A5067425254- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Nerve injury and regeneration
- Neuroscience and Neuropharmacology Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Spinal Cord Injury Research
- Pharmacological Receptor Mechanisms and Effects
- Neuropeptides and Animal Physiology
- Amino Acid Enzymes and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Zebrafish Biomedical Research Applications
- Biochemical effects in animals
- Anesthesia and Neurotoxicity Research
- Angiogenesis and VEGF in Cancer
- Pain Mechanisms and Treatments
- Cancer-related molecular mechanisms research
- Genetics and Neurodevelopmental Disorders
- S100 Proteins and Annexins
- NF-κB Signaling Pathways
- Tryptophan and brain disorders
- Wound Healing and Treatments
- RNA Interference and Gene Delivery
- Barrier Structure and Function Studies
- Epigenetics and DNA Methylation
University of Miami
2015-2024
Neurological Surgery
2011-2024
Virginia–Maryland College of Veterinary Medicine
2013
University of Michigan–Ann Arbor
2009
Ludwig-Maximilians-Universität München
2006
The University of Texas Southwestern Medical Center
1997-2001
Southwestern Medical Center
1999
Ohio University
1992-1998
Northeast Ohio Medical University
1992-1998
Baylor College of Medicine
1990-1991
Gene-targeting experiments of Trk receptors and neurotrophins has confirmed the expectation that embryonic sensory sympathetic neurons require neurotrophin function for survival. They have further revealed correlation between a specific requirement eventual modality. We analyzed neonatal mice with mutations in BDNF, 3 (NT-3), TrkC genes. Our data confirm an unexpectedly high proportion neuron losses NT-3 (>70%), BDNF (>20%), (>30%) mutants, which encompass populations thought to be...
It is becoming increasingly clear that brain injuries from a variety of causes stimulate neurogenesis within the hippocampus. remains unclear, however, how robust this response may be and what primary cell types are involved. Here, using controlled cortical impact model traumatic injury on previously characterized transgenic mouse line expresses enhanced green fluorescent protein (eGFP) under control nestin promoter, we demonstrate it earliest type-1 quiescent progenitor cells induced to...
The transcription factor nuclear kappa B (NF-κB) is a key regulator of inflammatory processes in reactive glial cells. We utilized transgenic mouse model (GFAP-IκBα-dn) where the classical NF-κB pathway inactivated by overexpression dominant negative (dn) form inhibitor (IκBα) fibrillary acidic protein (GFAP)-expressing cells, which include astrocytes, Schwann and satellite cells dorsal root ganglion (DRG) sought to determine whether inhibition leads reduction pain behavior inflammation...
After traumatic brain injury (TBI), glial cells have both beneficial and deleterious roles in progression recovery. However, few studies examined the influence of reactive astrocytes tripartite synapse following TBI. Here, we demonstrated that hippocampal synaptic damage caused by controlled cortical impact (CCI) mice results a switch from neuronal to astrocytic d-serine release. Under nonpathological conditions, functions as neurotransmitter coagonist for NMDA receptors is involved...
Abstract Ephrins and Eph receptors are a family of molecules that have been implicated in axonal pathfinding. A unique feature B‐class ephrins is their ability to transmit bidirectional signals both ephrin‐ receptor‐expressing cells upon cell–cell contact. These can lead cytoskeletal alterations attributed regulating neuronal growth responses. Examination gene‐target knockout mice has supported this hypothesis, revealing numerous developmental defects the nervous systems mutant for...
Agenesis of the corpus callosum (CC) is a rare birth defect that occurs in isolated conditions and combination with other developmental cerebral abnormalities. Recent identification families growth guidance molecules has generated interest mechanisms regulate callosal growth. One family, ephrins Eph receptors, been implicated mediating midline pathfinding decisions; however, complexity these interactions yet to be unraveled. Our studies shed light on which B-class receptors function CC how...
Abstract Ephrins and Eph receptor(s) have recently been implicated in regulating neurogenesis the adult subventricular zone (SVZ) rostral migratory stream. Here, we examined role of ephrinB3-EphB3 signaling mediating SVZ response to traumatic brain injury (TBI). Analysis EphB3 expression showed colocalization with glial fibrillary acidic protein-positive neural stem progenitor cells (NSPCs) doublecortin-positive neuroblasts, whereas ephrinB3 was expressed outside neurogenic region. TBI...
There is growing evidence that astrocytes play critical roles in neuron-glial interactions at the synapse. Astrocytes are believed to regulate presynaptic and postsynaptic structures functions, part, by release of gliotransmitters such as glutamate, ATP, d -serine; however, little known how neurons communicate these processes. Here, we investigated a family transmembrane proteins called ephrinBs Eph receptors expressed synapse synaptic transmission plasticity. In addition their presence on...
Compounds targeting the sigma 2 receptor, which we recently cloned and showed to be identical with transmembrane protein 97 (σ2R/TMEM97), are broadly applicable therapeutic agents currently in clinical trials for imaging breast cancer treatment of Alzheimer's disease schizophrenia. These promising applications coupled our previous observation that σ2R/TMEM97 modulator SAS-0132 has neuroprotective attributes improves cognition wild-type mice suggests modulating may also have benefits other...
The sigma 2 receptor (σ
Neurotrophin-3 (NT-3) and its receptor TrkC are known to be important for neuronal survival. More recently, NT-3 has been implicated as playing a role in oligodendrocyte (OL) proliferation survival vitro. Examination of knockout mice revealed reduction NT-3-dependent neurons. To date, no study examined alterations glial cell populations these mice. In this report, we demonstrate decline OL progenitor numbers within the CNS We also observed that immature mature OL-specific markers were...
Abstract Synaptic damage is one of the most prevalent pathophysiological responses to traumatic CNS injury and underlies much associated cognitive dysfunction; however, it poorly understood. The D‐amino acid, D‐serine, serves as primary co‐agonist at synaptic NMDA receptors (NDMARs) a critical mediator NMDAR‐dependent transmission plasticity. In physiological conditions, D‐serine produced released by neurons from enzymatic conversion L‐serine serine racemase (SRR). However, under...
Although a myriad of pathological responses contribute to traumatic brain injury (TBI), cerebral dysfunction has been closely linked cell death mechanisms. A number therapeutic strategies have studied in an attempt minimize or ameliorate tissue damage; however, few studies evaluated the inherent protective capacity brain. Endogenous neural stem/progenitor cells (NSPCs) reside distinct regions and shown respond damage by migrating injury. Until now, it remained unknown whether these promote...
It was previously reported that a tube holding chitosan carriers loaded with neurotrophin-3 (NT-3), after insertion into 5 mm long transection gap in the adult rat spinal cord, triggered de novo neural tissue generation and functional recovery. Here, we report an effort to validate these findings using stringent blinding methodologies, which are crucial for robustness reproducing biomedical studies. Radio frequency identification (RFID) chips were utilized label rats randomly assigned three...
Traumatic brain injury (TBI), ranging from mild concussion to severe penetrating wounds, can involve regions that contain damaged or lost synapses in the absence of neuronal death. These affected significantly contribute sensory, motor and/or cognitive deficits. Thus, studying mechanisms responsible for synaptic instability and dysfunction is important protecting nervous system consequences progressive TBI. Our controlled cortical impact (CCI) produces ~20% loss changes protein levels...
Peripheral ganglion neurons confer sensory information including touch, pain, temperature, and proprioception. Sensory modality is linked to specific neurotrophin (NTF) requirements. NT-3 supports survival of that differentiate primarily into proprioceptors whereas nerve growth factor brain-derived neurotrophic (BDNF) support subpopulations transmit nociception mechanoreception, respectively. We examined gene-targeted mouse mutants at the NT-4, BDNF, NT-3, TrkA loci. show NT-4 functions...
With the discovery of postnatal stem cells within brain, it has become important to understand how extracellular factors might affect maturation neuronal precursors in brain. Neurotrophic are known play a role development but display pleiotrophic effects, part because their physiological interactions with other factors. One factor positioned interact neurotrophins brains animals is atrial C-type natriuretic peptide (CNP). In this study, we used olfactory receptor neurons (ORNs) as model,...