treatments
- Bat Biology and Ecology Studies
- Salivary Gland Tumors Diagnosis and Treatment
Chapman University
2014-2021
University of California, San Francisco
2012-2020
Yale University
2020
Private University of Trujillo
2020
The University of Texas Medical Branch at Galveston
2020
Calgary Laboratory Services
2020
University of Calgary
2020
San Francisco VA Medical Center
2012-2017
University of Southern California
2009-2016
University of California, Berkeley
2010-2016
The role of AMP-activated protein kinase (AMPK) in promoting fatty acid (FA) oxidation various tissues, such as liver and muscle, has been well understood. However, the AMPK lipolysis FA metabolism adipose tissue controversial. To investigate regulation vivo, we generated mice with adipose-tissue-specific knockout both α1 α2 catalytic subunits (AMPK-ASKO mice) by using aP2-Cre adiponectin-Cre. Both models AMPK-ASKO ablation show no changes desnutrin/ATGL levels but have defective...
Multiple signals have been shown to be involved in regulation of fatty acid (FA) and glucose metabolism contracting skeletal muscle. This study aimed determine whether a Ca(2+)-stimulated kinase, CaMKK, is contraction-induced substrate it does so an AMP-activated protein kinase (AMPK)-dependent manner. Rat hindlimbs were perfused at rest (n = 16), with 3 mM caffeine 15), 2 5-aminoimidazole-4-carboxamide 1-beta-d-ribofuranoside (AICAR; n or during moderate-intensity muscle contraction (MC;...
Myokines are specialized cytokines that secreted from skeletal muscle (SKM) in response to metabolic stimuli, such as exercise. Interleukin-15 (IL-15) is a myokine with potential reduce obesity and increase lean mass through induction of processes. It has been previously shown IL-15 acts glucose uptake SKM cells. However, the downstream signals orchestrating link between signaling have not fully explored. Here we employed mouse C2C12 cell line examine targets IL-15-induced alterations...
Abstract Adipose tissue plays an important role in skeletal homeostasis, and there is interest identifying adipokines that influence bone mass. One such adipokine may be apelin, a ligand for the Gi-G protein-coupled receptor APJ, which has been reported to enhance mitogenesis suppress apoptosis MC3T3-E1 cells primary human osteoblasts (OBs). However, it unclear whether apelin physiological regulating homeostasis vivo. In this study, we compared phenotypes of knockout (APKO) wild-type mice...
Molecular mediators of metabolic processes, to increase energy expenditure, have become a focus for therapies obesity. The discovery cytokines secreted from the skeletal muscle (SKM), termed "myokines," has garnered attention due their positive effects on processes. Interleukin-15 (IL-15) is myokine that numerous and linked PPAR family mitochondrial regulators. Here, we aimed determine importance PPARα and/or PPARδ as targets IL-15 signaling. C2C12 SKM cells were differentiated 6 days...
AMP-activated protein kinase (AMPK) is a fuel sensor in skeletal muscle with multiple downstream signaling targets that may be triggered by increases intracellular Ca(2+) concentration ([Ca(2+)]). The purpose of this study was to determine whether [Ca(2+)] induced caffeine act solely via AMPKα(2) and essential increase glucose uptake, fatty acid (FA) FA oxidation contracting muscle. Hindlimbs from wild-type (WT) or dominant-negative (DN) transgene mice were perfused during rest (n = 11),...
Epidemiological studies show that high circulating levels of adiponectin are associated with low bone mineral density. The effect on skeletal homeostasis, osteoblasts in particular, remains controversial. We investigated this issue using mice adipocyte-specific over-expression (AdTg). MicroCT and histomorphometric analysis revealed decreases (15%) fractional volume AdTg at the proximal tibia no changes distal femur. Cortical thickness mid-shafts tibiofibular junction was reduced (3-4%) mice....
AMP-activated protein kinase (AMPK) has been studied extensively and postulated to be a target for the treatment and/or prevention of metabolic disorders such as insulin resistance. Exercise training deemed beneficial obesity Furthermore, exercise is feasible method combat high-fat diet (HFD)-induced alterations in sensitivity. The purpose this study was determine whether AMPK-α2 activity required gain effects with feeding. Wild-type (WT) dominant-negative (DN) male mice were fed standard...
Insulin is important in the regulation of muscle metabolism. However, its role long-chain fatty acid (LCFA) metabolism, independent glucose, not clear. To determine whether insulin regulates LCFA metabolism glucose and if so, via which signaling pathway, L6 myotubes were incubated, presence or absence (100 nM) with either an inhibitor phosphatidylinositol 3-kinase (PI3K) (wortmannin (W), 50 nM), protein kinase B (PKB)/Akt (A, 10 μM), atypical C-ζ (aPKC-ζ) (mP, 100 μM). kinetic parameters...
Myokines are specialized cytokines that secreted from skeletal muscle (SKM) in response to metabolic stimuli, such as exercise. Interleukin‐15 (IL‐15) is a myokine with potential reduce obesity and increase lean mass through induction of processes. It has been previously shown IL‐15 acts glucose uptake SKM cells. However, the downstream signals orchestrating link between signaling have not fully explored. Here we employed mouse C2C12 cell line examine targets IL‐15‐induced alterations...
Owing to its critical role in the regulation of skeletal muscle metabolism, AMP-activated protein kinase (AMPK) remains a central focus research for treatment insulin resistance. The purpose present study was determine AMPKα2 activity glucose uptake and fatty acid (FA) metabolism insulin-resistant muscle. Male C57BL/6 mice were divided into groups fed control diet (CD) or high-fat (60%) (HFD) 6 weeks either wild-type (WT) possessed an dominant negative transgene (DN). After weeks, hindlimbs...
Evidence shows that exercise increases insulin-sensitive glucose uptake and exercise-induced AMP-regulated protein kinase (AMPK) activation is a likely candidate to mediate this metabolic adaptation. The purpose of study was determine whether repeated AMPK can similarly enhance fatty acid (FA) metabolism. L6 myotubes were incubated under the following conditions: plus acute 5-aminoimidazole-4-carboxamide-1- β-d-ribofuranoside (AICAR) treatment (RAA; 1 mmol/L AICAR for 5 h/d days 60 min on...
Abstract The purpose of this study was to determine the minimum amount resistance exercise that would stimulate bone formation yielding an elevation in mineral density (BMD) during growth period female rats. Female rats were randomly divided into: Control (Con, n=8), 3 ladder climb resistance-trained group (3LC, 4 (4LC, 5 (5LC, and 6 (6LC, n=8). All exercised groups conditioned a vertical with weights appended their tail days/wk for total wks. After wks, left tibia BMD (g/cm2) significantly...
It is known that an obesogenic phenotype promotes disease progression as well inflammatory processes. Yoga has come to light a potential alternative “traditional” endurance exercise fight obesity and its associated disorders. been determined yoga interventions have reduced attrition rates compared other modalities of exercise. Importantly, research suggests the ability lower fat mass along‐side reductions in markers. However, molecular mechanism by which acts mediate adiposity not clear...
Obesity is an ever growing epidemic in our society and shows no signs of decelerating prevalence. Lifestyle interventions have proven successful the short-term, however, maintenance has to be a major roadblock. As such, multiple efforts turned molecular regulators metabolism as likely therapies for obesity. The exercise induced myokine, interleukin-15 (IL-15), been established activator mitochondrial activity, through induction expression transcriptional metabolism. One downstream target...
AMPK has been implicated in the regulation of multiple metabolic signaling pathways and deemed an energy sensor during muscle contraction. The purpose this study was to determine if AMPKα2 is required measure alterations activation state molecules caffeine treatment (C) moderate intensity contraction (MIMC). Male C57BL/6 mice were either wild type (WT) or skeletal heart specific dominant negative (DN) AMPKα2. Hindquarters perfused with 550μM palmitate 6mM glucose at rest (R; WT n=5 DN n=6),...
Obesity is a major contributor to the onset and progression of many diseases, such as cardiovascular disease (CVD). Diet exercise are known be successful treatments for obesity ultimately CVD. However, implementation lifestyle interventions difficult maintain. Thus, focus has turned examine role molecular mediators physical activity prevent its related disorders. Following exercise, factors secreted into circulation act regulate various metabolic processes. Interleukin‐IL‐15 (IL‐15) myokine,...