A5071335468- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Peptidase Inhibition and Analysis
- Cancer Research and Treatments
- Polyamine Metabolism and Applications
- Lung Cancer Treatments and Mutations
- Studies on Chitinases and Chitosanases
- Signaling Pathways in Disease
- Aquaculture disease management and microbiota
- CRISPR and Genetic Engineering
- Trace Elements in Health
- Statistical Methods in Clinical Trials
- RNA Interference and Gene Delivery
- Antifungal resistance and susceptibility
- Cytokine Signaling Pathways and Interactions
- Genetic factors in colorectal cancer
- Protease and Inhibitor Mechanisms
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Hepatitis B Virus Studies
- PI3K/AKT/mTOR signaling in cancer
- Molecular Biology Techniques and Applications
- Parasitic Infections and Diagnostics
National Institutes of Health
2011-2025
National Institute of Allergy and Infectious Diseases
2015-2025
University of Utah
2010-2011
Huntsman Cancer Institute
2011
University of Wisconsin–Madison
2008-2009
A cancer-associated signaling pathway is reversibly activated in the normal airways of smokers before they develop lung cancer, presenting an opportunity for preventive therapy.
Salmonella enterica serovar Typhimurium is a common cause of food-borne gastrointestinal illness, but additionally it causes potentially fatal bacteremia in some immunocompromised patients. In mice, systemic spread and replication the bacteria depend upon infection within macrophages, human macrophages not widely reported or well studied. order to assess ability replicate we infected primary monocyte-derived (MDM) that had been differentiated under conditions known generate different...
Type III secretion system 1 (T3SS1) is used by the enteropathogen Salmonella enterica serovar Typhimurium to establish infection in gut. Effector proteins translocated this across plasma membrane facilitate invasion of intestinal epithelial cells. One such effector, inositol phosphatase SopB, contributes and mediates activation pro-survival kinase Akt. Following internalization, some bacteria escape from Salmonella-containing vacuole into cytosol there evidence suggesting that T3SS1...
ABSTRACT In the enteric pathogen Salmonella Typhimurium, invasion and motility are coordinated by HilD, a master regulator that activates expression of genes encoding type III secretion system 1 some genes, including chemotaxis gene mcpC . Previously, we have shown McpC induces smooth swimming, which is important for 1-dependent epithelial cells. Here, studied another -specific gene, mcpA , demonstrate it also HilD regulated. Whereas induction occurs direct derepression H-NS, requires...
Salmonella enterica uses effector proteins translocated by a Type III Secretion System to invade epithelial cells. One of the invasion-associated effectors, SopB, is an inositol phosphatase that mediates sustained activation pro-survival kinase Akt in infected Canonical involves membrane translocation and phosphorylation dependent on phosphatidyl inositide 3 (PI3K). Here we have investigated these two distinct processes HeLa Firstly, found SopB-dependent are insensitive PI3K inhibitor...
Abstract In the enteric pathogen Salmonella enterica serovar Typhimurium, invasion and motility are coordinated by master regulator HilD, which induces expression of type III secretion system 1 (T3SS1) genes. Methyl-accepting chemotaxis proteins (MCPs) detect specific ligands control direction flagellar motor, promoting tumbling changes in (if a repellent is detected) or smooth swimming (in presence an attractant). Here, we show that HilD upregulating uncharacterized MCP (McpC), this...
The intracellular fungal pathogen Histoplasma capsulatum (Hc) resides in mammalian macrophages and causes respiratory systemic disease. Iron limitation is an important host antimicrobial defence, iron acquisition critical for microbial pathogenesis. Hc displays several mechanisms, including secreted glutathione-dependent ferric reductase activity (GSH-FeR). We purified this enzyme from culture supernatant identified a novel extracellular reduction strategy involving gamma-glutamyltransferase...
Here we describe the use of synthetic genetic elements to improve predictability and tunability episomal protein production in Salmonella. We used a multi-pronged approach, which series variable-strength promoters were combined with transcriptional terminator, plasmid copy number variation. This yielded plasmids that drive uniform fluorescent endogenous proteins, over wide dynamic range. several examples where this system is fine-tune constitutive expression Salmonella, providing an...
ABSTRACT Dipeptidyl peptidase type IV (DppIV) enzymes are broadly distributed phylogenetically and display diverse functions, including intercellular signaling, immunomodulation, protein maturation processing, metabolism, nutrient acquisition. We identified a secreted proteolytic activity in Histoplasma capsulatum effective toward DppIV-specific substrates. In order to determine the gene(s) that encodes this activity, we two putative DPPIV homologs ( HcDPPIVA HcDPPIVB ) H. based on homology...
The successful infection of macrophages by non-typhoidal serovars Salmonella enterica is likely essential to the establishment systemic disease they sometimes cause in susceptible human populations. However, interactions between and are not widely studied, with mouse being a much more common model system. Fundamental differences make this less than ideal. Additionally, inability macrophage-like cell lines replicate some properties primary makes use cells desirable. Here we present protocols...
Although collections of formalin fixed paraffin embedded (FFPE) samples exist, sometimes representing decades stored samples, they have not typically been utilized to their full potential. Normal tissue from such would be extremely valuable for generation genotype data individuals who cannot otherwise provide a DNA sample.We extracted normal identified in FFPE blocks prostate surgery and obtained complete genome wide over 500,000 SNP markers these whole blood 2 the cases, comparison.Four...
The pathogenic fungus Histoplasma capsulatum secretes dipeptidyl peptidase (Dpp) IV enzyme activity and has two putative DPPIV homologs (HcDPPIVA HcDPPIVB). We previously showed that HcDPPIVB is the gene responsible for majority of secreted DppIV in H. culture supernatant, while we could not detect any functional contribution from HcDPPIVA. In order to determine whether HcDPPIVA encodes a enzyme, expressed Pichia pastoris purified recombinant protein. cleaved synthetic substrates had similar...
Persistence and intermittent fecal shedding, hallmarks of Salmonella infections, are critical for fecal-oral transmission. In the intestine, enterica serovar Typhimurium (STm) actively invade intestinal epithelial cells survive in Salmonella-containing vacuole (SCV) cell cytosol. Cytosolic STm replicate rapidly, express invasion factors, induce extrusion infected into lumen. Here, we engineered that self-destruct cytosol, but normally SCV, to examine role cytosolic persistence shedding....