A5101934554- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- Neuroblastoma Research and Treatments
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Bioinformatics and Genomic Networks
- Glioma Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Bone Metabolism and Diseases
- Bone health and osteoporosis research
- Retinoids in leukemia and cellular processes
- RNA Research and Splicing
- RNA regulation and disease
- Kruppel-like factors research
- Spine and Intervertebral Disc Pathology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Bioactive Natural Diterpenoids Research
- Cancer Mechanisms and Therapy
- Circular RNAs in diseases
- Pregnancy-related medical research
- Neurological diseases and metabolism
- CRISPR and Genetic Engineering
- Bone and Joint Diseases
- Mesenchymal stem cell research
Jilin University
2014-2020
Union Hospital
2017-2020
China-Japan Friendship Hospital
2018-2020
Union Hospital
2019-2020
To reveal the potential molecular mechanism of glioblastoma multiforme (GBM) and provide candidate biomarkers for GBM gene therapy. Microarray dataset GSE50161 was obtained from GEO database. The differentially expressed genes (DEGs) were identified between samples control samples, followed by module partition analysis based on WGCNA. Then, pathway functional enrichment analyses DEGs performed. hub further investigated, survival data validation. A total 1913 investigated two groups, 5...
The present study aimed to identify differentially expressed genes (DEGs) regulated by transcription factors (TFs) in glioblastoma, conducting a bioinformatics analysis. results of the may provide potential therapeutic targets that are involved development glioblastoma. GSE4290 raw data set was downloaded from Gene Expression Omnibus database, and consisted 23 non‑tumor samples 77 glioblastoma (grade 4) tumor samples. Robust Multichip Averaging used DEGs between Functional enrichment...
The present study aimed to investigate changes at the transcript level that are associated with spontaneous astrocytoma progression, and further, discover novel targets for glioma diagnosis therapy. GSE4290 microarray data downloaded from Gene Expression Omnibus were used identify differentially expressed genes (DGEs) by significant analysis of (SAM). Short Time Series Miner (STEM) method was then applied class these DEGs based on their degrees differentiation in process tumor progression....
BACKGROUND This study aimed to identify more potential genes and miRNAs associated with the pathogenesis of intracranial aneurysms (IAs). MATERIAL AND METHODS The dataset GSE36791 (accession number) was downloaded from Gene Expression Omnibus database. Differentially expressed (DEGs) were screened for in blood samples patients ruptured IAs controls, followed by functional pathway enrichment analyses. In addition, gene co-expression network constructed significant modules extracted WGCNA R...
Osteosarcoma is one of malignant cancer. Histone phosphorylation common in tumors. We explored the effects p300-CBP-associated factor (PCAF) and H3S28 osteosarcoma cancer cell autophagy.Osteosarcoma lines were collected and/or transfected with full length PCAF or interference miRNAs to mimic silence expression. Immunoprecipitation assay GST pull down was used target targeting H3S28ph. H3-/- SNU-C1 cells H3WT- H3S28F-expressing enhanced green fluorescent protein (EGFP)-tagged LC3 plasmids,...
The present study aimed to elucidate the potential mechanism of cyclin-dependent kinase 2 (CDK2) in neuroblastoma progression and identify candidate genes associated with CDK2 silencing. microarray data GSE16480 were obtained from gene expression omnibus database. This dataset contained 15 samples: Neuroblastoma cell line IMR32 transfected shRNA at 0, 8, 24, 48 72 h (n=3 per group; total=15). Significant clusters differentially expressed (DEGs) identified using fuzzy C‑Means algorithm Mfuzz...
Nuclear apoptosis-inducing factor 1 (NAIF1) acts as an oncogene and involves in tumorigenesis several cancers. However, the expression mechanism of NAIF1 osteosarcoma remains unclear. In this study, we demonstrated downregulation both tissues cell lines. We next explored potential role proliferation migration. The result showed that overexpression evidently suppressed invasion osteosarcoma. Furthermore, investigated mechanisms accounting for dysregulation bioinformatic prediction luciferase...
Circular RNAs (circRNAs) play an important role in the progression of intervertebral disc (IVD) degeneration (IVDD). Using bioinformatics analysis, we have found that expression circRNA hsa_circ_0059955 was significantly downregulated IVDD tissues. However, relevant mechanism remains unclear.CCK-8 and flow cytometry assays were used to evaluate cell proliferation apoptosis. In addition, Western blot assay detect expressions ITCH, p73, CDK2 nucleus pulposus (NP) cells. Moreover, a...
Osteosarcoma (OS) is a cancerous tumor in bone. We aimed to identify the critical genes involved OS progression, and then try elucidate molecular mechanisms of this disease. The microarray data GSE32395 was used for present study. analyzed differentially expressed (DEGs) cells compared with control group by Student’s t-test. significant enriched gene ontology (GO) kyoto encyclopedia genomes (KEGG) pathways were upregulated downregulated genes, respectively. In addition, protein-protein...
The aim of the present study was to identify potential therapeutic target genes and miRNAs for primary myelofibrosis (PMF). dataset GSE53482 downloaded from Gene Expression Omnibus database. differentially expressed (DEGs) (DEMs) peripheral blood (PB) cluster differentiation (CD)34+ cells PMF patients (PB-PMF group) CD34+ healthy individuals (PB-control were analyzed using Linear Models Microarray Data package in R. Kyoto Encyclopedia Genes Genomes used pathway enrichment analysis....
Gene expression data were analysed using bioinformatic tools to demonstrate molecular mechanisms underlying the glioma CpG island methylator phenotype (CIMP). A gene set (accession no. GSE30336) was downloaded from Expression Omnibus, including 36 CIMP+ and 16 CIMP- samples. Differential analysis performed for vs. CIMP‑ samples limma package in R. Functional enrichment subsequently conducted differentially expressed genes (DEGs) Database Annotation, Visualization Integration Discovery....
This study aims to compare the bone mechanical properties of adipose-derived mesenchymal stem cells (ADMSCs) and raloxifene in treating osteoporosis provide related biomechanical basis. The serum animal model was sampled after ADMSCs or intervention measured BALP, TRACP -5b, MMP -9, Cath-K femur taken for compression test, tibia shock vertebrae mineral densitytest, humerus histomorphometric index test. In Group AMSCs, BMD BALP were higher than those MOD, RAL, TRACP-5b, MMP-9 mL-1 lower MOD...
This paper explored the application of RNA in cancer gene therapy. Second, effect recombinant plasmid APE1 siRNA on expression protein was observed. In addition, proliferation cells apoptosis and radiosensitivity human glioma U251 were also discussed. divided into control group, negative group APE1siRNA for culturing. There two holes each independent experiments repeated three times. The detected by Western blot transfection. transfected, while another FAM fluorescent labeled non-sense...