A5003567780- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Anaerobic Digestion and Biogas Production
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Microbial metabolism and enzyme function
- Nanocomposite Films for Food Packaging
- Genomics and Phylogenetic Studies
- Bioenergy crop production and management
- Enzyme Catalysis and Immobilization
- Listeria monocytogenes in Food Safety
- Genetics, Bioinformatics, and Biomedical Research
- Microbial Community Ecology and Physiology
- Gene expression and cancer classification
- Recycling and Waste Management Techniques
- Enzyme Structure and Function
- Food composition and properties
- Microbial Fuel Cells and Bioremediation
- Genetics, Aging, and Longevity in Model Organisms
- Enzyme Production and Characterization
- Machine Learning in Materials Science
- Microbial Metabolites in Food Biotechnology
- Food Safety and Hygiene
- Biochemical and biochemical processes
- Paleontology and Stratigraphy of Fossils
North Carolina State University
2015-2024
Oak Ridge National Laboratory
2018-2021
Becton Dickinson (United States)
2020
Bioenergy Life Science (United States)
2018
Oak Ridge Associated Universities
2018
Nitrogenase is the only known biological enzyme capable of reducing N2 to bioavailable NH3. Most nitrogenases use Mo as a metallocofactor, while alternative cofactors V and Fe are also viable. Both geological bioinformatic evidence suggest an ancient origin Mo-based nitrogenase in Archean, despite low concentration dissolved Archean oceans. This apparent paradox would be resolvable if mineral-bound were for nitrogen fixation by diazotrophs. In this study, bioavailability Mo, V, was...
Engineering<italic>Pseudomonas putida</italic>for enhanced protein quality control machinery improves its toxicity tolerance.
ABSTRACT Clostridium ljungdahlii is an important synthesis gas-fermenting bacterium used in the biofuels industry, and a preliminary investigation showed that it has some tolerance to oxygen when cultured rich mixotrophic medium. Batch cultures not only continue grow consume H 2 , CO, fructose after 8% O exposure, but fermentation product analysis revealed increase ethanol concentration decreased acetate compared non-oxygen-exposed cultures. In this study, mechanisms for higher production...
Anaerobic fermentation of lignocellulose occurs in both natural and managed environments, is an essential part the carbon cycle as well a promising route to sustainable production fuels chemicals. Lignocellulose solubilization by mixed microbiomes important these contexts.Here, we report development stable switchgrass-fermenting enrichment cultures maintained at various residence times moderately high (55 °C) temperatures. derived from digester inoculum were incubated 55 °C fed...
Clostridium (Ruminiclostridium) thermocellum is a model fermentative anaerobic thermophile being studied and engineered for consolidated bioprocessing of lignocellulosic feedstocks into fuels chemicals. Engineering efforts have resulted in significant improvements ethanol yields titers although further advances are required to make the bacterium industry-ready. For instance, fermentations at lower pH could enable co-culturing with microbes that optima, augment productivity, reduce buffering...
Over the past year, biology educators and staff at U.S. Department of Energy Systems Biology Knowledgebase (KBase) initiated a collaborative effort to develop curriculum for bioinformatics education. KBase is free web-based platform where anyone can conduct sophisticated reproducible bioinformatic analyses via graphical user interface. Here, we demonstrate utility as education, present set modular, adaptable, customizable instructional units teaching concepts in Genomics, Metagenomics,...
Abstract Background Caldicellulosiruptor bescii , a promising biocatalyst being developed for use in consolidated bioprocessing of lignocellulosic materials to ethanol, grows poorly and has reduced conversion at elevated medium osmolarities. Increasing tolerance fermentation osmolarities is desired enable performance necessary (CBP) biocatalyst. Results Two strains C. showing growth phenotypes osmolarity conditions were identified. The first strain, ORCB001, carried deletion the FapR fatty...
Background: Investigators using metagenomic sequencing to study microbiomes often trim and decontaminate reads without knowing their effect on downstream analyses. Objective: This was designed evaluate the impacts JGI trimming decontamination procedures have assembly binning metrics, placement of MAGs into species trees, functional profiles extracted from complex rhizosphere metagenomes, as well how more aggressive these metrics. Methods: Twenty-three Miscanthus x giganteus metagenomes were...
Genes responsible for the anaerobic catabolism of benzoate in thermophilic archaeon Ferroglobus placidus were expressed lignocellulose-degrading bacterium Caldicellulosiruptor bescii, as a first step to engineering this degrade lignin metabolite. The benzoyl-CoA ligase gene was individually, and combination with reductase putative transporter. This effort also assessed heterologous expression from synthetically designed operon whereby each coding sequence proceeded by unique C. bescii...
Lignocellulosics are a major resource for food packaging materials. Given their highly porous and absorptive nature, the uptake retention of bacteria during processing, transportation, storage presents potential risk outbreaks foodborne disease. Thus, greater understanding how proliferate survive on lignocellulosic surfaces is crucial. The aim this work was to compare growth survivability Salmonella Typhimurium Listeria innocua bleached unbleached paper Two different materials were...
Lignocellulosics are a major resource for food packaging materials. Given their highly porous and absorptive nature, the uptake retention of bacteria during processing, transportation, storage presents potential risk outbreaks foodborne disease. Thus, greater understanding how proliferate survive on lignocellulosic surfaces is crucial. The aim this work was to compare growth survivability Salmonella Typhimurium Listeria innocua bleached unbleached paper Two different materials were...
Lignocellulosic materials are widely used for food packaging due to their renewable and biodegradable nature. However, porous absorptive properties can lead the uptake retention of bacteria during processing, transportation, storage, which pose a potential risk outbreaks foodborne disease. Thus, it is great importance understand how proliferate survive on lignocellulosic surfaces. The aim this research was compare growth survivability Salmonella Typhimurium Listeria innocua bleached...