A5053670315- Fungal and yeast genetics research
- Fungal Biology and Applications
- Seismic Waves and Analysis
- Earthquake Detection and Analysis
- Mycorrhizal Fungi and Plant Interactions
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Methane Hydrates and Related Phenomena
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Microbial Natural Products and Biosynthesis
- CRISPR and Genetic Engineering
- Entomopathogenic Microorganisms in Pest Control
- RNA and protein synthesis mechanisms
- Gene Regulatory Network Analysis
- Protist diversity and phylogeny
- Insect symbiosis and bacterial influences
- Yeasts and Rust Fungi Studies
- Cellular transport and secretion
- Fermentation and Sensory Analysis
- Plant Pathogens and Fungal Diseases
- Polysaccharides and Plant Cell Walls
Cornell University
2021-2025
Plant (United States)
2023-2025
University of California, Berkeley
2016-2024
Energy Biosciences Institute
2016-2021
Harvard University
2013-2014
Center for Systems Biology
2013-2014
Filamentous fungi, such as Neurospora crassa , are very efficient in deconstructing plant biomass by the secretion of an arsenal cell wall-degrading enzymes, remodeling metabolism to accommodate production secreted and enabling transport intracellular utilization components. Although a number enzymes transcriptional regulators involved have been identified, how filamentous fungi sense integrate nutritional information encoded wall into regulatory hierarchy for optimal complex carbon sources...
Significance Microbes have evolved complex signaling networks to identify and prioritize utilization of available energy sources. For many fungi, such as Neurospora crassa , this entails distinguishing between an array carbon sources, including insoluble carbohydrates in plant cell walls. Here, we identified a repressor the cellulose-response pathway N . Using derepressed mutant, implicated conserved hyperosmotic-response MAP kinase regulating response carbohydrates. We hypothesize that...
ABSTRACT Lori Huberman works in the field of fungal genetics, with an emphasis on investigating genetic mechanisms fungi use to sense and respond nutrients toxins their environment. In this mSphere Influence article, she reflects how “Rapid quantification mutant fitness diverse bacteria by sequencing randomly bar-coded transposons” K. M. Wetmore, N. Price, R. J. Waters, S. Lamson, et al. (mBio 6:e00306-15, 2015, https://doi.org/10.1128/mBio.00306-15 ) made impact her establishing...
Cells must accurately sense and respond to nutrients compete for resources establish growth. Phosphate is a critical nutrient source necessary signaling, energy metabolism, synthesis of nucleic acids, phospholipids, cellular metabolites. During phosphate limitation, fungi import from the environment liberate phosphate-containing molecules in cell. In model filamentous fungus Neurospora crassa, starvation response regulated by conserved transcription factor NUC-1. The activity NUC-1 repressed...
ABSTRACT Cells must sense and respond to available nutrients utilize resources establish fungal colonies. Saprophytic microbes harvest carbon from plant biomass, which contains insoluble, complex carbohydrates that be degraded extracellularly prior import into the cell. Filamentous fungi can degrade these carbohydrates. However, while many single cellular building blocks of insoluble polysaccharides, they frequently are unable Cellobiose is a disaccharide breakdown product cellulose, most...
Significance Microorganisms have evolved transcriptional networks to prioritize utilization of available nutrient sources. For filamentous fungi, such as Neurospora crassa , this entails distinguishing between a variety organic and inorganic nitrogen Here, we transcriptionally profiled the response N. sources used DNA affinity purification sequencing characterize role regulatory genes their direct downstream targets. We identified transcription factor responsible for regulating involved in...
Abstract Haploid budding yeast has two mating types, defined by the alleles of MAT locus, MATa and MATα. Two haploid cells opposite types mate signaling to each other using reciprocal pheromones receptors, polarizing growing toward other, eventually fusing form a single diploid cell. The receptors are necessary sufficient define type, but mating-type-specific proteins make more efficient. We examined role these genetically engineering “transvestite” that swap pheromone, pheromone receptor,...
Mating of the budding yeast, Saccharomyces cerevisiae, occurs when two haploid cells opposite mating types signal using reciprocal pheromones and receptors, grow towards each other, fuse to form a single diploid cell. To fuse, both dissolve their cell walls at point contact. This event must be carefully controlled because osmotic pressure differential between cytoplasm extracellular environment causes with unprotected plasma membranes lyse. If wall-degrading enzymes diffuse through wall,...
Accurate nutrient sensing is important for rapid fungal growth and exploitation of available resources. Sulfur an source found in a number biological macromolecules, including proteins lipids. The model filamentous fungus Neurospora crassa capable utilizing sulfur variety sources from amino acids to sulfate. During starvation, the transcription factor CYS-3 responsible upregulation genes involved uptake assimilation. Using combination RNA sequencing DNA affinity purification sequencing, we...
Fungi are responsible for diseases that result in the deaths of over a million individuals each year and devastating crop infestations threaten global food supplies. However, outside select few model organisms, majority fungal genes uncharacterized. The roles these biology organism, pathogenesis, mediating interactions with environment other microbes unknown. Historically, gene characterization has primarily relied on classical genetic screens. advances sequencing technology have enabled...
ABSTRACT Filamentous fungi are important producers of enzymes and secondary metabolites. The industrial thermophilic species, Thermothelomyces thermophilus, is closely related to the model fungus, Neurospora crassa . A critical aspect filamentous fungal life cycle production asexual spores (conidia), which regulated by various stimuli, including nutrient availability. Several species fungi, T. thermophilus , produce conidia under submerged fermentation conditions, can be detrimental product...
Accurate nutrient sensing is important for rapid fungal growth and exploitation of available resources. Sulfur an source found in a number biological macromolecules, including proteins lipids. The model filamentous fungus Neurospora crassa capable utilizing sulfur variety sources from amino acids to sulfate. During starvation, the transcription factor CYS-3 responsible upregulation genes involved uptake assimilation. Using combination RNA sequencing DNA affinity purification sequencing, we...