A5067510341- Antimicrobial Peptides and Activities
- Antimicrobial agents and applications
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Antifungal resistance and susceptibility
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Antimicrobial Resistance in Staphylococcus
- Lipid Membrane Structure and Behavior
- Viral Infections and Vectors
- Biochemical and Structural Characterization
- Click Chemistry and Applications
- Probiotics and Fermented Foods
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Antibiotic Use and Resistance
- Viral Infections and Outbreaks Research
- Polydiacetylene-based materials and applications
- Plant-Microbe Interactions and Immunity
- Clostridium difficile and Clostridium perfringens research
- Immune Response and Inflammation
- Fungal Infections and Studies
- Immunotherapy and Immune Responses
- Bacterial Genetics and Biotechnology
Helmholtz Institute for RNA-based Infection Research
2023-2025
Helmholtz Centre for Infection Research
2023
Jawaharlal Nehru Centre for Advanced Scientific Research
2014-2022
Max Planck Institute of Colloids and Interfaces
2020-2022
Sukkur IBA University
2017
The emergence of multidrug resistant bacteria compounded by the depleting arsenal antibiotics has accelerated efforts toward development with novel mechanisms action. In this report, we present a series small molecular antibacterial peptoid mimics which exhibit high in vitro potency against variety Gram-positive and Gram-negative bacteria, including drug-resistant species such as methicillin-resistant Staphylococcus aureus vancomycin-resistant Enterococcus faecium. highlight these compounds...
Natural and synthetic membrane active antibacterial agents offer hope as potential solutions to the problem of bacterial resistance membrane-active nature imparts low propensity for development resistance. In this report norspermidine based molecules were developed that displayed excellent activity against various wild-type bacteria (Gram-positive Gram-negative) drug-resistant (methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus faecium, β-lactam-resistant...
Membrane-active amphiphilic small molecules selectively kill bacteria over mammalian cells, disperse preformed biofilms and reduce bacterial resistance development.
Bacterial colonization and subsequent formation of biofilms onto surfaces medical devices implants is a major source nosocomial infections. Most antibacterial coatings to combat infections are either metal-based or nondegradable-polymer-based hence limited by their nondegradability unpredictable toxicity. Moreover, effectively, the required display simultaneous antibiofilm activity. Herein we report biocompatible biodegradable based on organo-soluble quaternary chitin polymers which were...
Malaria, a mosquito-borne disease caused by Plasmodium species, claims more than 400,000 lives globally each year. The increasing drug resistance of the parasite renders development new anti-malaria drugs necessary. Alternatively, better delivery systems for already marketed could help to solve problem. Herein, we report glucose-based ultra-small gold nanoparticles (Glc-NCs) that bind cysteine-rich domains falciparum surface proteins. Microscopy shows Glc-NCs specifically extracellular and...
In light of the recent outbreak Ebola virus (EBOV) disease in West Africa, there have been renewed efforts to search for effective antiviral countermeasures. A range compounds currently available with broad antimicrobial activity tested against EBOV. Using live EBOV, eighteen candidate were screened vitro. The selected on a rational basis because their mechanisms action suggested that they had potential disrupt EBOV entry, replication or exit from cells displayed some previous tests. Nine...
Development of synthetic strategies to combat Staphylococcal infections, especially those caused by methicillin resistant Staphyloccus aureus (MRSA), needs immediate attention. In this manuscript we report the ability aryl-alkyl-lysines, simple membrane active small molecules, treat infections planktonic cells, persister cells and biofilms MRSA. A representative compound, NCK-10, did not induce development resistance in multiple passages retained activity varying environments pH salinity. At...
The international symposium ASOBIOTICS 2024 brought together scientists across disciplines to discuss the challenges of advancing antibacterial antisense oligomers (ASOs) from basic research clinical application. Hosted by Helmholtz Institute for RNA-based Infection Research (HIRI) in Wurzburg, Germany, on September 12-13th, 2024, event featured presentations covering major milestones and current this antimicrobial technology its applications against pathogens, commensals, bacterial viruses....
Fusobacteria are commensal members of the oral microbiome that can spread from their primary niche and colonize distal sites in human body. Their enrichment colorectal breast cancer tissue has been associated with tumor growth, metastasis, chemotherapeutic resistance. The use non-selective antibiotics to remove fusobacteria impairs progression, but prolonged application risks side effects such as gastrointestinal problems dysbiosis. Species-specific antisense based on peptide nucleic acid...
The emergence of bacterial resistance and biofilm associated infections has created a challenging situation in global health. In this present state affairs where conventional antibiotics are falling short being able to provide solution these problems, development novel antibacterial compounds possessing the twin prowess antibiofilm efficacy is imperative. Herein, we report library amino acid tunable lipidated norspermidine conjugates that were prepared by conjugating both acids fatty with...
Infections caused by drug-resistant Gram-negative pathogens continue to be significant contributors human morbidity. The recent advent of New Delhi metallo-β-lactamase-1 (blaNDM-1) producing pathogens, against which few drugs remain active, has aggravated the problem even further. This paper shows that aryl-alkyl-lysines, membrane-active small molecules, are effective in treating infections pathogens. One compounds study was killing planktonic cells as well dispersing biofilms compound...
Vancomycin is a standard drug for the treatment of multidrug-resistant Gram-positive bacterial infections. Albeit, development resistance (VRE, VRSA) and its inefficacy against persistent infections demerit. It also intrinsically inactive Gram-negative bacteria. Herein, we report vancomycin derivative, VanQAmC10, that addresses these challenges. VanQAmC10 was rapidly bactericidal carbapenem-resistant A. baumannii (6 log10 CFU/mL reduction in 6 h), disrupted biofilms, eradicated their...
Lipidated-biphenyl-lysines that selectively inhibit intra and extracellular <italic>S. aureus</italic> are reported. Active in murine models, they also possess antibiofilm anti-inflammatory properties.
Mortality due to pathogenic fungi has been exacerbated by the rapid development of resistance frontline antifungal drugs. Fungicidal compounds with novel mechanisms action are urgently needed. Aryl-alkyl-lysines, which membrane-active small molecules, were earlier shown be broad-spectrum antibacterial agents potency in vitro and vivo. Herein, we report properties aryl-alkyl-lysines. After identifying most active compound (NCK-10), tested its activity against a panel clinically relevant...
Correction for 'Selective and broad spectrum amphiphilic small molecules to combat bacterial resistance eradicate biofilms' by Jiaul Hoque et al., Chem. Commun., 2015, 51, 13670-13673.
In the global effort to thwart antimicrobial resistance, lipopeptides are an important class of agents, especially against Gram-negative infections. attempt circumvent their synthetic complexities, we designed simple membrane-active agents involving only one amino acid and two lipid tails. Herein show that use short tails instead a single long significantly increases selective antibacterial activity. This study yielded several compounds, investigations into properties this compound were...
Antisense oligomer (ASO)-based antibiotics that target mRNAs of essential bacterial genes have great potential for counteracting antimicrobial resistance and precision microbiome editing. To date, the development such antisense has primarily focused on using phosphorodiamidate morpholino (PMO) peptide nucleic acid (PNA) backbones, largely ignoring growing number chemical modalities spurred success ASO-based human therapy. Here, we directly compare activities seven chemically distinct 10mer...
The emergence of bacterial resistance and hesitance in approving new drugs has bolstered research on membrane-active agents such as antimicrobial peptides their synthetic derivatives therapeutic alternatives against infections. Herein, we document the action aryl-alkyl-lysines liposomes mimicking membranes using solid-state nuclear magnetic resonance spectroscopy. A significant perturbation lipid thickness order parameter membrane was observed upon treatment with this class compounds....