A5040492527- Dark Matter and Cosmic Phenomena
- Astrophysics and Cosmic Phenomena
- Radiation Therapy and Dosimetry
- Particle Detector Development and Performance
- Solar and Space Plasma Dynamics
- Particle physics theoretical and experimental studies
- Neutrino Physics Research
- Plant Virus Research Studies
- High-Energy Particle Collisions Research
- Legume Nitrogen Fixing Symbiosis
- Radiation Detection and Scintillator Technologies
- Nuclear Physics and Applications
- Radiation Effects in Electronics
- Ionosphere and magnetosphere dynamics
- Agricultural pest management studies
- Graphite, nuclear technology, radiation studies
- Plant tissue culture and regeneration
- Quantum Chromodynamics and Particle Interactions
- Nuclear physics research studies
- Atmospheric Ozone and Climate
- Radioactivity and Radon Measurements
- Atomic and Subatomic Physics Research
- Gamma-ray bursts and supernovae
- Transgenic Plants and Applications
- Electron and X-Ray Spectroscopy Techniques
University of Alabama in Huntsville
1983-2024
University of Tennessee at Chattanooga
2020-2024
Institute of Experimental Physics of the Slovak Academy of Sciences
2023
University of Tübingen
2018
Université Paris-Sud
2017
Institut National de Physique Nucléaire et de Physique des Particules
2017
Centre National de la Recherche Scientifique
2017
Marshall Space Flight Center
1998-2013
John Innes Centre
1978-2008
California Institute of Technology
1997
The results of a test general relativity with use hydrogen-maser frequency standard in spacecraft launched nearly vertically upward to 10 000 km are reported. agreement the observed relativistic shift prediction is at 70\ifmmode\times\else\texttimes\fi{}${10}^{\ensuremath{-}6}$ level.
We have studied the F-actin network in cycling suspension culture cells of carrot (Daucus carota L.) using rhodaminyl lysine phallotoxin (RLP). In addition to conventional fixation with formaldehyde, we used two different nonfixation methods before adding RLP: extracting a stabilizing buffer; inducing transient pores plasma membrane pulses direct current (electroporation). These alternative for introducing RLP revealed additional features actin not seen aldehyde-fixed cells. The...
Measurements of the cosmic-ray hydrogen and helium spectra at energies from 20 to 800 TeV are presented. The experiments were performed on a series twelve balloon flights, including several long duration Australia South America Antarctic circumpolar flights. No clear evidence is seen for spectral break. Both consistent with power laws over entire energy range, integral indices 1.80 ± 0.04 1.68+ 0.04−0.06 protons helium, respectively. results fully expectations based supernova shock...
We have investigated the ability of African cassava mosaic virus DNA A mutants, containing disrupted complementary-sense genes, to infect Nicotiana benthamiana and replicate in tabacum protoplasts. Three overlapping open reading frames (ORFs) with capacity encode proteins an Mr greater than 10K (AC1, AC2 AC3) are highly conserved between geminiviruses that dicotyledonous plants one (AC4) is less well conserved. Of these, only AC1 a prerequisite for replication; disruption this ORF rendered...
SUMMARY Mutants of African cassava mosaic virus containing extensive deletions across the coat protein gene that remove up to one-third genomic component have been constructed and shown be infectious when mechanically inoculated onto Nicotiana benthamiana by leaf abrasion. Using N. tabacum protoplasts we demonstrate mutant pCLV. CPΔ11, a 712 bp deletion, is competent for replication in its deleted form. However, systemic spread CPΔ11 other deletion mutants associated with reversion DNA 1...
SUMMARY Tobacco protoplasts were infected with cowpea chlorotic mottle virus and its RNA in the presence of poly-l-ornithine to yield 106 107 particles per protoplast representing a probable increase concentration at least 1000-fold by 24 72 h after inoculation. The optimum inoculum input for was about 0.5 µg/ml 1.2 µg/ml; 60 7%, respectively, became infected, as judged fluorescent antibody staining. Data relating growth curves, concentrations, pH effects are presented along electron...
A simple method is described for the electrofusion of plant protoplasts. Protoplasts were aggregated in a radio-frequency field (10 V RMS, 0.5 MHz) 15–30 s with an inter-electrode distance J mm. They then fused 300-V DC pulse. The protoplasts able to divide after this treatment. trans-ferrable electrode permitted l-ml volumes culture standard tissue-culture dishes about 20 s.
view Abstract Citations (76) References (30) Co-Reads Similar Papers Volume Content Graphics Metrics Export Citation NASA/ADS Energy Spectra of Cosmic Rays above 1 TeV per Nucleon Burnett, T. H. ; Dake, S. Derrickson, J. Fountain, W. F. Fuki, M. Gregory, C. Hayashi, Holynski, R. Iwai, Jones, V. Jurak, A. Lord, Miyamura, O. Oda, Ogata, Parnell, Roberts, E. Strausz, Tabuki, Takahashi, Y. Tominaga, Watts, Wefel, P. Wilczynska, B. Wilczynski, Wilkes, Wolter, Wosiek, JACEE Collaboration Direct...
We describe a tool suite, CRÈME, which combines existing capabilities of CREME96 and CREME86 with new radiation environment models Monte Carlo computational for single event effects total ionizing dose.
Problems encountered in the preparation and culture of tobacco leaf protoplasts are discussed. The conditions under which plants were cultivated was important; only well-grown a reliable source protoplasts. age also critical; present work leaves from White Burley aged 40–60 days found to give very reproducible results. Plants older or younger than this unsatisfactory. Protoplasts required higher levels auxin during did those other varieties examined {Xanthi Xanthi NC) level initially induce...
Energy spectra of protons and helium nuclei in the primary cosmic rays were measured above 1 TeV a series balloon flights emulsion chambers. Differential may be represented by power laws indices -2.81\ifmmode\pm\else\textpm\fi{}0.13 -2.83\ifmmode\pm\else\textpm\fi{}0.20 for He, respectively. No index change was observed either species over energy ranges 5-500 2-50 TeV/nucleon He. Intensities consistent with extrapolations previously published data below TeV/nucleon.
The highly anisotropic nature of the radiation in low‐Earth orbit has been ignored for most spacecraft shielding calculations made to date because standard environmental models describe omnidirectional flux only, varying attitude environment is assumed average out effect and added complexity calculation. Space Station planned be stabilized with respect velocity vector local vertical. Thus it will pass through South Atlantic Anomaly where encountered much same on each pass. Any calculation...
SUMMARY Neither tobacco mesophyll protoplasts nor leaves could be infected with wild type brome mosaic virus (BMV wt) or its RNA, but both were when inoculated 50 µg/ml more of a variant BMV V5). This is about 100 × the amount required for efficient infection by cowpea chlorotic mottle (CCMV). Poly-l-ornithine stimulated V5, was not essential. V5. RNA inoculum caused under conditions similar to those needed their CCMV-RNA.
SUMMARY The susceptibility of tobacco protoplasts to infection with cowpea chlorotic mottle virus varied the age both plants and leaves used as a source for protoplasts. efficiency freshly prepared stored could be increased by an improved direct inoculation procedure. Poly-l-ornithine, poly-l-arginine, poly-l-lysine poly-d-lysine were all effective in inducing infection, first stage which may occur within 20 s exposure inoculum. effects temperature, injury sodium azide on suggested that...