Abstract With the use of dual-color fluorescent cells and a highly sensitive whole-mouse imaging system with both macro-optics micro-optics, we report here development subcellular real-time cancer cell trafficking in live mice. To observe cytoplasmic nuclear dynamics living mouse, tumor were labeled nucleus green protein red cytoplasm. Dual-color injected by vascular route an abdominal skin flap nude The mice imaged Olympus OV100 CCD camera five objective lenses, parcentered parfocal,...

The mechanism of cancer cell deformation and migration in narrow vessels is incompletely understood. In order to visualize the cytoplasmic nuclear dynamics cells migrating capillaries, red fluorescent protein was expressed cytoplasm, green protein, linked histone H2B, nucleus cells. Immediately after were injected heart nude mice, a skin flap on abdomen made. With color CCD camera, we could observe highly elongated nuclei capillaries living mice. velocities measured by capturing images...

Abstract We report here the genetic engineering of dual-color fluorescent cells with one color in nucleus and other cytoplasm that enables real-time nuclear-cytoplasmic dynamics to be visualized living vivo as well vitro. To obtain cells, red protein (RFP) was expressed HT-1080 human fibrosarcoma green (GFP) linked histone H2B nucleus. Nuclear GFP expression enabled visualization nuclear dynamics, whereas simultaneous cytoplasmic RFP ratios cell shape changes. Thus, total cellular can real...

Abstract Cancer metastasis is the life‐threatening aspect of cancer and usually resistant to standard treatment. We report here a targeted therapy strategy for using genetically‐modified strain Salmonella typhimurium . The S. auxotrophic amino acids arginine leucine. These mutations preclude growth in normal tissue but do not reduce bacterial virulence cells. tumor‐targeting , termed A1‐R, expressing green fluorescent protein (GFP), was administered both axillary lymph popliteal node human...

Abstract In the present report, we show real-time imaging of cancer cell trafficking in lymphatic vessels. Cancer cells labeled with both green fluorescent protein (GFP) nucleus and red (RFP) cytoplasm or GFP only RFP were injected into inguinal lymph node nude mice. The trafficked through vessels where they imaged via a skin flap real time at cellular level until entered axillary node. bright fluorescence microscopic capability Olympus OV100 small-animal system enabled lymphatics. Using...

Abstract Although side effects of cancer chemotherapy are well known, “opposite effects” that enhance the malignancy treated not understood. In this report, we describe induction intravascular proliferation, extravasation, and colony formation by cells, critical steps metastasis, pretreatment host mice with commonly used drug cyclophosphamide. contrast, in unpretreated mice, most cells remained quiescent vessels without extravasation. HT1080 human fibrosarcoma labeled nucleus green...

We report here a new targeting strategy for primary bone tumor and lung metastasis with modified auxotrophic strain of Salmonella typhimurium. have previously developed the genetically-modified S. typhimurium, selected therapy in vivo. Normal tissue is cleared these bacteria even immunodeficient athymic mice no apparent side effects. In this study, tumor-targeting termed A1-R, was administered i.v. to nude which metastasis. Primary obtained by orthotopic intratibial injection 5 x 105...

The purpose of this study was to evaluate the quality and quantity augmented bone following alveolar ridge reconstruction with titanium mesh autogenous particulate graft for implant placement in terms preoperative defect.Forty-one patients (50 sites) rehabilitated between September 2000 May 2009 intraoral or iliac cancellous marrow grafts micro-titanium meshes were enrolled. We classified defects by means shape as complex horizontal-vertical (HV), horizontal (H), socket (S) types, evaluated...

We investigated the cell-killing efficacy of UV light on cancer cells expressing GFP in nucleus and RFP cytoplasm (dual-color cells). After exposure to various doses UVA, UVB, or UVC, apoptotic viable were quantitated under fluorescence microscopy using dual-color 143B human osteosarcoma cells, HT-1080 fibrosarcoma Lewis lung carcinoma (LLC), XPA-1 pancreatic vitro. UV-induced cell death was wave-length dose dependent, as well cell-line dependent. UVA exposure, most even when increased up...

Accidental displacement of a dental implant into the maxillary sinus is an infrequent although not uncommon complication encountered in clinical practice, with main cause thought to be inadequate bone height posterior maxilla. We report case migration sinus, and discuss benefits its removal by combination endoscopically assisted repositioning techniques.A 35-year-old Japanese man partially edentulous maxilla underwent placement at private clinic. Three months later, time abutment connection,...

This study aimed to investigate how the chemical elements in relation octacalcium phosphate (OCP) hydrolysis affect osteoblastic differentiation presence of serum fetuin. The adsorption fetuin onto OCP was examined buffers having different degrees supersaturation (DS) with respect and hydroxyapatite (HA) at pH 7.4 37 °C. mesenchymal stem cells (MSCs) evaluated cultures 0 0.8 mg/mL amount adsorbed increased increasing DS buffer. In MSC culture, coexistence 0.2–0.4 close level alkaline...

Treatment of osseous temporomandibular joint ankylosis often requires a surgical procedure. While postoperative rehabilitation is important for prevention relapse, the contributions and period such therapy remain unclear. The present study was performed to evaluate effects on range mouth opening after TMJA surgery. Twelve patients who underwent surgery gap arthroplasty or interpositional were enrolled. course maximal incisal in divided by method, size, rehabilitation, particularly relapse...

Octacalcium phosphate (OCP) has been shown to exhibit an osteogenic property and, therefore, utilized recently as a bone substitute, clinically. However, the stimulatory capacity for induced pluripotent stem (iPS) cells is not known. This study investigated whether OCP enhances osteoblastic differentiation of three-dimensionally cultured spheroids iPS compared hydroxyapatite (HA) and β-tricalcium (β-TCP). Mouse were mixed with smaller (less than 53 μm) or larger (300-500 sizes calcium (CaP)...

To evaluate the roles of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in tumor progression, expression MMP-1, -2 -9 TIMP-1 was analyzed ameloblastomas as well tooth germs.Frozen sections seven germs 22 were immunohistochemically examined using anti-MMP-1, anti-TIMP-1 antibodies.MMP-1, expressed strongly mesenchymal components germs, stromal cells ameloblastomas. Immunoreactivity for MMP-9 significantly stronger than dental follicles papillae. Dental laminae showed...

Abstract We used dual-color in vivo cellular imaging to visualize trafficking, nuclear-cytoplasmic dynamics, and the viability of cancer cells after their injection into portal vein mice. For these studies, we fluorescent that express green protein (GFP) linked histone H2B nucleus retroviral red (RFP) cytoplasm. Human HCT-116-GFP-RFP colon mouse mammary tumor (MMT) were nude The observed intravitally liver at single-cell level using Olympus OV100 whole-mouse system. Most remained sinusoids...

Abstract We have developed a new in vivo mouse model to image single cancer‐cell dynamics of metastasis the lung real‐time. Regulating airflow volume with novel endotracheal intubation method enabled controlling expansion adequate for imaging exposed surface. Cancer cells expressing green fluorescent protein (GFP) nucleus and red (RFP) cytoplasm were injected tail vein mouse. The right chest wall was then opened order metastases on surface directly. After each observation, sutured air...

It has been suggested that resonance frequency analysis (RFA) can measure changes in the stability of dental implants during osseointegration. This retrospective study aimed to evaluate implant at time surgery (primary stability; PS) and secondary (SS) after ossseointegration using RFA, investigate relationship between cortical bone thickness.In total, 113 patients who attended Tohoku University Hospital Dental Implant Center were included this study. A total 229 placed either mandibular...