Two component injectable hydrogels that cross-link in situ have been used as noninvasive wound-filling devices, i.e., sealants. These materials carry a variety of functions at the wound sites, such sealing leaks, ceasing unwanted bleeding, binding tissues together, and assisting healing processes. However, commonly sealants typically lack antibacterial properties. Since bacterial infection site is very common, bioadhesive with intrinsic properties are urgently required. Herein, we report...

Abstract A contercurrent distribution system has been devised for bovine thyrotropin which permits easy removal of several impurities, including one the peptide chains luteinizing hormone. The remaining chain hormone, a number properties similar to those thyrotropin, can then be removed by gel filtration. is biologically active after countercurrent and no evidence found separation subunits as occurs with contains more methionine, tyrosine, lysine than previously reported. Electrophoresis...

The continuous rise of antimicrobial resistance and the dearth new antibiotics in clinical pipeline raise an urgent call for development potent agents. Cationic chitosan derivatives, N-(2-hydroxypropyl)-3-trimethylammonium chlorides (HTCC), have been widely studied as antibacterial However, their systemic structure–activity relationship, activity toward drug-resistant bacteria fungi, mode action are very rare. Moreover, toxicity efficacy these polymers under vivo conditions yet to be...

To develop and evaluate a multiplex PCR (mPCR) assay for simultaneous detection of 10 bacterial species causing bovine mastitis namely, Staphylococcus aureus, chromogenes, epidermidis, sciuri, haemolyticus, simulans, Streptococcus agalactiae, dysgalactiae, uberis Escherichia coli in milk.A two-tube mPCR was developed. The accuracy the evaluated using 56 standard reference strains 705 comprising E. (n = 99), staphylococci 522) streptococci 84). threshold fg genomic DNA <10(3) CFU ml(-1). A...

The emergence of drug resistance along with a declining pipeline clinically useful antibiotics has made it vital to develop more effective antimicrobial therapeutics, particularly against difficult-to-treat Gram-negative pathogens (GNPs). Many antibacterial agents, including glycopeptide such as vancomycin, are inherently inactive toward GNPs because their inability cross the outer membrane these pathogens. Here, we demonstrate, for first time, lipophilic cationic (permanent positive charge)...

Cationic-amphiphilic antibacterial polymers with optimal amphiphilicity generally target the bacterial membranes instead of mammalian membranes. To date, this balance has been achieved by varying cationic charge or side chain hydrophobicity in a variety cationic-amphiphilic polymers. Optimal considered as governing factor for potent activity yet minimal cell toxicity. However, concomitant role hydrogen bonding and constant interactions is not understood. Also, degradable that result nontoxic...

Abstract Resistance to glycopeptide antibiotics, the drugs of choice for life‐threatening bacterial infections, is on rise. In order counter threat glycopeptide‐resistant bacteria, we report development a new class semi‐synthetic which not only target membrane but also display enhanced inhibition cell‐wall biosynthesis through increased binding affinity their peptides. The combined effect these two mechanisms resulted in improved vitro activity three orders magnitude over vancomycin and no...

is a recently described species that can be differentiated from

Background Epidemiological risk factors such as the demography of a place, environment, food, livestock, and companion animals are known sources Klebsiella pneumoniae infection. Whole-genome sequencing (WGS) has become powerful tool to complement traditional microbiological characterization foodborne pathogens. Moreover, K. several species complexes (KpSC) is very difficult differentiate using routine methods. The present study aims investigate prevalence in fish available retail market WGS....

Porcine epidemic diarrhea (PED) is a significant pig disease causing high mortality in suckling pigs and morbidity across all age groups. It highly prevalent Southeast Asia, posing threat of transboundary transmission to India. Although antibodies were detected as early 2003 Assam, there was no evidence viral detection or molecular characterization until this study. This study reports the first clinical outbreak PED India, followed by genetic virus (PEDV) during 2022-23. The characterized,...

Development of synthetic strategies to combat Staphylococcal infections, especially those caused by methicillin resistant Staphyloccus aureus (MRSA), needs immediate attention. In this manuscript we report the ability aryl-alkyl-lysines, simple membrane active small molecules, treat infections planktonic cells, persister cells and biofilms MRSA. A representative compound, NCK-10, did not induce development resistance in multiple passages retained activity varying environments pH salinity. At...

The study describes prevalence, clinical symptoms and risk factors for brucellosis in personnel engaged veterinary health care Karnataka, India. A total of 1050 sera samples were collected from animal handlers, veterinarians, students, para-veterinarians persons artificial insemination animals. tested by Rose Bengal plate test (RBPT), serum agglutination (SAT), IgG IgM indirect ELISA PCR. Age, sex, recorded structured questionnaire. Of the tested, 6.76, 6.38, 3.90, 2.67 2.0% positive ELISA,...

New Delhi metallo-β-lactamase-1 (NDM-1) is the major contributor to emergence of carbapenem resistance in Gram-negative pathogens (GNPs) and has caused many clinically available β-lactam antibiotics become obsolete. A approved inhibitor metallo-β-lactamase (MBL) that could restore activity carbapenems against resistant GNPs not yet been found, making NDM-1 a serious threat human health. Here, we have rationally developed an for enzyme, which ability penetrate outer membrane inactivate enzyme...

This study was aimed to understand the temporal and spatial epidemiology of peste des petits ruminants (PPR) in India using national surveillance data available National Animal Diseases Referral Expert System (NADRES) along with its control plan undertaken. On analysis outbreaks/cases reports sheep goats NADRES database from 1995 2019, it observed that PPR features among top ten diseases stands first viral diseases, reported deaths, accounts for 36% mortality goats. outbreaks occur round...

Aim To evaluate the virulence determinants and genetic diversity of Staphylococcus aureus from bovine subclinical mastitis milk. Methods Results PCR detection genes was performed for 173 Staph. Further, analysed by agr spa typing followed pulsed field gel electrophoresis (PFGE) selected isolates. Screening (n = 19) showed adherence viz. fnbA, clfA, fnbB cna in 98·8, 97·1, 68·8 28·3 percentage isolates, respectively, 80 strains (46·24%) positive enterotoxin were distributed as 23 toxinotypes,...

Mastitis is a multietiological complex disease, defined as inflammation of parenchyma mammary glands. Bacterial infection the predominant cause mastitis, though fungal, viral and mycoplasma infections also have been reported. Based on severity mastitis can be classified into subclinical, clinical chronic forms. pathogens from fresh cow milk were isolated by standard microbiological tests multiplex PCR. Epidemiological studies shown that Escherichia coli second largest pathogen after...

Gram-negative 'superbugs' such as New Delhi metallo-beta-lactamase-1 (blaNDM-1) producing pathogens have become world's major public health threats. Development of molecular strategies that can rehabilitate the 'old antibiotics' and halt antibiotic resistance is a promising approach to target them. We report membrane-active macromolecules (MAMs) restore antibacterial efficacy (enhancement by >80-1250 fold) tetracycline antibiotics towards blaNDM-1 Klebsiella pneumonia Escherichia coli...

Chronic bacterial biofilms place a massive burden on healthcare due to the presence of antibiotic-tolerant dormant bacteria. Some conventional antibiotics such as erythromycin, vancomycin, linezolid, rifampicin etc. are inherently ineffective against Gram-negative bacteria, particularly in their biofilms. Here, we report membrane-active macromolecules that kill slow dividing stationary-phase and antibiotic tolerant cells More importantly, these molecules potentiate (erythromycin rifampicin)...