A5039361933- Protease and Inhibitor Mechanisms
- Bone and Dental Protein Studies
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Wnt/β-catenin signaling in development and cancer
- Liver physiology and pathology
- Lymphatic System and Diseases
- Retinoids in leukemia and cellular processes
- Lung Cancer Diagnosis and Treatment
- Galectins and Cancer Biology
- Lung Cancer Research Studies
- Cancer, Hypoxia, and Metabolism
- Breast Lesions and Carcinomas
- Pancreatic function and diabetes
- Mesenchymal stem cell research
- interferon and immune responses
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- Cytokine Signaling Pathways and Interactions
- Cancer-related molecular mechanisms research
- Trace Elements in Health
- Iron Metabolism and Disorders
Tottori University
2015-2024
Tottori University Hospital
2010
Abstract Accumulating evidence has indicated that immune regulatory cells are involved in the establishment of tumoral evasion. However, role B (Bregs) this remains unclear. Here, we identified a for Bregs evasion gastric cancer (GC) patients. The frequency peripheral was significantly higher GC patients than healthy controls ( P = 0.0023). Moreover, CD19 + CD24 hi CD27 tissue blood and tissue. Carboxyfluorescein succinimidyl ester labeling revealed could suppress proliferation autologous...
Sorafenib, a multi‐kinase inhibitor, is the only standard clinical drug for patients with advanced hepatocellular carcinoma ( HCC ); however, development of sorafenib resistance in often prevents its long‐term efficacy. Therefore, novel targets and strategies are urgently needed to improve antitumor effect sorafenib. In present study, we examined mechanisms cells by investigating difference sensitivity between two cell lines. Sorafenib induced more apoptosis HepG2 compared Hep3B cells....
Abstract Hepatocellular carcinoma (HCC) is one of the leading causes cancer death worldwide. Cancer stem cells (CSCs) have attracted attention as a novel therapeutic target for because they play important roles in development and aggravation cancer. CD44 expressed standard isoform (CD44s) several variant isoforms. CD44v major on CSCs variety tumors has been extensively studied. However, HCC tissues dominantly express CD44s, whose function remains unclear. In present study, we investigated...
Human bone marrow-derived mesenchymal stem cells (BM-MSCs) are expected to be a potential source of for transplantation. Although recent reports have shown that isolated MSCs can differentiate into hepatocytes, the efficiency differentiation is insufficient therapeutic application. To circumvent this problem, it necessary understand mechanisms hepatic human BM-MSCs. Hepatocyte nuclear factor 3beta (HNF3beta), forkhead/winged helix transcription factor, essential liver development. In present...
Transgenic mice expressing dominant-negative retinoic acid receptor (RAR) α specifically in the liver exhibit steatohepatitis, which leads to development of tumors. Although cause steatohepatitis these is unknown, diminished hepatic expression insulin-like growth factor-1 suggests that insulin resistance may be involved. In present study, we examined effects retinoids on gain further insight into mechanisms responsible for this condition. Dietary administration all - trans -retinoic (ATRA)...
Background & AimsWe previously reported that impaired retinoid signaling in the liver causes steatohepatitis and hepatocellular carcinoma. Recently, oxidative stress induced by hepatic iron overload has emerged as an important factor for progression of disease patients with chronic hepatitis C, alcoholic disease, nonalcoholic steatohepatitis. In this study, relationship between metabolism was investigated.MethodsThe effect retinoids on examined HuH7 cells treated all-trans retinoic acid...
Human umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) are expected to be an excellent source of for transplantation. In addition, the cell plasticity human UCBMSCs, which can transdifferentiate into hepatocytes, has been reported. However, mechanisms involved remain clarified. To identify genes and/or signals that important in specifying hepatic fate we analyzed gene expression profiles during differentiation UCBMSCs with telomerase reverse transcriptase, immortalized by...
Background/Aim: Podoplanin is a candidate cancer stem cell marker in squamous carcinoma (SCC). Several studies have reported the prognostic value of podoplanin expression tumor cells lung SCC but few focused on its cancer-associated fibroblasts (CAFs). The aim this study was to analyze significance expression, with special reference pattern both and CAFs. Patients Methods: Immunohistochemical analyses using anti-podoplanin antibody were performed 126 resected specimens SCC. When more than...
Bone marrow-derived mesenchymal stem cells (MSC) are expected to be an excellent source of for transplantation. We aimed study the culture conditions and involved genes differentiate MSC into hepatocytes.The induce efficient differentiation human bone UE7T-13 were examined using cytokines, hormones, 5-azacytidine type IV collagen.We found that combination acidic fibroblast growth factor (aFGF), basic (bFGF) hepatocyte (HGF) with collagen coating induced hepatic at over 30% frequency, where...
Abstract Background & Aims Cancer stem cells ( CSC s) play a pivotal role in progression, metastasis and recurrence of cancer. Therefore, it is clinically useful to identify the relevant marker that associated with prognosis hepatocellular carcinoma HCC ) clarify its genetic biological characteristics. Methods Expression four markers, CD 13, Ep CAM , 44 44v9, was examined 99 patients. Biological cDNA /mi RNA microarray data were compared among 44‐positive/‐negative normal hepatic cells....
Background/Aim: The presence of cancer stem cells (CSCs) contributes to metastasis, recurrence, and resistance chemo/radiotherapy in hepatocellular carcinoma (HCC). WNT signaling pathway is reportedly linked the maintenance stemness CSCs. In present study, order eliminate liver CSCs improve prognosis patients with HCC, we explored whether small-molecule compounds targeting suppress Materials Methods: screening was performed using cell proliferation assay reporter assay. We next investigated...
Non-small cell lung cancer (NSCLC) accounts for >85% of incidences cancer, which the predicted 5‑year survival rates are low and recurrence remain high. Although it has been reported that patients with SCLC cells possess cluster differentiation (CD) 117 marker exhibited poor prognosis response to chemotherapy, no studies concerning association CD117 expression NSCLC have reported. An in vitro study reportedly revealed CD117‑positive populations lines stem (CSC) phenotypes including...
Podoplanin expression in cancer-associated fibroblasts (CAFs) has been proposed as an unfavourable indicator squamous cell carcinoma of the lung, but little is known about its clinical significance early-stage lung adenocarcinoma. We evaluated prognostic impact podoplanin patients with pathological stage (p-stage) IA adenocarcinoma categorised by 8th edition tumour-node-metastasis classification for cancer.Immunohistochemical analyses using anti-podoplanin antibody were performed on resected...
We aimed to analyze the clinical impact of solid and micropapillary components in a series Japanese patients resected for ≤3 cm lung adenocarcinoma.A total 115 with adenocarcinomas were reviewed classified according American Thoracic Society European Respiratory classification. The presence (S+) or component (MP+) was defined when constituted ≥1% entire tumor. these on disease-free (DFS) disease-specific (DSS) survival analyzed.Thirty (26.1%) cases S+ 27 (23.5%) MP+ identified, multivariate...
Abstract Mammary serine protease inhibitor (maspin) is a tumor suppressor gene that downregulated during carcinogenesis and breast cancer progression. While the nuclear localization of maspin essential for suppression, we previously reported cytoplasmic was significantly correlated with poor prognosis in patients. To understand mechanisms underlie oncogenic role maspin, studied its biological function cell lines. Subcellular MDA-MB-231 cells mainly detected cytoplasm, whereas MCF10A mammary...
Telomerase reverse transcriptase ( TERT ) promotes immortalization by protecting telomeres in cancer cells. Mutation of the promoter is one most common genetic alterations hepatocellular carcinoma HCC ), indicating that upregulation a critical event hepatocarcinogenesis. Regulators transcription are, therefore, predicted to be plausible targets for treatment. We undertook genome‐wide sh RNA library screen and identified C15orf55 C7orf43 as regulators expression HepG2 Promoter assays showed...
Maspin is known to be a tumour suppressor protein, and its prognostic significance in patients with several types of cancer, including lung squamous cell carcinoma (SCC), has been reported. However, impact on SCC controversial. We explored the value maspin expression particular reference subcellular localization SCC.Paraffin-embedded tissue samples from 101 curatively resected were analysed immunohistochemically using an antibody for maspin. positivity was defined as strong only cytoplasm...
Abstract Background Small cell lung cancer (SCLC) is a progressive disease with poor prognosis. Recently, method to classify SCLC by the expression status of four transcription factors, ASCL1, NEUROD1, POU2F3, and YAP1, was proposed. Here, we investigated potential relationships between these factors effect lurbinectedin. Methods mRNA protein YAP1 were quantified in eight lines analyzed for correlations drug sensitivity. In addition, evaluated 105 resected cases high‐grade neuroendocrine...
Background/Aim: Cancer stem cells (CSCs) are associated with prognosis of hepatocellular carcinoma (HCC). In our previous study, we created cDNA microarray databases on the CSC population human HuH7 cells. present identified genes that might serve as prognostic markers HCC by employing existing databases. Materials and Methods: Expressions glutathione S-transferase pi 1 (GSTP1), lysozyme (LYZ), C-X-C motif chemokine ligand 5 (CXCL5), interleukin-8 (IL8) dickkopf WNT signaling pathway...
Micropapillary carcinoma (MPC), a relatively rare histologic observed in various organs, is associated with vascular invasion, nodal metastasis, and poor prognosis. MPC different from papillary as it has no fibrovascular core thus considered essentially hypovascular. MPCs are known to upregulate glucose transporter 1 (GLUT1) via the activation of transcription factor, hypoxia-inducible factor (HIF)-1. Here we evaluated expression nutrient transporters gain better understanding system used by...
The present authors previously reported that a synthetic retinoid, CD437, induces endoplasmic reticulum stress‐mediated apoptosis in ovarian adenocarcinoma cells spite of no response to natural retinoids. However, the precise mechanism its proapoptotic action has not been fully determined. study herein demonstrates induction SKOV3 by CD437 involves upregulation thioredoxin‐binding protein 2 (TBP2) is dependent on intracellular calcium concentration. TBP2 known bind and suppress thioredoxin...