A5049254108- Gold and Silver Nanoparticles Synthesis and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Biosensors and Analytical Detection
- Advanced MEMS and NEMS Technologies
- Advanced biosensing and bioanalysis techniques
- Mechanical and Optical Resonators
- Electronic Packaging and Soldering Technologies
- Acoustic Wave Resonator Technologies
- Protein Interaction Studies and Fluorescence Analysis
- 3D IC and TSV technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanomaterials and Printing Technologies
- Semiconductor materials and devices
- Electrowetting and Microfluidic Technologies
- Microfluidic and Bio-sensing Technologies
- Analytical Chemistry and Sensors
- Microbial Natural Products and Biosynthesis
- Antibiotic Resistance in Bacteria
- Ocean Acidification Effects and Responses
- Advanced Nanomaterials in Catalysis
- Quantum Dots Synthesis And Properties
- Integrated Circuits and Semiconductor Failure Analysis
- Genetics, Bioinformatics, and Biomedical Research
- Nanoplatforms for cancer theranostics
- Nanocluster Synthesis and Applications
Qingdao Institute of Bioenergy and Bioprocess Technology
2020-2025
Chinese Academy of Sciences
2020-2025
Fujian Agriculture and Forestry University
2025
University of Chinese Academy of Sciences
2020-2022
Key Laboratory of Nuclear Radiation and Nuclear Energy Technology
2020
Qingdao Center of Resource Chemistry and New Materials
2020
Qingdao National Laboratory for Marine Science and Technology
2020
Xiamen University
2011-2019
Collaborative Innovation Center of Chemistry for Energy Materials
2014-2019
Leibniz Institute of Photonic Technology
2017-2018
Proteins perform vital functional and structural duties in living systems, the in-depth investigation of protein its native state is one most important challenges postgenomic era. Surface-enhanced Raman spectroscopy (SERS) can provide intrinsic fingerprint information samples with ultrahigh sensitivity but suffers from reproducibility reliability issues. In this paper, we proposed an iodide-modified Ag nanoparticles method (Ag IMNPs) for label-free detection proteins. The silver huge...
Local microenvironment pH sensing is one of the key parameters for understanding many biological processes. As a noninvasive and high sensitive technique, surface-enhanced Raman spectroscopy (SERS) has attracted considerable interest in detection local live cells. We herein develop facile way to prepare Au-(4-MPy)-BSA (AMB) nanosensor. The 4-MPy (4-mercaptopyridine) was used as molecule. modification nanoparticles with BSA not only provides response changes ranging from 4.0 9.0 but also...
Intracellular pH is an important modulator of cell functions, and its subtle change may dramatically affect the cellular activities cause diseases. A reliable imaging intracellular still a great challenge. We imaged during cycle at single living level using newly designed cell-penetrating peptide conjugated nanosensors on home-built in situ microscopic culture platform. The greatly enhanced uptake without sacrificing response. observed gradual alkalization from interphase to prophase rapid...
The increasing application of nanomaterials in biosensor and imaging sets a higher demand on the multifunctionalities for obtaining multiple parameters same system under condition. In this work, multifunctional Au@organosilica nanoparticles with high stability were conveniently synthesized by direct hydrolyzing 3-mercaptopropyltriethoxysilane an aqueous solution presence Au core. Modification core Raman reporters organosilica shell fluorophore before after hydrolysis, respectively, produces...
Raman spectroscopy has received increasing interest in biomedical applications because of its label‐free and vivo detection capabilities. The main challenge for a wider application lies low sensitivity, especially biomolecules. Various strategies have been employed to improve the sensitivity spectroscopy. In this work, we systematically compared two techniques with improved microliter quantities whole human tears: drop‐coating deposition (DCDRS) surface‐enhanced (SERS). High‐quality highly...
A facile procedure was introduced to prepare uniform and highly active substrates assembled with gold nanoparticles for surface-enhanced Raman spectroscopy (SERS). The laser power time dependent SERS study of 4-mercaptopyridine (4-MPy) adsorbed on this substrate investigated. relative intensity the characteristic peak pair (1575 1610 cm–1) found change exposure time. spectral changes may originate from in fraction double-end-bonded single-end-bonded states 4-Mpy nanoparticle-assembled...
Symbiosis with chemoautotrophic bacteria has evolved in many animal lineages inhabiting reducing habitats such as hydrothermal vents, allowing these holobionts to thrive dark biospheres. In certain instances, the symbionts have become intracellular, residing within specialised bacteriocytes. The integration of microbial eukaryotic cells vary across known animals; however, no organelle dedicated chemosynthesis been identified yet. Here, we report a mode symbiosis where sulphur-oxidising...
Antibacterial agents, particularly surfactants, play crucial roles in the personal and home care industries. However, current methods for assessing their efficacy mechanism are commonly time-consuming expensive. Here, we established a ramanome-based approach to investigate antibacterial mechanisms of cationic anionic surfactants with varying alkyl chain lengths against Staphylococcus aureus as model. Our findings further elucidate synergy between acidic pH. Cell membrane integrity was...
We describe a facile and low-cost approach for flexibly integrated surface-enhanced Raman scattering (SERS) substrate in microfluidic chips. Briefly, SERS was fabricated by the electrostatic assembling of gold nanoparticles, shaped into designed patterns subsequent lift-up soft lithography. The micro-pattern could be further within channels conveniently. resulting chip allowed ultrasensitive situ monitoring from transparent glass window. With its advantages simplicity, functionality...
Constructing nanoparticles into well-defined structures at mesoscale and larger to create novel functional materials remains a challenge. Inspired by atomic epitaxial growth, we propose an "epitaxial assembly" method form two-dimensional nanoparticle arrays (2D NAs) directly onto desired materials. As illustration, employ series of surfactant-capped as the "artificial atoms" layered hybrid perovskite (LHP) substrates obtain 2D NAs in large area with few defects. This is universal for...
Abstract Antimicrobial susceptibility tests (ASTs) are pivotal in combating multidrug resistant pathogens, yet they can be time‐consuming, labor‐intensive, and unstable. Using the AST of tigecycline for sepsis as main model, here we establish an automated system Clinical Antimicrobials Susceptibility Test Ramanometry (CAST‐R), based on D 2 O‐probed Raman microspectroscopy. Featuring a liquid robot sample pretreatment machine learning‐based control scheme data acquisition quality control,...
In Raman-activated cell ejection and sequencing (RACE-Seq), success rate sequence coverage have generally been low for shotgun of individual post-RACE cells. Here we quantitatively evaluated the influence lysis condition, nucleic acid amplification parameters Raman measurement on RACE-Seq performance. Variations in laser energy input during signal acquisition, but not duration alkaline lysate lysis, temperature, or under dry aqueous conditions, are vital to multiple displacement (MDA). fact,...
Anodic bonding between glass wafer and Si are widely used for micro-electro-mechanical system (MEMS) device fabrication packaging due to the good quality. The residual stress in interface, which results from different thermal expansion coefficients of two materials, is depended on temperature. In MEMS piezoresistive pressure sensor, anodic bonding, can affect accuracy device, investigated by using finite element method (FEM). sensor performance evaluated effect at temperatures this research....
A surface wetting-driven droplet generation microfluidic chip was developed, and could produce droplets spontaneously once adding a drop of oil an aqueous sample on the without any power source equipment. The is simply composed three drilled holes connected by single microchannel. dropped in middle hole be converged segmented into monodispersed preloading side hole, then flow other through To address high throughput stability practical applications, siphon pump further integrated connecting...
This paper presents the optimized design of membrane dimension and piezoresistors position to obtain a high performance piezoresistive pressure sensor. The designs have been simulated by finite element analysis (FEA). For achieving sensitivity good linearity sensor with measurement span 4 MPa, we find that 25 μm thick diameter 600 for is required. four resistors are aligned along <;110> direction on (001) plane located close rim where induced stress linearly depends nearly maximum membrane.
A hermetic package by silicon-Pyrex7740 anodic bonded structure which has Al electronic contact pads in the bonding area been successfully demonstrated. Silicon wafer was doped with boron at dose about 2×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">20</sup> cm xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> , then were deposited using RF sputtering on silicon and Pyrex7740 respectively. Sufficient mechanical strength test after...
In this paper, a novel decoupled microgyroscopes was designed and simulated. The device named as MS-Gyro is partially structure. trench isolation technology introduced in new design. design consists of drive frame, sense movable electrode, springs connection. electrodes connect with the frame directly are isolated from electrically. ANSYS™ software has been used to simulate two types microgyroscopes. A comparison also made between conventional (CPD) natural frequencies modes designs 8562 Hz...
The experimental characterization for metal-insulator-semiconductor (MIS) capacitor by using the capacitance versus voltage (C-V) measurement is used to investigate characteristics of dielectrics discharging in capacitive micro-actuator. Al/SiNx/n-Si capacitors were prepared measurement. charge relaxation obtained C-V measurements after different electrical stresses MIS capacitors. results show mechanisms electron and hole silicon-rich nitride films. method direct simple be assessing quality...