A5103060981- Semiconductor materials and devices
- Advancements in Semiconductor Devices and Circuit Design
- Copper Interconnects and Reliability
- Retinoids in leukemia and cellular processes
- Acute Myeloid Leukemia Research
- Bone fractures and treatments
- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Cancer Cells and Metastasis
- Nanowire Synthesis and Applications
- Acute Lymphoblastic Leukemia research
- Electronic Packaging and Soldering Technologies
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
- Semiconductor materials and interfaces
- Peroxisome Proliferator-Activated Receptors
- interferon and immune responses
- DNA Repair Mechanisms
- Regulation of Appetite and Obesity
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Integrated Circuits and Semiconductor Failure Analysis
- Immune Cell Function and Interaction
Kanazawa University
1989-2024
Tottori University
2007-2023
Kanazawa University Hospital
2015-2023
Shinshu University Hospital
2012-2022
Gunma Children's Medical Center
2022
Renesas Electronics (Japan)
2011-2020
Hitachi (Japan)
1994-2020
Kyoto Pharmaceutical University
2011-2019
Children's Medical Center
2016
Osaka University
2016
Abstract Bile acid (BA) metabolism is tightly controlled by nuclear receptor signaling to coordinate regulation of BA synthetic enzymes and transporters. Here we reveal a molecular cascade consisting the antiapoptotic protein BCL2, Shp long non-coding RNA (lncRNA) H19 maintain homeostasis. Bcl2 was overexpressed in liver C57BL/6J mice using adenovirus mediated gene delivery for two weeks. Hepatic overexpression caused drastic accumulation serum bilirubin levels dysregulated reactivation...
The mechanism of cancer cell deformation and migration in narrow vessels is incompletely understood. In order to visualize the cytoplasmic nuclear dynamics cells migrating capillaries, red fluorescent protein was expressed cytoplasm, green protein, linked histone H2B, nucleus cells. Immediately after were injected heart nude mice, a skin flap on abdomen made. With color CCD camera, we could observe highly elongated nuclei capillaries living mice. velocities measured by capturing images...
Recently, the demands to achieve both high-speed and high-quality imaging including high A/D resolution have increased. The new target specification is 60fps Ultra-High-Definition with 12b resolution. This requires 24Gb/s, while reported CMOS image sensors reached up 6.5Gb/s. paper presents a 34.8Gb/s sensor quality, which realizes 17.7M pixels at 120fps dynamic range of over 75dB (one highest qualities, compared data from). Generally there exists trade-off between quality. because brings...
We show for the first time that potent microRNA-433 (miR-433) inhibition of expression cAMP response element-binding protein CREB1 represses hepatocellular carcinoma (HCC) cell migration. identified a miR-433 seed match region in human and mouse 3'-UTRs. Overexpression markedly decreased 3'-UTR reporter activity, inhibitory effect was alleviated upon mutation its binding site. Ectopic reduced levels variety cancer cells, including HeLa, Hepa1, Huh7, HepG2. Human highly invasive MHCC97H cells...
Abstract Hepatocellular carcinoma (HCC) is one of the leading causes cancer death worldwide. Cancer stem cells (CSCs) have attracted attention as a novel therapeutic target for because they play important roles in development and aggravation cancer. CD44 expressed standard isoform (CD44s) several variant isoforms. CD44v major on CSCs variety tumors has been extensively studied. However, HCC tissues dominantly express CD44s, whose function remains unclear. In present study, we investigated...
A long noncoding RNA (lncRNA), nuclear enriched abundant transcript 1 (NEAT1) variant (NEAT1v1), is involved in the maintenance of cancer stem cells (CSCs) hepatocellular carcinoma (HCC). CSCs are suggested to play important roles therapeutic resistance. Therefore, we investigated whether NEAT1v1 sensitivity radiation therapy HCC. Gene knockdown was performed using short hairpin RNAs, and NEAT1v1-overexpressing HCC cell lines were generated by stable transfection with a NEAT1v1-expressing...
A human disorder caused by mutation in nonmuscle actin has not been reported. We report here a variant of female patient with recurrent infections, photosensitivity, and mental retardation. She also had abnormalities neutrophil chemotaxis, superoxide production, membrane potential response. Two-dimensional PAGE analysis proteins from neutrophils other cell types this demonstrated unique protein spot migrating at 42 kDa pI shifted slightly to neutral relative normal β- γ-actin. Digestion...
Human bone marrow-derived mesenchymal stem cells (BM-MSCs) are expected to be a potential source of for transplantation. Although recent reports have shown that isolated MSCs can differentiate into hepatocytes, the efficiency differentiation is insufficient therapeutic application. To circumvent this problem, it necessary understand mechanisms hepatic human BM-MSCs. Hepatocyte nuclear factor 3beta (HNF3beta), forkhead/winged helix transcription factor, essential liver development. In present...
Astaxanthin (Asx) is known to be a potent quencher of singlet oxygen and an efficient scavenger superoxide anion. Therefore, Asx would expected useful antioxidant for the prevention oxidative stress, causative factor in severe diseases such as ischemic reperfusion injury. However, it still unclear whether has scavenging capability against most reactive species (ROS), hydroxyl radical, because hydrophobicity prevents analysis radical ability aqueous solution. In this study, solve problem,...
The patients with non-alcoholic fatty liver disease (NAFLD) have been reported to be at greater risk for progression chronic including cirrhosis (LC). To examine the mechanisms of NAFLD, a genetic analysis hepatic expression profile in retinoid metabolism NAFLD was performed since loss signaling is associated via reactive oxygen species (ROS) generation.Fifty-one genes, which are and action, were examined thirty six subjects 17 simple steatosis, 11 steatohepatitis (NASH) eight controls by...
In mammals, circadian rhythms are essential for coordinating the timing of various metabolic processes. The Clock gene regulates diurnal plasma triglyceride fluctuation through nuclear receptor small heterodimer partner ( Shp ; Nr0b2). Given that SHP is a critical regulator metabolism in liver, it unknown whether necessary to coordinate and rhythms. S hp +/ + −/− mice on C57BL/6 background (n = 3‐5/group) were fed standard chow diet water ad libitum . Serum livers collected at zeitgeber time...
Transgenic mice expressing dominant-negative retinoic acid receptor (RAR) α specifically in the liver exhibit steatohepatitis, which leads to development of tumors. Although cause steatohepatitis these is unknown, diminished hepatic expression insulin-like growth factor-1 suggests that insulin resistance may be involved. In present study, we examined effects retinoids on gain further insight into mechanisms responsible for this condition. Dietary administration all - trans -retinoic (ATRA)...
Resistin, an adipose-tissue-specific secretory factor, aggravates metabolic syndrome through impairment of glucose metabolism. Previously, we demonstrated that resistin expression was induced in both 3T3-L1 cells and primary pre-adipocytes derived from Zucker obese rats during the process differentiation maturation (Ikeda Y, Hama S, Kajimoto K, Okuno T, Tsuchiya H & Kogure K (2011) Biol Pharm Bull 34, 865-870). However, biological function adipocytes is poorly understood. In present study,...
Background/Aim: The alkylating agent trabectedin, which binds the minor groove of DNA, is second-line therapy for soft-tissue sarcoma but has only moderate efficacy. aim present study was to determine synergistic efficacy recombinant methioninase (rMETase) and trabectedin on fibrosarcoma cells in vitro, compared with normal fibroblasts. Materials Methods: HT1080 human expressing green fluorescent protein (GFP) nucleus red (RFP) cytoplasm Hs27 fibroblasts, were used. Each cell line cultured...
Background & AimsWe previously reported that impaired retinoid signaling in the liver causes steatohepatitis and hepatocellular carcinoma. Recently, oxidative stress induced by hepatic iron overload has emerged as an important factor for progression of disease patients with chronic hepatitis C, alcoholic disease, nonalcoholic steatohepatitis. In this study, relationship between metabolism was investigated.MethodsThe effect retinoids on examined HuH7 cells treated all-trans retinoic acid...
CD44, a cancer stem cell (CSC) marker, is required for maintaining CSC properties in hepatocellular carcinoma (HCC). Nuclear enriched abundant transcript 1 (NEAT1), long noncoding RNA (lncRNA), an oncogenic driver HCC. In the present study, we investigated significance of NEAT1 gene association with CD44 expression liver CSCs human HCC lines. The were evaluated by spheroid culture, marker expression, and sensitivity to anti-cancer drugs. both variant (NEAT1v1) 2 (NEAT1v2) as well was...
A long noncoding RNA, nuclear paraspeckle assembly transcript 1 (NEAT1) variant (NEAT1v1), confers radioresistance to hepatocellular carcinoma (HCC) cells by inducing autophagy via γ-aminobutyric acid receptor-associated protein (GABARAP). Radiation induces oxidative stress damage cellular components and organelles, but it remains unclear how NEAT1v1 protects HCC from radiation-induced autophagy. To address this, we precisely investigated NEAT1v1-induced in irradiated cell lines. X-ray...