Abstract MALDI time-of-flight mass spectrometry (MALDI-TOF MS) has become a widely used tool for the classification of biological samples. The complex chemical composition pollen grains leads to highly specific, fingerprint-like spectra, with respect species. Beyond species-specific composition, variances in chemistry can be hierarchically structured, including level different populations, environmental conditions or genotypes. We demonstrate here sensitivity MALDI-TOF MS regarding adaption...

Calculating derivatives of spectral data by the Savitzky–Golay (SG) numerical algorithm is often used as a preliminary preprocessing step to resolve overlapping signals, enhance signal properties, and suppress unwanted features that arise due nonideal instrument sample properties. Addressing these issues, study simulated measured infrared partial least-squares regression has been conducted. The sets were modeled considering range undesired chemical physical anomalies variations can occur in...

Immunoglobulin G (IgG) crystallizable fragment (Fc) glycosylation is crucial for antibody effector functions, such as antibody-dependent cell-mediated cytotoxicity, and their pharmacokinetic pharmacodynamics behavior. To monitor the Fc-glycosylation in bioprocess development, well product characterization release analytics, reliable techniques analysis are needed. A wide range of analytical methods has found its way into these applications. In this study, a comprehensive comparison was...

To assess whether Fourier Transform Infrared (FTIR) spectroscopy could be used to evaluate and monitor lipid extraction processes, the methods of Folch, Bligh Lewis were used. Biomass oleaginous fungi Mucor circinelloides Mortierella alpina employed as lipid-rich material for extraction. The presence lipids was determined by recording infrared spectra all components in procedure, such biomass before after extraction, water extract phases. revealed incomplete three applied M.circinelloides it...

The degradation of proteins by asparagine deamidation and aspartate isomerization is one several chemical pathways for recombinant antibodies. In this study, we have identified two solvent accessible sites (light chain aspartate-56 heavy aspartate-99/101) in the complementary-determining regions a IgG1 antibody susceptible to under elevated temperature conditions. For both hot-spots, degree was found be significantly higher than asparagine-(387, 392, 393) conserved CH3 region, which has been...

Pollen studies are important for the assessment of present and past environment, including biodiversity, sexual reproduction plants plant-pollinator interactions, monitoring aeroallergens, impact climate pollution on wild communities cultivated crops. Although information chemical composition pollen is importance in all those research areas, chemistry has been rarely measured due to complex time-consuming analyses. Vibrational spectroscopies, coupled with multivariate data analysis, have...

Background It is imperative to have reliable and timely methodologies for analysis monitoring of seed plants in order determine climate-related plant processes. Moreover, impact environment on fitness predominantly based studies female functions, while the contribution male gametophytes mostly ignored due missing data pollen quality. We explored use infrared spectroscopy an inexpensive rapid characterization plants. Methodology The study was measurement samples by two Fourier transform...

Oleaginous fungi can accumulate lipids by utilizing a wide range of waste substrates. They are an important source for the industrial production omega-6 polyunsaturated fatty acids (gamma-linolenic and arachidonic acid) have been suggested as alternative route biodiesel production. Initial research steps various applications include screening in order to find efficient fungal producers with desired acid composition. Traditional cultivation methods (shake flask) lipid analysis (extraction-gas...

Mucoromycota fungi are important producers of low- and high-value lipids. Mortierella alpina is used for arachidonic acid production at industrial scale. In addition, oleaginous promising candidates biodiesel production. A critical step in the development such biotechnological applications selection suitable strains lipid The aim present study was to use Duetz-microtiter plate system combined with Fourier transform infrared (FTIR) spectroscopy high-throughput screening potential 100 produce...

Background Analysis of pollen grains reveals valuable information on biology, ecology, forensics, climate change, insect migration, food sources and aeroallergens. Vibrational (infrared Raman) spectroscopies offer chemical characterization via identifiable spectral features without any sample pretreatment. We have compared the level that can be obtained by different multiscale vibrational spectroscopic techniques. Methodology Pollen from 15 species Pinales (conifers) were measured seven...

Analyses of substrate and metabolites are often bottleneck activities in high-throughput screening microbial bioprocesses. We have assessed Fourier transform infrared spectroscopy (FTIR), combination with high throughput micro-bioreactors multivariate statistical analyses, for analysis In our previous study, we demonstrated that (HTS) FTIR can be used estimating content composition intracellular metabolites, namely triglyceride accumulation oleaginous filamentous fungi. As a continuation...

The quality control testing of chemical degradations in the bio-pharmaceutical industry is currently under controversial debate. Here we have systematically applied vitro and vivo stress conditions to investigate influence protein degradation on structure-function. Extensive purification characterization enabled identification functional assessment physiological modification sites variable complementarity-determining regions (CDRs) conserved region trastuzumab. We demonstrate that...

Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols organic acids. However, far commercialisation is concerned, establishing industrial biotechnological processes based on still challenging due to the high costs compared final product value. Therefore, development co-production concept highly desired since more than one valuable could be...

The EF(0)F(1)-ATP synthase mutants bQ64C and gamma T106C were labelled selectively with the fluorophores tetramethylrhodamine (TMR) at b-subunit a cyanine (Cy5) gamma-subunit. After reconstitution into liposomes, these double-labelled enzymes catalyzed ATP synthesis rate of 33 s(-1). Fluorescence TMR Cy5 was measured confocal set-up for single-molecule detection. Photon bursts detected, when liposomes containing one enzyme traversed volume. Three states different fluorescence resonance...

Classification, discrimination, and biochemical assignment of vibrational spectra pollen samples belonging to 43 different species the order Pinales has been made using three techniques. The comparative study transmission (KBr pellet) attenuated total reflection (ATR) Fourier transform infrared (FT-IR) FT-Raman spectroscopies was based on substantial variability grain size, shape, relative composition. Depending penetration depth probe light, techniques acquire predominant information either...

Abstract Oleaginous filamentous fungi grown under the nitrogen limitation, accumulate high amounts of lipids in form triacylglycerides (TAGs) with fatty acid profiles similar to plant and fish oils. In this study, we investigate effect six phosphorus source concentrations combined two types substrate (yeast extract ammonium sulphate), on biomass formation, lipid production, profile for nine oleaginous Mucoromycota fungi. The analysis was performed by gas chromatography flame ionization...

Microbial pigments can replace synthetic organic which often produced in unsustainable way and be toxic. Therefore, search for new pigment producing microorganisms is of high interest industry. In this study, a screening characterization profile photostability seventy-four newly isolated Antarctic bacteria using Fourier-transform (FT) Raman spectroscopy HPLC-MS was performed. Screening the bacterial biomass by FT-Raman identified thirty-seven strains from genera Agrococcus, Arthrobacter,...

Mucoromycota fungi are promising for the production of second-generation biofuel from single-cell oils (SCOs) using lignocellulose biomass. Despite lack enzymatic capability efficiently degrading in fungi, simultaneous saccharification and fermentation (SSF) offers an attractive solution by combining hydrolysis same procedure. This study explored specific traits various species to evaluate their suitability SSF, due frequent significant gap between microorganism enzyme optimal conditions....

A new bichromophoric dyad based on an alkyl-functionalized aminonaphthalimide as energy-donor chromophore and [Ru(dcbpy)2(acac)]Cl (dcbpy=4,4'-dicarboxybipyridine, acac=acetylacetonato) energy acceptor sensitizing is synthesized. Efficient quenching of the donor-chromophore emission observed in solution, presumably due to resonant transfer. This then used a sensitizer dye solar cell. By comparing spectral properties transparent cells sensitized with [Ru(dcbpy)2(acac)]Cl, it possible...

Summary Fourier transform infrared (FTIR) spectroscopy enables chemical analysis of pollen samples for plant phenotyping to study plant–environment interactions, such as influence climate change or pathogens. However, current approach, microspectroscopy and attenuated total reflection spectroscopy, does not allow high‐throughput protocols. This at hand suggests a new spectroscopic method characterization pollen. Samples were measured thin films fragments using Bruker FTIR spectrometer with...