A5016051937- Milk Quality and Mastitis in Dairy Cows
- Probiotics and Fermented Foods
- Identification and Quantification in Food
- Microbial infections and disease research
- Molecular Biology Techniques and Applications
- Infant Nutrition and Health
- Antimicrobial Resistance in Staphylococcus
- Mycobacterium research and diagnosis
- Antimicrobial Peptides and Activities
- QR Code Applications and Technologies
- Gut microbiota and health
- Computational Drug Discovery Methods
- Natural Antidiabetic Agents Studies
- Bacteriophages and microbial interactions
- Neonatal and Maternal Infections
- Genetic and phenotypic traits in livestock
- Diet, Metabolism, and Disease
- Antibiotic Resistance in Bacteria
National Institute Of Veterinary Epidemiology And Disease Informatics
2011-2019
Kerala Veterinary and Animal Sciences University
2016
Maharashtra Animal and Fishery Sciences University
2016
To develop and evaluate a multiplex PCR (mPCR) assay for simultaneous detection of 10 bacterial species causing bovine mastitis namely, Staphylococcus aureus, chromogenes, epidermidis, sciuri, haemolyticus, simulans, Streptococcus agalactiae, dysgalactiae, uberis Escherichia coli in milk.A two-tube mPCR was developed. The accuracy the evaluated using 56 standard reference strains 705 comprising E. (n = 99), staphylococci 522) streptococci 84). threshold fg genomic DNA <10(3) CFU ml(-1). A...
Aim To evaluate the virulence determinants and genetic diversity of Staphylococcus aureus from bovine subclinical mastitis milk. Methods Results PCR detection genes was performed for 173 Staph. Further, analysed by agr spa typing followed pulsed field gel electrophoresis (PFGE) selected isolates. Screening (n = 19) showed adherence viz. fnbA, clfA, fnbB cna in 98·8, 97·1, 68·8 28·3 percentage isolates, respectively, 80 strains (46·24%) positive enterotoxin were distributed as 23 toxinotypes,...
Mastitis is a multietiological complex disease, defined as inflammation of parenchyma mammary glands. Bacterial infection the predominant cause mastitis, though fungal, viral and mycoplasma infections also have been reported. Based on severity mastitis can be classified into subclinical, clinical chronic forms. pathogens from fresh cow milk were isolated by standard microbiological tests multiplex PCR. Epidemiological studies shown that Escherichia coli second largest pathogen after...
There is renewed interest towards understanding the host-pathogen interaction in light of epigenetic modifications. Although epithelial tissue major site for interactions, there handful studies to show how cells respond pathogens. Bacterial infection mammary gland parenchyma induces local and subsequently systemic inflammation that results a complex disease called mastitis. Globally Staphylococcus aureus single largest mastitis pathogen can ultimately result either subclinical or chronic...
Escherichia coli is a normal inhabitant of gastrointestinal tract humans and animals one the most important causes bovine mastitis. Definitive identification E. from other members Enterobacteriaceae remains ambiguous. The present study describes duplex PCR, targeting 2 housekeeping genes, lacy (lactose permease) phoA (alkaline phosphatase) for reliable detection that enables its differentiation biochemically phylogenetically related bacteria. assay was evaluated employing 19 ATCC (American...
Objective: Species identification of Staphylococcus is the prerequisite for precise assessment of
 microbial dynamics including their transmission and pathogenic significance in
 dairy herd environment. The present study aimed to develop simplex PCR
 assays rapid specific nine different species. 
 Methods: Specific
 primers targeting sodA gene S. aureus, chromogenes, hominis,
 haemolyticus, hyicus; gap sciuri, auricularis, simulans;
 rdr epidermidis were...
Diabetes mellitus remains to be a significant global metabolic disorder, affecting both human and animal populations. Plant-derived medications have gained focus for diabetes management due their perceived safety compared conventional drugs. Recent research has emphasized the potential of polyphenols, particularly flavonols, in modulating genes related insulin secretion signalings pathways, indicating role preventing type II diabetes. This study investigates interactions three plant...
To improve mastitis diagnosis and achieve rapid, specific, reliable cost effective test, a multiplex PCR for simultaneous detection differentiation of major streptococcal species, viz. Streptococcus agalactiae, uberis dysgalactiae was developed. Evaluation with 24 ATCC strains 606 comprising streptococci (84) staphylococci (522) showed the assay to be highly accurate. The threshold mPCR 10fg genomic DNA < 102 CFU ml-1. Assessment 115 milk samples collected from subclinically infected...