To assess whether Fourier Transform Infrared (FTIR) spectroscopy could be used to evaluate and monitor lipid extraction processes, the methods of Folch, Bligh Lewis were used. Biomass oleaginous fungi Mucor circinelloides Mortierella alpina employed as lipid-rich material for extraction. The presence lipids was determined by recording infrared spectra all components in procedure, such biomass before after extraction, water extract phases. revealed incomplete three applied M.circinelloides it...

Oleaginous yeasts are considered as a potential lipid source for food, feed and biofuel production. In order to make the yeast-based production environmentally economically sustainable, there is need screening studies in find best yeast producers on different substrates, optimize cultivation conditions. Since target parameter of such amounts profiles, an analytical technique that able perform analyses rapidly, reproducible with high precision highly desirable. The main objective this study...

Infrared spectroscopy hampered by physical distortions from scattering and instrumental effects. Therefore, it is generally accepted that spectra should be pre-processed before further data analysis. Deep learning community offers augmentation techniques as an alternative approach to deal with variability in the data. In this paper we propose Extended Multiplicative Signal Augmentation (EMSA) method for augmenting infrared spectra. order study effect of pre-processing combine them a wide...

Microbial oils, generated from lignocellulosic material, have great potential as renewable and sustainable alternatives to fossil-based fuels chemicals. By unravelling the diversity of lipid accumulation physiology in different oleaginous yeasts grown on various carbon sources present lignocellulose hydrolysate (LH), new targets for optimisation can be identified. Monitoring formation over time is essential understanding physiology. This study investigated a variety ascomycetous...

Fungal production of polyunsaturated fatty acids (PUFAs) is a highly potential approach in biotechnology. Currently the main focus directed towards screening hundreds strains order to select few ones. Thus, reliable method for high number within short period time needed. Here, we present novel PUFA-producing fungi by high-throughput microcultivation and FTIR spectroscopy. In study selected Mucor were grown media with different carbon sources acid profiles predicted on basis obtained spectral...

Oleaginous fungi can accumulate lipids by utilizing a wide range of waste substrates. They are an important source for the industrial production omega-6 polyunsaturated fatty acids (gamma-linolenic and arachidonic acid) have been suggested as alternative route biodiesel production. Initial research steps various applications include screening in order to find efficient fungal producers with desired acid composition. Traditional cultivation methods (shake flask) lipid analysis (extraction-gas...

Mucoromycota fungi are important producers of low- and high-value lipids. Mortierella alpina is used for arachidonic acid production at industrial scale. In addition, oleaginous promising candidates biodiesel production. A critical step in the development such biotechnological applications selection suitable strains lipid The aim present study was to use Duetz-microtiter plate system combined with Fourier transform infrared (FTIR) spectroscopy high-throughput screening potential 100 produce...

Analyses of substrate and metabolites are often bottleneck activities in high-throughput screening microbial bioprocesses. We have assessed Fourier transform infrared spectroscopy (FTIR), combination with high throughput micro-bioreactors multivariate statistical analyses, for analysis In our previous study, we demonstrated that (HTS) FTIR can be used estimating content composition intracellular metabolites, namely triglyceride accumulation oleaginous filamentous fungi. As a continuation...

Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols organic acids. However, far commercialisation is concerned, establishing industrial biotechnological processes based on still challenging due to the high costs compared final product value. Therefore, development co-production concept highly desired since more than one valuable could be...

Journal Article Characterization of food spoilage fungi by FTIR spectroscopy Get access V. Shapaval, Shapaval Nofima AS Ås NorwayCentre for Integrative Genetics (CIGENE), Department Mathematical Sciences and Technology Norwegian University Life Norway Correspondence Volha AS, Centre Biospectroscopy Data modeling, Osloveien 1, N‐1430 Ås, Norway. E‐mail: volha.shapaval@nofima.no Search other works this author on: Oxford Academic Google Scholar J. Schmitt, Schmitt Synthon GmbH Heidelberg...

Abstract Oleaginous filamentous fungi grown under the nitrogen limitation, accumulate high amounts of lipids in form triacylglycerides (TAGs) with fatty acid profiles similar to plant and fish oils. In this study, we investigate effect six phosphorus source concentrations combined two types substrate (yeast extract ammonium sulphate), on biomass formation, lipid production, profile for nine oleaginous Mucoromycota fungi. The analysis was performed by gas chromatography flame ionization...

Mucoromycota fungi are promising for the production of second-generation biofuel from single-cell oils (SCOs) using lignocellulose biomass. Despite lack enzymatic capability efficiently degrading in fungi, simultaneous saccharification and fermentation (SSF) offers an attractive solution by combining hydrolysis same procedure. This study explored specific traits various species to evaluate their suitability SSF, due frequent significant gap between microorganism enzyme optimal conditions....

Abstract Characterization and identification of fungi in food industry is an important issue both for routine analysis trouble‐shooting incidences. Present microbial techniques fungal characterization suffer from a low throughput are time consuming. In this study we present protocol high‐throughput microcultivation spectral by Fourier transform infrared spectroscopy. For the 11 species total five different genera ( Alternaria , Aspergillus Mucor Paecilomyces Phoma ) were analyzed FTIR All...

Carotenogenic yeasts are non-conventional oleaginous microorganisms capable of utilizing various waste substrates. In this work, four red yeast strains (Rhodotorula, Cystofilobasidium, and Sporobolomyces sp.) were cultivated in media containing crude, emulsified, enzymatically hydrolyzed animal fat, compared with glucose glycerol, as single C-sources. Cell morphology (cryo-SEM (cryo-scanning electron microscopy), TEM (transmission microscopy)), production biomass, lipase, biosurfactants,...

Abstract Background Oleaginous filamentous fungi can accumulate large amount of cellular lipids and potentially serve as a major source oleochemicals for food, feed, chemical, pharmaceutical, transport industries. Transesterification microbial oils is an essential step in lipid production at both laboratory industrial scale. Direct transesterification considerably reduce costs, increase sample throughput improve yields (in particular fatty acid methyl esters, FAMEs). There need the...

Single-channel optical density measurements of population growth are the dominant large scale phenotyping methodology for bridging gene-function gap in yeast. However, a substantial amount genetic variation induced by single allele, gene or double knock-out technologies fail to manifest detectable phenotypes under conditions readily testable laboratory. Thus, new high-throughput capable providing information about molecular level consequences sorely needed. Here we report protocol Fourier...

Recent developments in molecular biology and metabolic engineering have resulted a large increase the number of strains that need to be tested, positioning high-throughput screening microorganisms as an important step bioprocess development. Scalability is crucial for performing reliable microorganisms. Most scalability studies from microplate systems controlled stirred-tank bioreactors been performed so far with unicellular We compared cultivation industrially relevant oleaginous...

The biomass of Mucor circinelloides, a dimorphic oleaginous filamentous fungus, has significant nutritional value and can be used for single cell oil production. Metal ions are micronutrients supporting fungal growth metabolic activity cellular processes. We investigated the effect 140 different substrates, with varying amounts metal phosphate concentration, on growth, chemistry, lipid accumulation, profile M. circinelloides. A high-throughput set-up consisting Duetz microcultivation system...

Oleaginous filamentous fungi can accumulate large amount of cellular lipids and biopolymers pigments potentially serve as a major source biochemicals for food, feed, chemical, pharmaceutical, transport industries. We assessed suitability Fourier transform (FT) Raman spectroscopy screening process monitoring in biotechnology. Six Mucoromycota strains were cultivated microbioreactors under six growth conditions (three phosphate concentrations the presence absence calcium). FT-Raman FT-infrared...

High-throughput screening (HTS) methods for characterization of microbial production polyhydroxyalkanoates (PHA) are currently under investigated, despite the advent such systems in related fields. In this study, phenotypic microarray by Biolog PM1 Halomonas sp. R5-57 and Pseudomonas MR4-99 identified 49 54 carbon substrates to be metabolized these bacteria, respectively. Growth on 15 ( R5-57) 14 MR4-99) was subsequently characterized 96-well plates using medium with low nitrogen...

In this study lignocellulosic sugars from Norway spruce were used for production of docosahexaenoic acid (DHA) by the marine thraustochytrid Aurantiochytrium limacinum SR21. Enzymatically prepared hydrolysate was combined with a complex nitrogen source and different amounts salts. Shake flask batch cultivations revealed that addition extra salts not needed optimal growth. Upscaling to fed-batch bioreactors yielded up 55 g/L cell dry mass total fatty content 44% (w/w) out which 1/3 DHA....

The rapid and accurate identification of pathogen yeast species is crucial for clinical diagnosis due to the high level mortality morbidity induced, even after antifungal therapy. For this purpose, new rapid, high-throughput reliable methods are required. In work we described a combined approach based on two techniques in order improve pathogenic strains. Next Generation Sequencing (NGS) ITS D1/D2 LSU marker regions together with FTIR spectroscopy were applied identify 256 strains belonging...