A5039322669- Axon Guidance and Neuronal Signaling
- Neurological Disease Mechanisms and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disorders and Treatments
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- COVID-19 and healthcare impacts
- Long-Term Effects of COVID-19
- Barrier Structure and Function Studies
- Genetics and Neurodevelopmental Disorders
- Flavonoids in Medical Research
- COVID-19 Clinical Research Studies
- 3D Printing in Biomedical Research
- Spinal Cord Injury Research
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
- Plant-based Medicinal Research
University of Miami
2014-2022
University of Edinburgh
2011-2014
MRC Centre for Regenerative Medicine
2011-2013
Methylation of cytosine is a DNA modification associated with gene repression. Recently, novel modification, 5-hydroxymethylcytosine (5-hmC) has been discovered. Here we examine 5-hmC distribution during mammalian development and in cellular systems, show that the developmental dynamics are different from those 5-methylcytosine (5-mC); particular enriched embryonic contexts compared to adult tissues. A detectable signal appears pre-implantation starting at zygote stage, where paternal genome...
Cultures of human embryonic stem cell typically rely on protein matrices or feeder cells to support attachment and growth, while mechanical, enzymatic chemical dissociation methods are used for cellular passaging. However, these ill defined, thus introducing variability into the system, may damage cells. They also exert selective pressures favouring aneuploidy loss differentiation potential. Here we report identification a family chemically defined thermoresponsive synthetic hydrogels based...
Abstract Damage to the cerebrovascular network is a major contributor dysfunction in patients suffering from traumatic brain injury (TBI). Vessels are composed of lumen-forming endothelial cells that associate closely with both glial and neuronal units establish functional blood–brain barrier (BBB). Under normal physiological conditions, these vascular play important roles central nervous system (CNS) homeostasis by delivering oxygen nutrients while filtering out molecules could be harmful;...
Abstract We demonstrate that EphB3 receptors mediate oligodendrocyte (OL) cell death in the injured spinal cord through dependence receptor mechanism. OLs adult express as well other members of Eph family. Spinal injury (SCI) is associated with tissue damage, cellular loss and disturbances EphB3-ephrinB3 protein balance acutely (days) after initial impact creating an environment for a receptor-mediated to occur. Genetic ablation promotes OL survival increased expression myelin basic improved...
Cerebrovascular injuries, such as stroke and other vascular insults to the brain, present significant challenges neurological function recovery. Within complex realm of epigenetic modifications, hydroxymethylation emerges a crucial factor in orchestrating mechanisms for neuroprotection repair following injuries. This review consolidates current research on multifaceted role 5-hydroxymethylcytosine (5hmC) regulating molecular cellular processes vital neuronal survival regeneration after...
Abstract Clinical trials examining neuroprotective strategies after brain injury, including those targeting cell death mechanisms, have been underwhelming. This may be in part due to an incomplete understanding of the signalling mechanisms that induce traumatic injury. The recent identification a new family receptors initiate pro-cell signals absence their ligand, called dependence receptors, provides insight into factors contribute Here, we show blocking receptor EphB3 improves...
Cerebrovascular disorders such as stroke and intracerebral hemorrhage (ICH) occur in COVID-19 patients with pre-existing hypertension. Elevated blood pressure increases the risk of COVID-19-related morbidity mortality. Although, exact mechanisms action are not well understood, imbalance renin-angiotensin-aldosterone system (RAAS), hypertension-induced cerebrovascular abnormalities can facilitate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infiltration into central nervous...