A5087670253- Mechanical Circulatory Support Devices
- Blood properties and coagulation
- COVID-19 Clinical Research Studies
- Platelet Disorders and Treatments
- Blood Coagulation and Thrombosis Mechanisms
- Dermatological and COVID-19 studies
- Advanced Proteomics Techniques and Applications
- Protease and Inhibitor Mechanisms
- Infectious Aortic and Vascular Conditions
- Infective Endocarditis Diagnosis and Management
- Cardiac Arrest and Resuscitation
- Kawasaki Disease and Coronary Complications
- Retinopathy of Prematurity Studies
- Traumatic Brain Injury and Neurovascular Disturbances
- Clinical Laboratory Practices and Quality Control
- Neonatal and Maternal Infections
- COVID-19 Impact on Reproduction
- Pancreatic function and diabetes
- Advanced Biosensing Techniques and Applications
- Diabetes Management and Research
- Biosensors and Analytical Detection
- Electrochemical sensors and biosensors
- Chronic Kidney Disease and Diabetes
- Dialysis and Renal Disease Management
- Fuel Cells and Related Materials
The University of Melbourne
2021-2024
Murdoch Children's Research Institute
2020-2024
Royal Children's Hospital
2021
To investigate changes in von Willebrand factor (VWF) concentration, function, and multimers during pediatric extracorporeal membrane oxygenation (ECMO) determine whether routine monitoring of VWF ECMO would be useful predicting bleeding.Prospective observational study patients from April 2017 to May 2019.The PICU a large, tertiary referral center.Twenty-five neonates children (< 18 yr) supported by venoarterial ECMO.None.Arterial blood samples were collected within 24 hours pre-ECMO, daily...
Abstract COVID-19 has infected more than 275 million worldwide (at the beginning of 2022). Children appear less susceptible to and present with milder symptoms. Cases children developing clinical features Kawasaki-disease have been described. Here we utilise Mass Spectrometry proteomics determine plasma proteins expressed in healthy pre-pandemic, multisystem inflammatory syndrome (MIS-C) induced ARDS. Pathway analyses were performed affected pathways. 76 are differentially across groups, 85...
OBJECTIVE: To characterize surface-bound proteins and to measure the thickness of fibrin fibers bound extracorporeal membrane oxygenation (ECMO) circuits used in children. DESIGN: Single-center observational prospective study, April November 2021. SETTING: PICU, Royal Children’s Hospital, Melbourne, Australia. PATIENTS: Patients aged less than 18 years on venoarterial ECMO without preexisting disorder. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: were collected from six patients....
Coronavirus disease 2019 (COVID-19) patients have increased thrombosis risk. With increasing age, there is an increase in COVID-19 severity. Additionally, adults with a history of vasculopathy the highest thrombotic risk COVID-19. The mechanisms these clinical differences remain unclear. Human umbilical vein endothelial cells (HUVECs) were infected SARS-CoV-2, influenza A/Singapore/6/86 (H1N1) or mock-infected prior to incubation plasma from healthy children, vasculopathic adults. Fibrin on...
The continuous contact between blood and the foreign surface of extracorporeal membrane oxygenation (ECMO) circuit contributes to hemostatic, inflammatory, other physiological disturbances observed during ECMO. Although previous studies have extensively investigated samples from patients on ECMO, cell adsorption ECMO as an additional factor that could potentially influence clinical outcomes, has largely been overlooked. Here we provide a detailed immunofluorescence (IF) protocol designed...
Purpose of the Study Extracorporeal membrane oxygenation (ECMO) circuit priming procedures are crucial for removing air bubbles and albumin in solution is thought to create a protective layer against blood exposure protein binding.1 However, binding profile ECMO after remains unexplored. This study aims characterize proteins bound priming, corresponding solutions using data-dependent acquisition mass spectrometry (DDA-MS). Main Results Information on information, procedures, collection,...
Abstract Background Type 1 diabetes mellitus (T1DM) is a metabolic disease characterized by dysglycaemia. Cardiovascular (CVD) major complication among T1DM patients and the leading cause of mortality later in life. Methods The study subjects consisted children with poor glycemic control (HbA1c > 7.5%) healthy age gender matched controls. Venous blood samples were collected tested utilizing novel immunoassay panel 96 protein biomarkers. Data analyzed using non‐linear regression analysis...