A5081516321- Hair Growth and Disorders
- Nerve injury and regeneration
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Vascular Tumors and Angiosarcomas
- Cancer Research and Treatments
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Nanoplatforms for cancer theranostics
- Cutaneous lymphoproliferative disorders research
- Cardiac tumors and thrombi
- Skin and Cellular Biology Research
- Cancer and Skin Lesions
- Virus-based gene therapy research
- Wnt/β-catenin signaling in development and cancer
- Mesenchymal stem cell research
- Cancer Cells and Metastasis
- Sarcoma Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- Dermatology and Skin Diseases
- Psoriasis: Treatment and Pathogenesis
- Lymphoma Diagnosis and Treatment
- Contact Dermatitis and Allergies
- Wound Healing and Treatments
- Drug-Induced Adverse Reactions
Kitasato University
2015-2024
Kitasato University Hospital
2017
AntiCancer (United States)
2004-2016
National Sagamihara Hospital
2012
University of California, San Diego
2004-2011
Massachusetts Institute of Technology
2004-2005
We have recently shown that the expression of nestin, neural stem cell marker protein, is expressed in bulge-area cells hair follicle. used transgenic mice with GFP driven by nestin regulatory element [nestin-driven (ND-GFP)]. The ND-GFP give rise to outer-root sheath follicle as well an interfollicular vascular network. In this study, we demonstrate isolated from hair-follicle bulge area are negative for keratinocyte keratin 15 can differentiate into neurons, glia, keratinocytes, smooth...
The hair follicle bulge area is an abundant, easily accessible source of actively growing, pluripotent adult stem cells. Nestin, a protein marker for neural cells, also expressed in cells and their immediate, differentiated progeny. fluorescent GFP, whose expression driven by the nestin regulatory element transgenic mice, served to mark cell fate. nestin-driven GFP are positive CD34 but negative keratinocyte keratin 15, suggesting relatively undifferentiated state. These can differentiate...
Besides forming hair shafts, the highly organized, metabolically vigorous follicle plays several crucial roles in skin architecture. The contains a distinct population of presumptive follicular stem cells that express nestin, also marker for neural cells. These nestin-expressing are located principally bulge region. Nestin-driven GFP (ND-GFP), transfected into mice, labels region, which is consistent with cells' being follicle. We report here ND-GFP developing blood vessels appear to...
AbstractThe mouse hair follicle is an easily accessible source of actively growing, pluripotent adult stem cells. C57BL transgenic mice, labeled with the fluorescent protein GFP, afforded cells whose fate could be followed when transferred to recipient animals. These appear relatively undifferentiated since they are positive for cell markers nestin and CD34 but negative keratinocyte marker keratin 15. can differentiate into neurons, glia, keratinocytes, smooth muscle cells, melanocytes in...
The mechanism of cancer cell deformation and migration in narrow vessels is incompletely understood. In order to visualize the cytoplasmic nuclear dynamics cells migrating capillaries, red fluorescent protein was expressed cytoplasm, green protein, linked histone H2B, nucleus cells. Immediately after were injected heart nude mice, a skin flap on abdomen made. With color CCD camera, we could observe highly elongated nuclei capillaries living mice. velocities measured by capturing images...
Abstract We report here a novel transgenic nude mouse for the visualization of human tumor angiogenesis. have recently shown that neural stem cell marker nestin is expressed in hair follicle cells and blood vessel networks skin C57/B6 mice with regulatory element–driven green fluorescent protein (ND-GFP). Others ND-GFP brain, pancreas, testes these mice. In present study, gene was crossed into on background to obtain spinal cord, stomach, esophagus, heart, lung, vessels glomeruli, skeletal...
Abstract The optimal source of stem cells for regenerative medicine is a major question. Embryonic (ES) have shown promise pluripotency but ethical issues and potential to form teratomas. Pluripotent been produced from skin by either viral‐, plasmid‐ or transposon‐mediated gene transfer. These termed induced pluripotent iPS cells. may also malignant are inefficiently produced. not be suited individualized therapy, since they can undergo immunologic rejection. To address these fundamental...
Nestin has been shown to be expressed in the hair follicle, both bulge area (BA) as well dermal papilla (DP). Nestin-expressing stem cells of BA and DP have previously pluripotent able form neurons other non-follicle cell types. The nestin-expressing from termed skin precursor or SKP cells. objective present study was determine major source follicle compare ability repair spinal cord injury. Transgenic mice which nestin promoter drives GFP (ND-GFP) were used order observe expression DP....
Abstract Male‐pattern hair loss ( MPHL , androgenetic alopecia) is a slowly progressive form of alopecia which begins after puberty. In 2010, we published the first Japanese edition guidelines for diagnosis and treatment . It achieved original goal providing physicians patients in Japan with evidence‐based information choosing efficacious safe therapy Subsequently, new therapeutic drugs methods have been developed, women's perception has undergone change term “female‐pattern FPHL )” becoming...
We have previously demonstrated that the neural stem-cell marker nestin is expressed in hair follicle stem cells located bulge area which are termed hair-follicle-associated pluripotent (HAP) cells. HAP from mouse and human could form spheres culture, spheres, keratin 15-negative CD34-positive differentiate to neurons, glia, keratinocytes, smooth muscle cells, melanocytes vitro. Subsequently, we nestin-expressing effect nerve spinal cord regeneration models. In present study, differentiated...
Cutaneous angiosarcoma (CAS) is a malignant sarcoma with poor prognosis. Programmed cell death-1 (PD-1)/programmed ligand-1 (PD-L1) expression reflects antitumor immunity, and associated patient prognosis in various cancers. The purpose of this study to investigate the relationship between PD-1/PD-L1 CAS cases (n = 106) were immunohistochemically studied for PD-L1 PD-1 expression, correlation was analyzed. assessed by flow cytometry on three lines or without IFNγ stimulation. A total 30.2%...
Abstract We have recently shown that the neural-stem cell marker nestin is expressed in hair follicle stem cells and blood vessel network interconnecting follicles skin of transgenic mice with regulatory element–driven green fluorescent protein (ND-GFP). The were to give rise nestin-expressing vessels skin. In present study, we visualized tumor angiogenesis by dual-color fluorescence imaging ND-GFP after transplantation murine melanoma line B16F10 expressing red protein. was highly...
Abstract Nestin‐positive, keratin 15 (K15)‐negative multipotent hair follicle stem cells are located above the bulge. We have termed this location pluripotent cell area. previously shown that transplantation of nestin‐expressing can regenerate peripheral nerve and spinal cord injuries. In present study, we regenerated impinged sciatic by transplanting cells. Human were transplanted around ICR nude (nu/nu) mice. The between fragments mouse where they differentiated into glial fibrillary...
We have previously reported that nestin-expressing hair follicle stem cells can differentiate into neurons, Schwann cells, and other cell types. In the present study, vibrissa follicles, including their sensory nerve stump, were excised from transgenic mice in which nestin promoter drives green fluorescent protein (ND-GFP mice), placed 3D histoculture supported by Gelfoam®. β-III tubulin-positive fibers, consisting of ND-GFP-expressing extended up to 500 µm whisker stump histoculture. The...
We have previously demonstrated that the neural stem-cell marker nestin is expressed in hair follicle stem cells. Nestin-expressing cells were initially identified bulge area (BA) using a transgenic mouse model which promoter drives green fluorescent protein (ND-GFP). The hair-follicle ND-GFP-expressing are keratin 15-negative and CD34-positive could differentiate to neurons, glia, keratinocytes, smooth muscle melanocytes vitro. Subsequently, we showed nestin-expressing affect nerve spinal...
We have previously reported that hair follicles contain multipotent stem cells which express nestin. The nestin-expressing form the follicle sensory nerve. In vitro, can differentiate into neurons, Schwann cells, and other cell types. present study, sciatic nerve was excised from transgenic mice in nestin promoter drives green fluorescent protein (ND-GFP mice). ND-GFP of were also found to be as follicle. When mouse cultured on Gelfoam® imaged by confocal microscopy, they observed forming...
Nestin-expressing hair-follicle-associated pluripotent (HAP) stem cells are located in the bulge area of follicle. Previous studies have shown that HAP can differentiate to neurons, glia, keratinocytes, smooth muscle cells, and melanocytes vitro. effected nerve spinal cord regeneration mouse models. Recently, we demonstrated differentiated beating cardiac cells. The differentiation potential was greatest upper part beat rate stimulated by isoproterenol. In present study, observed...
Abstract We previously showed that the stem cell marker nestin is expressed in hair follicle cells which suggested their pluripotency. subsequently nestin‐expressing hair‐follicle pluripotent (hfPS) can differentiate culture to neurons, glial cells, keratinocytes, and other types promote regeneration of peripheral nerve spinal cord injuries upon injection injured or cord. The location hfPS has been termed area (hfPSCA). Previously, were cultured for 1–2 months before transplantation would...
Nestin, a marker of neural stem cells, is expressed in the cells mouse hair follicle. The nestin-expressing follicle give rise to outer-root sheath. Nestin-expressing that are negative for keratinocyte keratin 15 (K15) can differentiate into neurons, glia, keratinocytes, smooth muscle and melanocytes vitro. Recent studies suggest epithelial important tumorigenesis. In this study, we immunohistochemically examined expression three cell progenitor markers, nestin, K15, CD34, normal human...