A5004676114- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Acute Lymphoblastic Leukemia research
- Cancer-related Molecular Pathways
- Endoplasmic Reticulum Stress and Disease
- Cancer Mechanisms and Therapy
- Monoclonal and Polyclonal Antibodies Research
- Cell death mechanisms and regulation
- Calcium signaling and nucleotide metabolism
- Cancer, Lipids, and Metabolism
- Cystic Fibrosis Research Advances
- Viral-associated cancers and disorders
- Cell Adhesion Molecules Research
- Legionella and Acanthamoeba research
- Water Treatment and Disinfection
- Bacterial biofilms and quorum sensing
- Extracellular vesicles in disease
- Ubiquitin and proteasome pathways
- Biochemical and Molecular Research
- Galectins and Cancer Biology
- Advanced Breast Cancer Therapies
- PI3K/AKT/mTOR signaling in cancer
- Hematopoietic Stem Cell Transplantation
- Ocular Diseases and Behçet’s Syndrome
Flinders University
2020-2025
Flinders Medical Centre
2020-2025
Royal North Shore Hospital
2013-2024
The University of Sydney
2014-2024
Australasian Leukaemia and Lymphoma Group
2011-2021
Deutsche Bank (Germany)
2017-2021
Northern Sydney Local Health District
2020
Keogh Institute for Medical Research
2017
Royal Bournemouth Hospital
2005-2016
Sydney Adventist Hospital
2013
Extracellular vesicles (EV) are membranous particles (30-1,000 nm in diameter) secreted by cells. Important biological functions have been attributed to 2 subsets of EV, the exosomes (bud from endosomal membranes) and microvesicles (MV; bud plasma membranes). Since both types contain surface proteins derived their cell origin, detection blood may enable diagnosis prognosis disease. We used an antibody microarray (DotScan) compare protein profiles live cancer cells with those based on binding...
Abstract Aims To investigate the changes in polypharmacy and drug burden index ( DBI ) occurring during hospitalisation for older people. The secondary aim was to examine associations of these two measures with length hospital stay admission falls or delirium. Methods A retrospective analysis patients' medical records undertaken at a large university teaching S ydney, ustralia) patients age ≥65 years admitted under care geriatric medicine rehabilitation teams. Polypharmacy defined as use...
Background Many prognostic markers have been identified in chronic lymphocytic leukemia, but there few opportunities to assess their relative importance a large randomized trial. The aim of this study was determine which the available independently predicted outcome patients requiring treatment and use these define new risk groups.Design Methods A broad panel clinical laboratory markers, measured at randomization entering LRF CLL4 trial, assessed with respect response, progression-free...
Hypogammaglobulinemia is a common complication of chronic lymphocytic leukemia (CLL), but the significance immunoglobulin G (IgG) subclass deficiency unknown. We analyzed prevalence immunoglobulins G, A and M, IgG infection in 150 patients with CLL. Low IgG, IgA IgM levels were observed 27.3%, 30.7% 56.7% patients, respectively. was frequent, reduced IgG1, IgG2, IgG3 IgG4 28%, 19.3%, 52% 22.7% (total 64.6%) hypogammaglobulinemia (27.3%) more prevalent than clinically significant (16%)....
Legionella pneumophila is a waterborne pathogen and, as the causative agent of Legionnaires' disease, significant public health concern. Exposure to environmental stresses, and disinfection treatments, promotes formation resistant potentially infectious viable but non-culturable (VBNC) Legionella. The management engineered water systems prevent disease hindered by presence VBNC that cannot be detected using standard culture (ISO11731:2017-05) quantitative polymerase reaction...
Vibrio spp. are opportunistic human and animal pathogens found ubiquitously in marine environments. Globally, there is a predicted rise the prevalence of due to increasing ocean temperatures, which carries significant implications for public health seafood industry. Consequently, an urgent need enhanced strategies control prevent contamination, particularly aquaculture processing facilities. Presently, these industries employ various disinfectants, including benzalkonium chloride (BAC), as...
Abstract Background Cyclin-dependent kinase 9 (CDK9) stimulates oncogenic transcriptional pathways in cancer and CDK9 inhibitors have emerged as promising therapeutic candidates. Methods The activity of an orally bioavailable inhibitor, CDKI-73, was evaluated prostate cell lines, a xenograft mouse model, patient-derived tumor explants organoids. Expression clinical specimens by mining public datasets immunohistochemistry. Effects CDKI-73 on cells were determined cell-based assays, molecular...
Immune dysfunction attributed to hypogammaglobulinaemia is common in chronic lymphocytic leukaemia (CLL) and infection a major contributor morbidity mortality. A higher incidence of multiple immunoglobulin G (IgG) subclass deficiency was associated with more advanced disease (P < 0·001 P 0·001, respectively) cohort 147 CLL patients. Multiple IgG were significantly shorter treatment-free survival (TFS) = 0·006, respectively). The association between stage immune demonstrated by these data...
Background/Objectives: Acute myeloid leukemia (AML) is an aggressive neoplasm. Although most patients respond to induction therapy, they commonly relapse due recurrent disease in the bone marrow microenvironment (BMME). So, disruption of BMME, releasing tumor cells into peripheral circulation, has therapeutic potential. Methods: Using both primary donor AML and cell lines, we developed vitro co-culture model BMME. We used this identify effective agent(s) block adherence reverse...
Monolayer cultures of rat fetal distal lung epithelial (FDLE) cells generated larger spontaneous short circuit currents (ISC) when maintained (48 h) at neonatal alveolar PO2 (100 mmHg) than (23 mmHg). When were shifted between these atmospheres in order to impose a rise equivalent that seen birth, no ISC was after 6 h but the response fully established by 24 h. Studies basolaterally permeabilised revealed small apical Na+ conductance (GNa) raised further change had occurred A substantial...
Small extracellular vesicles (sEV) have emerged as a potential rich source of biomarkers in human blood and present the intriguing for ‘liquid biopsy’ to track disease effectiveness interventions. Recently, we further demonstrated EV derived account variability drug exposure. This study sought evaluate abundance cargo global liver-specific circulating sEV, within (diurnal) between individuals cohort healthy subjects (n = 10). We normal ranges concentration size expression generic protein...
Background: Deletion of phenylalanine-508 (ΔF508) from the first nucleotide-binding domain (NBD1) in wild-type cystic fibrosis (CF) transmembrane-conductance regulator (wtCFTR) causes CF. However, mechanistic relationship between ΔF508-CFTR and diversity CF disease is unexplained. The surface location F508 on NBD1 creates potential for protein-protein interactions nearby, lies a consensus sequence (SYDE) reported to control pleiotropic protein kinase CK2. Methods: Electrophysiology,...
Chronic lymphocytic leukemia (CLL) is clinically heterogeneous. While some patients have indolent disease for many years, 20-30% will progress and ultimately die of their disease. CLL may be classified by the Rai or Binet staging system, mutational status immunoglobulin variable heavy-chain gene (IGVH), ZAP-70 overexpression, cytogenetic abnormalities (13q-, + 12, 11q-, 17p-) expression several cell surface antigens (CD38, CD49d) that correlate with risk progression. However, none these...
Chronic lymphocytic leukaemia (CLL) is invariably accompanied by some degree of immune failure, and CLL patients have a high rate second primary malignancy (SPM) compared to the general population. We comprehensively documented incidence all forms SPM including skin cancer (SC), solid organ (SOM), haematological (SHM) separately Richter's syndrome (RS) across therapy eras. Among 517 CLL/small lymphoma (SLL) patients, overall SPMs with competing risks was SC 31.07%, SOM 25.99%, SHM 5.19% RS...
Interleukin (IL)-6 is an inflammatory cytokine present in the eye during non-infectious uveitis, where it contributes to progression of inflammation. There are two major IL-6 signaling pathways: classic and trans-signaling. Classic requires cellular expression receptor (IL-6R), which exists membrane-bound (mIL-6R) soluble (sIL-6R) forms. Prevailing dogma that vascular endothelial cells do not produce IL-6R, relying on trans-signaling However, literature inconsistent, including with respect...