A5056573003- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- RNA Interference and Gene Delivery
- Plant and animal studies
- Silk-based biomaterials and applications
- Insect Utilization and Effects
- Molecular Biology Techniques and Applications
- Antimicrobial Peptides and Activities
- Neurobiology and Insect Physiology Research
- Bee Products Chemical Analysis
- Advanced biosensing and bioanalysis techniques
Universidade de São Paulo
2019-2025
Universidade de Ribeirão Preto
2019-2020
Abstract Stingless bees are generalist pollinators distributed through the pantropical region. There is growing evidence that their wild populations experiencing substantial decline in response to habitat degradation and pesticides. Policies for conservation of endangered species will benefit from studies focusing on genetic molecular aspects development behavior. The most common method looking at gene expression real-time quantitative polymerase chain reaction preceded by reverse...
Abstract Insect development is primarily controlled by juvenile hormone (JH) and 20-hydroxyecdysone (20E), which regulate gene cascades leading to changes in phenotype, physiology, behavior. Besides these hormones, microRNAs play a crucial role insect regulating expression at the post-transcriptional level. To advance molecular understanding of holometabolous developmental events, we investigate pupal phase honeybee, Apis mellifera . In this study, assessed profiles genes components JH 20E –...
Abstract Background Most of our understanding on the social behavior and genomics bees other insects is centered Western honey bee, Apis mellifera. The genus Apis, however, a highly derived branch comprising less than dozen species, four which genomically characterized. In contrast, for equally eusocial, yet taxonomically biologically more diverse Meliponini, full genome sequence was so far available single Melipona species only. We present here Frieseomelitta varia , stingless bee that has,...
RNA interference (RNAi) is a powerful gene silencing technology, widely used in analyses of reverse genetics, development therapeutic strategies and generation biotechnological products. Here we present free software tool for the rational design RNAi effectors, named siRNA shRNA designer (SSD). SSD incorporates our previously developed Strand Analysis to construct template DNAs amenable large scale production mono-, bi- trivalent multimeric shRNAs, via vitro rolling circle transcription. We...