A5074749862- Bone health and treatments
- Radiopharmaceutical Chemistry and Applications
- HER2/EGFR in Cancer Research
- Estrogen and related hormone effects
- Bone Metabolism and Diseases
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Inflammatory mediators and NSAID effects
- Cancer Treatment and Pharmacology
- Cytokine Signaling Pathways and Interactions
- Microbial metabolism and enzyme function
- Medical Imaging and Pathology Studies
- Prostate Cancer Treatment and Research
- Nuclear Receptors and Signaling
- Circular RNAs in diseases
- Heterotopic Ossification and Related Conditions
- Retinoids in leukemia and cellular processes
- Bone and Dental Protein Studies
- Cancer Immunotherapy and Biomarkers
- Management of metastatic bone disease
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Fibroblast Growth Factor Research
- Axon Guidance and Neuronal Signaling
Inserm
2013-2025
CANTHER - Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers
2025
Centre National de la Recherche Scientifique
2005-2025
Centre Hospitalier Universitaire de Lille
2021-2025
Université de Lille
2011-2025
Université Claude Bernard Lyon 1
2010-2021
Institut de Cancérologie de l'Ouest
2020-2021
Pathophysiology, Diagnosis and Treatment of Bone Diseases
2021
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers
2016-2021
Hôpital Edouard Herriot
2018
Fibrates are widely used hypolipidemic drugs which activate the nuclear peroxisome proliferator-activated receptor (PPAR) alpha and thereby alter transcription of genes controlling lipoprotein metabolism. influence plasma high density its major protein, apolipoprotein (apo) A-I, in an opposite manner man (increase) versus rodents (decrease). In present study we studied molecular mechanisms this species-specific regulation apoA-I expression by fibrates. primary rat human hepatocytes...
Abstract Osteoclasts mediate bone destruction in breast cancer skeletal metastases. Cathepsin K is a proteinase that secreted by osteoclasts and degrades bone. Here, immunohistochemistry revealed cathepsin was expressed not only but also cells metastasize to Following intratibial injection with K–expressing human BT474 cells, tumor-bearing mice treated clinical dosing regimen of inhibitor (CKI; 50 mg/kg, twice daily) had osteolytic lesions were 79% smaller than those the vehicle. The effect...
miRNAs are master regulators of gene expression that play key roles in cancer metastasis. During bone metastasis, metastatic tumor cells must rewire their biology and express genes normally expressed by (a process called osteomimicry), which endow with full competence for outgrowth the marrow. Here, we establish miR-30 family members miR-30a, miR-30b, miR-30c, miR-30d, miR-30e as suppressors breast metastasis regulate multiple pathways, including osteomimicry. Low primary tumors from...
Abstract The nitrogen-containing bisphosphonate zoledronic acid (ZOL), a potent inhibitor of farnesyl pyrophosphate synthase, blocks the mevalonate pathway, leading to intracellular accumulation isopentenyl pyrophosphate/triphosphoric I-adenosin-5′-yl ester 3-(3-methylbut-3-enyl) (IPP/ApppI) metabolites. IPP/ApppI in ZOL-treated cancer cells may be recognized by Vγ9Vδ2 T as tumor phosphoantigens vitro. However, significance these findings vivo remains largely unknown. In this study, we...
Bone metastasis remains a major cause of mortality and morbidity in breast cancer. Therefore, there is an urgent need to better select high-risk patients order adapt patient's treatment prevent bone recurrence. Here, we found that integrin alpha5 (ITGA5) was highly expressed metastases, compared lung, liver, or brain metastases. High ITGA5 expression primary tumors correlated with the presence disseminated tumor cells marrow aspirates from early stage cancer (n = 268; p 0.039). also...
We studied the expression of estrogen-related receptor ERR-1 during mouse embryonic development. mRNA is present in bones formed by both endochondral and intramembranous routes, onset its coincides with bone formation. By RT-PCR experiments, we found that ERR-1, but not related ERR-2, expressed osteoblastic osteosarcoma cell lines as well primary populations derived from normal human bone. gel shift analysis binds a monomer specifically to SFRE sequence (SF-1-responsive-element; TCAAGGTCA)....
The transcription factor TWIST1 induces epithelial-mesenchymal transition and/or escape to the oncogenic-induced failsafe program, facilitating intravasation of breast cancer cells in systemic circulation and their dissemination lungs. Its involvement bone metastasis is unknown. To address this question, human osteotropic MDA-MB-231/B02 were stably transfected with a Tet-inducible vector encoding for TWIST1, whose expression was specifically repressed presence doxycycline (dox)....
Lysyl oxidase (LOX) is a secreted copper-dependent amine whose primary function to drive collagen crosslinking and extracellular matrix stiffness. LOX in colorectal cancer synergizes with hypoxia-inducible factor-1 (HIF-1) promote tumor progression. Here we investigated whether LOX/HIF1 endows cells full competence for aggressive colonization bone. We show that high expression tumors from patients was associated poor clinical outcome, irrespective of HIF-1 In addition, expressed by the bone...
Estrogen receptor-related receptor alpha (ERR alpha) is an orphan nuclear closely related to the estrogen (ER), whose expression covers various stages of embryonic development and persists in certain adult tissues. We show that ERR binds as a homodimer on specific target sequence, SFRE (SF-1 response element), already known respond SF-1. Target sequences are discriminate between SF-1 were identified. have also analyzed transcriptional properties originating from species. All orthologs act...
The orphan nuclear estrogen receptor–related receptor α (ERRα), is expressed by many cell types, but very highly osteoblastic cells in which it transactivates at least one osteoblast-associated gene, osteopontin. To study the putative involvement of ERRα bone, we first assessed its expression rat calvaria (RC) vivo and RC vitro. mRNA protein were all developmental stages from early osteoprogenitors to bone-forming osteoblasts, was most abundant mature cuboidal osteoblasts. assess a...
Bone metastasis is a complication occurring in up to 70% of advanced breast cancer patients. The estrogen receptor-related receptor alpha (ERRα) has been implicated and bone development, prompting us examine whether ERRα may function promoting the osteolytic growth cells bone. In mouse xenograft model metastatic human cancer, overexpression wild-type reduced metastasis, whereas dominant negative mutant promoted metastasis. Osteoclasts were directly affected upregulated osteoclastogenesis...
Adult Ibsp-knockout mice (BSP−/−) display shorter stature, lower bone turnover and higher trabecular mass than wild type, the latter resulting from impaired resorption. Unexpectedly, BSP knockout also affects reproductive behavior, as female do not construct a proper "nest" for their offsprings. Multiple crossing experiments nonetheless indicated that stature weight of BSP−/− mice, since birth throughout life, well femur tibia bones are independent genotype mothers, thus reflect genetic...
Abstract Communication between osteoblasts and osteoclasts plays a key role in bone metabolism. We describe here an unexpected for matrix vesicles (MVs), which bud from bone-forming have well-established initiation of mineralization, osteoclastogenesis. show that the MV cargo miR-125b accumulates matrix, with increased accumulation transgenic (Tg) mice overexpressing osteoblasts. Bone formation Tg are normal, but number bone-resorbing is reduced, leading to higher trabecular mass. targets...
Background information . Sema‐7A is a glycosylphosphatidylinositol‐anchored semaphorin that was first identified in the immune system. It member of large family proteins involved axon guidance signalling. expressed myeloid and lymphoid lineage seems to be cytokine expression chemotaxy through its receptor Plexin C1. However, it can promote outgrowth, acting β1 subunit‐containing integrin receptor. Results conclusions In present study, we have investigated regulation function bone cells....
Abstract Purpose: Nitrogen-containing bisphosphonates (N-BP) such as zoledronate and risedronate exhibit antitumor effects. They block the activity of farnesyl pyrophosphate synthase (FPPS) in mevalonate pathway, leading to intracellular accumulation metabolites (IPP/ApppI), which are recognized tumor phosphoantigens by Vγ9Vδ2 T cells. However, mechanisms responsible for T-cell recognition N-BP–treated tumors producing IPP/ApppI remain unclear. Experimental Design: The effects N-BPs on...