A5075181025- Glaucoma and retinal disorders
- Ubiquitin and proteasome pathways
- IoT and Edge/Fog Computing
- Ocular Surface and Contact Lens
- Computational Drug Discovery Methods
- Phytoplasmas and Hemiptera pathogens
- Protein Structure and Dynamics
- Histone Deacetylase Inhibitors Research
- Malaria Research and Control
- Epigenetics and DNA Methylation
- Milk Quality and Mastitis in Dairy Cows
- Autophagy in Disease and Therapy
- Insect-Plant Interactions and Control
- Silkworms and Sericulture Research
- Insect symbiosis and bacterial influences
- Context-Aware Activity Recognition Systems
- HIV/AIDS drug development and treatment
- Plant Pathogenic Bacteria Studies
- Sirtuins and Resveratrol in Medicine
- Retinal Diseases and Treatments
- Metabolomics and Mass Spectrometry Studies
- Enzyme Structure and Function
- Neuroblastoma Research and Treatments
- Biochemical Acid Research Studies
- Antimicrobial agents and applications
KIIT University
2017-2024
Indian Institute of Technology Bhubaneswar
2020-2024
Maulana Abul Kalam Azad University of Technology, West Bengal
2023-2024
Jawaharlal Nehru Centre for Advanced Scientific Research
2003-2014
The emergence of drug-resistant forms Plasmodium falciparum emphasizes the need to develop new antimalarials. In this context, fatty acid biosynthesis (FAS) pathway malarial parasite has recently received a lot attention. Due differences in systems and man, is good target for development selective therapeutic drugs directed against malaria. continuation these efforts we report cloning overexpression P. β-hydroxyacyl-acyl carrier protein (ACP) dehydratase (PffabZ) gene that codes 17-kDa...
PCAF (KAT2B) belongs to the GNAT family of lysine acetyltransferases (KAT) and specifically acetylates histone H3K9 residue several nonhistone proteins. is also a transcriptional coactivator. Due lack KAT-specific small molecule inhibitor, exclusive role acetyltransferase activity not well understood. Here, we report that natural compound hydroxybenzoquinone class, embelin, inhibits H3Lys9 acetylation in mice recombinant PCAF-mediated with near complete specificity vitro. Furthermore, using...
Purpose: To evaluate the differential expression of tear matrix metalloproteinases (MMP) 2 and 9 in patients with various forms glaucoma. Methods: Tear samples were collected a Schirmer's strip from 148 eyes 113 (medically naïve primary open-angle [POAG] or angle closure glaucoma [PACG] those pseudoexfoliation syndrome [PXF] [PXG]). These compared to undergoing cataract surgery (controls) for this cross-sectional study. Functional activities MMP-9 MMP-2 analyzed by gelatin zymography....
Mastitis is a multietiological complex disease, defined as inflammation of parenchyma mammary glands. Bacterial infection the predominant cause mastitis, though fungal, viral and mycoplasma infections also have been reported. Based on severity mastitis can be classified into subclinical, clinical chronic forms. pathogens from fresh cow milk were isolated by standard microbiological tests multiplex PCR. Epidemiological studies shown that Escherichia coli second largest pathogen after...
Self-assembled highly luminescent nanoscale coordination polymer of {[Gd(OPE)(NO3)(H2O)2]·H2O} (NCP-1), (oligo-(p-phenyleneethynylene)dicarboxylate) was synthesized by coordination-driven self-assembly oligo-(p-phenyleneethynylene)dicarboxylic acid and GdIII in polar solvent under refluxing conditions. This nanostructure has been characterized FESEM, TEM, powder X-ray diffraction, adsorption study. Interdigitation between 1D polymers through alkyl chains results a porous supramolecular 3D...
There is renewed interest towards understanding the host-pathogen interaction in light of epigenetic modifications. Although epithelial tissue major site for interactions, there handful studies to show how cells respond pathogens. Bacterial infection mammary gland parenchyma induces local and subsequently systemic inflammation that results a complex disease called mastitis. Globally Staphylococcus aureus single largest mastitis pathogen can ultimately result either subclinical or chronic...
Purpose Pseudoexfoliation (PXF) is a unique form of glaucoma characterized by accumulation exfoliative material in the eyes. Changes tear profile disease stages may give us insights into molecular mechanisms involved causing eye. Methods All patients were categorized three main categories; pseudoexfoliation (PXF), (PXG) and cataract, which served as control. Cytokines, transforming growth factor β1 (TGFβ1), matrix metalloproteases (MMPs) fibronectin (FN1) assessed with multiplex bead assay,...
Prokaryotic deacetylases are classified into nicotinamide adenine dinucleotide (NAD+)-dependent sirtuins and Zn2+-dependent deacetylases. NAD+ is a coenzyme for redox reactions, thus serving as an essential component energy metabolism. The NAD+-dependent deacetylase domain quite conserved well characterized across bacterial species like CobB in Escherichia coli Salmonella, Rv1151c Mycobacterium, SirtN Bacillus subtilis. E. the only with known crystal structure (PDB ID: 1S5P), which has 91%...
The emergence of strains Plasmodium falciparum resistant to the commonly used antimalarials warrants development new antimalarial agents. discovery type II fatty acid synthase (FAS) in distinct from FAS its human host (type I FAS) opened up avenues for novel antimalarials. process synthesis takes place by iterative elongation butyryl-acyl carrier protein (butyryl-ACP) two carbon units, with successive action four enzymes constituting module until desired acyl length is obtained. study...
Purpose Prostaglandin analogues (PGA’s) are the mainstay and first line of treatment in current glaucoma practise. Though latanoprost bimatoprost most commonly used PGA’s with minimal side effects at lower concentrations like bimaotoprost 0.01%, direct comparison their cytokine/MMP profile tears has not been evaluated earlier. The study intends to ascribe PGA upregulation MMPs, Cytokines Chemokines mediating varied pathways result drugs. Methods Tear sample collection was done from outer...
Retinoblastoma is usually initiated by biallelic RB1 gene inactivation. In addition, MYCN copy number alterations also contribute to RB pathogenesis. However, expression, its role in disease progression and correlation with histological risk factors are not well understood. We studied the expression of enucleated patient specimens immunohistochemistry. overexpressed compared control retina. Our microarray analysis followed qRT-PCR validation revealed that genes involved glucose metabolism...
The key differences in cell death mechanisms the trabecular meshwork (TM) adult moderate and severe primary glaucoma remain still unanswered. This study explored glaucoma.In-vitro laboratory on surgical specimens lines.Select death-related proteins differentially expressed mass spectrometric analysis ex-vivo dissected TM patients with open-angle (POAG) or angle-closure (PACG) compared to controls (cadaver donor cornea) were validated for temporal changes gene expression in-vitro human...
The unfolding pathways of the two forms Plasmodium falciparum acyl carrier protein, apo and holo forms, were determined by guanidine hydrochloride‐induced denaturation. Both form displayed a reversible two‐state mechanism. analysis isothermal denaturation data provides values for conformational stability proteins. Although both have same amino acid sequence, they similar secondary structures, it was found that – Δ G lower than at all temperatures which experiments done. higher can be...
The type II fatty acid synthase (FAS) pathway of Plasmodium falciparum is a validated unique target for developing novel antimalarials, due to its intrinsic differences from the typeI operating in humans. beta-Ketoacyl acyl carrier protein (ACP) reductase (FabG) performs NADPH-dependent reduction beta-ketoacyl-ACP beta-hydroxyacyl-ACP, first reductive step elongation cycle biosynthesis. In this article, we report intensive studies on direct interactions FabG and ACP solution, presence...