A5087739194- Respiratory viral infections research
- Pneumonia and Respiratory Infections
- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial Infections and Vaccines
- Pediatric health and respiratory diseases
- COVID-19 Clinical Research Studies
- Tracheal and airway disorders
- Bacterial Identification and Susceptibility Testing
- Cancer-related molecular mechanisms research
- Asthma and respiratory diseases
- Kawasaki Disease and Coronary Complications
- Respiratory and Cough-Related Research
- Influenza Virus Research Studies
- Antimicrobial Resistance in Staphylococcus
- Neuroscience of respiration and sleep
- RNA modifications and cancer
- Obstructive Sleep Apnea Research
- Gut microbiota and health
- Fungal Infections and Studies
- Respiratory Support and Mechanisms
- Antifungal resistance and susceptibility
- Genetics and Neurodevelopmental Disorders
- Crystallization and Solubility Studies
Shenzhen Children's Hospital
2012-2025
China Jiliang University
2024
Shantou University Medical College
2022-2023
Shantou University
2022-2023
Cancer Hospital of Shantou University Medical College
2022
Beijing Children’s Hospital
2019
Capital Medical University
2019
Abstract Objectives To investigate the impact of COVID-19 pandemic measures on hospitalizations and alterations persistence epidemiological patterns 12 common respiratory pathogens in children during after cessation “zero-COVID-19” policy southern China. Methods Respiratory specimens were collected from hospitalized with acute infections at Shenzhen Children’s Hospital January 2020 to June 2024. Twelve detected using multiplex PCR. Data demographic characteristics, pathogen detection rates,...
To describe the characteristics of clinical manifestations and epidemiology children with 2019 novel coronavirus (2019-nCoV) infection.
The roles and mechanisms of long noncoding RNAs (lncRNAs) in T helper 2 (Th2) differentiation from allergic asthma are poorly understood. We aimed to explore a novel lncRNA, LincR-protein phosphatase regulatory subunit B' gamma (PPP2R5C), Th2 mouse model asthma.
Acute necrotizing encephalopathy (ANE) secondary to influenza infection is characterized by fulminant neurological deterioration and a high mortality rate. The underlying mechanisms remain unclear, specific treatments are currently lacking. Therefore, understanding the pathogenesis identifying diagnostic therapeutic targets for influenza-induced ANE crucial. Peripheral blood samples were collected from two groups: influenza-infected patients without (mild) with (severe). Differentially...
To study the etiology of recurrent and persistent wheezing in infants toddlers, identify risk factors for infantile asthma. We retrospectively analyzed data 612 children (aged 0-36 months) hospitalized or between 2019.09.01 2022.08.31. comparatively their clinical, laboratory, imaging, lung-examination different groups. Multivariate logistic regression analysis was used to The etiologies significantly differed (P < 0.05). top 3 causes were viral infections (48.4%), asthma (28.0%), protracted...
This study analyzed the epidemiological characteristics and trends of respiratory syncytial virus (RSV) infections among inpatients with acute (ARI) in a children's hospital Shenzhen City from 2020 to 2023. From January December 2023, multiple reverse transcription polymerase chain reaction (RT-PCR) combined capillary electrophoresis fragment analysis technology was used detect nucleic acids 12 pathogens, including RSV, hospitalized children diagnosed ARI. The patients were divided into six...
ABSTRACT Background The COVID‐19 pandemic and associated non‐pharmaceutical interventions significantly altered the epidemiology of respiratory pathogens. This study aimed to evaluate changes in prevalence distribution pathogens among children with acute infections (ARIs) before after relaxation restrictions Shenzhen, China. Methods enrolled hospitalized ARIs Shenzhen Children's Hospital during epidemic those post‐epidemic period. Demographic data patients were retrieved from electronic...
Abstract Background The imbalanced respiratory microbiota observed in pneumonia causes high morbidity and mortality childhood. Respiratory metagenomic analysis demands a comprehensive microbial gene catalogue, which will significantly advance our understanding of host–microorganism interactions. Results We collected 334 samples from 171 healthy children 76 with pneumonia. catalogue we established comprised 2.25 million non-redundant genes, covering 90.52% prevalent genes. major oropharyngeal...
Background PCV13 introduction in China has led to a significant reduction of vaccine serotype Streptococcus pneumoniae . However, non-vaccine serotypes with highly resistance and invasiveness were often reported the post-pneumococcal conjugate era there was regional differences. Methods A total 669 S. strains collected from respiratory tracts hospitalized children at Shenzhen Children’s Hospital 2021 2022. Antimicrobial (AMR) characteristics assessed through antibiotic susceptibility testing...
The current study aims to investigate the etiology spectrum and clinical characteristics of bronchiectasis in Chinese children.The is designed as a multicenter retrospective study. 193 cases were enrolled 13 centers China between 2008 2017. inclusive must meet well HRCT criteria. Only if both two radiologists confirmed diagnosis, case could be enrolled. that not provide imageology data excluded. entered into specialized system then analyzed.One hundred sixty-nine (87%) found have underlying...
Abstract Background Infectious meningitis/encephalitis (IM) is a severe neurological disease that can be caused by bacterial, viral, and fungal pathogens. IM suffers high morbidity, mortality, sequelae in childhood. Metagenomic next-generation sequencing (mNGS) potentially improve outcomes both pathogen host responses increasing the diagnosis accuracy. Methods Here we developed an optimized mNGS pipeline named comprehensive (c-mNGS) to monitor DNA/RNA pathogens simultaneously applied it 142...
Protracted bacterial bronchitis (PBB) is a chronic purulent which could cause recurrent coughing and wheezing in infants. Based on previous reports, main pathogens caused PBB were identified the patients, but their impacts lung microbiota dysbiosis remain unclear.In this study, bronchoalveolar lavage fluid (BALF) was collected from infants tracheomalacia (TM) younger than 3 years old under instruction of Shenzhen Children's Hospital, 12 samples randomly selected for 16S rDNA analysis each...
Streptococcus pyogenes is a bacterial pathogen that causes wide spectrum of clinical diseases exclusively in humans. The distribution emm type, antibiotic resistance and virulence gene expression for S. varies temporally geographically, resulting distinct disease spectra. In this study, we analyzed patterns among isolates from pediatric patients China investigated the relationship between expression, categories. Forty-two representative emm1.0 emm12.0 strains (n = 20 n 22, respectively)...
Abstract Background Respiratory symptoms are the earliest clinical manifestation of Talaromyces marneffei (TM) infection. In this study, we aimed to improve early identification TM infection in human immunodeficiency virus (HIV)-negative children with respiratory as first manifestation, analyze risk factors, and provide evidence for diagnosis treatment. Methods We retrospectively analyzed six cases HIV-negative system presentation. Results All subjects (100%) had cough hepatosplenomegaly,...
MicroRNA (miRNA) mimics or antagomirs hold great promise for asthma treatment compared with glucocorticoids as mainstay therapy asthma. But the role of miRNA in regulating asthmatic inflammation is largely unclear. We previously reported that miR-3162-3p peripheral blood children was obviously upregulated to healthy children. This study aimed elucidate pulmonary normal and mice well preliminarily explore potential antagomir treatment. A noninvasive whole-body plethysmograph measured airway...
Abstract Background The microtubule-dependent molecular motor protein Kinesin Family Member 2A (KIF2A) is down-regulated in asthmatic human airway epithelium. However, little known about the roles of KIF2A as well possible underlying mechanisms asthma. Methods House dust mite (HDM) extract was administered to establish a murine model expression KIF2A, IL-33 and autophagy pathways were detected. plasmid pCMV-KIF2A used overexpress epithelial cells vitro vivo. IL-4, IL-5, other cytokines...
This study aimed to evaluate the molecular epidemiology and antimicrobial resistance of invasive pneumococcal isolates from children in Shenzhen, China, early stage 13-valent conjugated vaccine (PCV-13) era 2018 2020. Invasive strains were isolated hospitalized with diseases (IPDs) January December The serotype identification, multilocus sequence typing (MLST), antibiotic susceptibility tests performed on all culture-confirmed strains. Sixty-four mainly blood (70.3%). Prevalent serotypes 23F...