A5101758353- RNA modifications and cancer
- Anesthesia and Neurotoxicity Research
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Atherosclerosis and Cardiovascular Diseases
- Cancer-related gene regulation
- Single-cell and spatial transcriptomics
- Anesthesia and Sedative Agents
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Endoplasmic Reticulum Stress and Disease
- Neonatal and fetal brain pathology
- Autophagy in Disease and Therapy
- Electrochemical Analysis and Applications
- Environmental Toxicology and Ecotoxicology
- Sirtuins and Resveratrol in Medicine
- Cancer, Stress, Anesthesia, and Immune Response
- Signaling Pathways in Disease
- Geology and Paleoclimatology Research
- Mitochondrial Function and Pathology
- Cancer Research and Treatments
- Cardiac Fibrosis and Remodeling
- Methane Hydrates and Related Phenomena
- Heme Oxygenase-1 and Carbon Monoxide
- Neuroinflammation and Neurodegeneration Mechanisms
Hainan University
2024
First Affiliated Hospital of Zhengzhou University
2014-2024
Chinese Academy of Sciences
2022-2024
Shanghai Institute of Materia Medica
2022-2024
Chinese PLA General Hospital
2024
Sanya University
2024
Xiamen University
2008-2023
Fourth Hospital of Hebei Medical University
2020-2022
Hebei Medical University
2020-2022
Tianjin Medical University
2022
Abstract Background : Recent reports suggested the involvement of oxidative stress- and endoplasmic reticulum stress (ERS)-associated pathways in progression ischemia/reperfusion (I/R) injury. Notoginsenoside R1 (NGR1) is a novel saponin isolated from P. notoginseng , which has history prevention treatment cardiovascular diseases. Objective We aimed to examine cardioprotective effects NGR1 on I/R-induced heart dysfunction ex vivo vitro. Methods H9c2 cadiomyocytes were incubated with for 24 h...
Abstract Background Red blood cells (RBCs) transfusion is related to perioperative neurocognitive disorders. The toxic effect of free heme has been identified in many pathologies. However, the underlying mechanisms RBCs or cognitive impairment have not clearly explored. Therefore, this research was conducted determine mechanism heme-induced neuroinflammation and impairment. Methods Rats were received intraperitoneal injection hemin alone combined with intracerebroventricular Hemopexin (HPX),...
Abstract Background Hepatocellular carcinoma (HCC) is one of the leading causes cancer‐related deaths globally. Herein, we explored underlying mechanism by which Propofol inhibited development HCC. Methods 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay was carried out to detect viability and proliferation. Quantitative real‐time polymerase chain reaction (qRT‐PCR) Western blot were performed expression long noncoding RNA (lncRNA) H19, microRNA‐520a‐3p (miR‐520a‐3p),...
N6-methyladenosine (m6A) is the most abundant methylation modification on mRNA in mammals. Fat mass and obesity-related protein (FTO) main RNA m6A demethylase. FTO involved occurrence maintenance of neuropathic pain (NP). NP often induces mental disorders. We found that downregulated expression anterior cingulate cortex (ACC), inhibited matrix metalloproteinase-9 (MMP-9) ACC, maladjusted brain-derived neurotrophic factor precursor (proBDNF) mature (mBDNF) levels induced anxiety-...
We described Cilo-seq for high-performance scRNA-seq library construction in a single device with programmed and addressable droplet handling based on digital microfluidics, which allowed simultaneously convenient single-cell isolation, efficient nucleic acid amplification, low-loss purification high-quality preparation.
Sleep is essential for the body's repair and recovery, including supplementation with antioxidants to maintain balance of redox state. Changes in sleep patterns have been reported alter this function, leading changes disease susceptibility or behavior. Here, we recruited healthy male physicians measured extent effect overnight deprivation (SD) recovery (RS) on nociceptive thresholds systemic (plasma-derived) metabolism, namely, major glutathione (GSH), catalase (CAT), malondialdehyde (MDA),...
Propofol has been identified to perform anti-tumor functions in glioma. However, the molecular mechanisms underlying propofol-induced prevention on migration and invasion of glioma cells remain unclear.Cell proliferation, were measured by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay transwell assay, respectively. The expression microRNA (miR)-206 Rho-associated coiled coil-containing protein kinase 1 (ROCK1) was detected quantitative real-time polymerase chain...
Myocardial infarction (MI) is a vital cause of death and disability globally. The primary treatment for diminishing acute myocardial ischemic injury reperfusion, which may induce cardiomyocyte death. Our aim to unravel the mechanism sevoflurane (Sev) in microRNA-219a (miR-219a)-mediated regulation absent melanoma 2 (AIM2) TLR4/MyD88 pathway during ischemia/reperfusion (I/R). area MI apoptosis cardiomyocytes developed mouse model were evaluated by TTC staining TUNEL, respectively. After...
The aim of our study was to explore the relationships between M2 isoform pyruvate kinase (PKM2) and sensitivity human non-small cell lung cancer (NSCLC) cells docetaxel in vitro.With method plasmid transfection, we silenced expression PKM2 successfully A549 H460 cells. Western blotting real-time PCR were applied detect at protein gene levels. Cell viability examined by CCK8 assay. cycle distribution apoptosis flow cytometry. P21 Bax detected.Expression mRNA significantly decreased shRNA...
Abstract Single‐cell microRNA (miRNA) sequencing has allowed for comprehensively studying the abundance and complex networks of miRNAs, which provides insights beyond single‐cell heterogeneity into dynamic regulation cellular events. Current benchtop‐based technologies miRNA are low throughput, limited reaction efficiency, tedious manual operations, high reagent costs. Here, a highly multiplexed, efficient, integrated, automated sample preparation platform is introduced based on digital...
Traumatic brain injury (TBI) is characterized by physical damage to the tissues, ensuing transitory or permanent neurological dysfunction featured with neuronal loss and subsequent damage. Sevoflurane, a widely used halogenated anesthetic in clinical settings, has been reported alleviate neuron apoptosis TBI. Nevertheless, underlying mechanism behind this alleviation remains unknown, thus was focus of current study. First, Feeney models were established induce TBI rats. Subsequently,...
The impact of mitochondrial dysfunction on the pathogenesis cardiovascular disease is increasing. However, precise underlying mechanism remains unclear. Mitochondria produce cellular energy through oxidative phosphorylation while regulating calcium homeostasis, respiration, and production biosynthetic chemicals. Nevertheless, problems related to cardiac metabolism, defective proteins, mitophagy, structural changes in membranes can cause diseases via dysfunction. Mitofilin a critical inner...
Gastric cancer is one of the most common cancers and considered 5 th frequent occurring worldwide. It has gained great attention from clinicians researchers because high mortality rate. generally treated with chemotherapy, radiotherapy, surgery. Recently, additional treatment options including immunotherapy targeted therapy have been developed. However, poor prognosis, limited survival rate patients, drug resistance to remain critical problems. To improve or overcome bottleneck treatment,...
Restenosis is a major limiting factor for successful outcome in type 2 diabetes (T2D) patients undergoing percutaneous coronary intervention (PCI). The aim of this study to explore the role and regulatory mechanism FOS-like antigen 1 (FOSL1) restenosis T2D. A T2D with mouse model was established by combination high-fat diet streptozotocin injection wire-injury. High glucose (HG)-treated vascular smooth muscle cells (VSMCs) were used mimic vitro. results quantitative real time PCR western...