A5000507404- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Glycosylation and Glycoproteins Research
- Ferroptosis and cancer prognosis
- Education, Achievement, and Giftedness
- Rheumatoid Arthritis Research and Therapies
- Virus-based gene therapy research
- Career Development and Diversity
- PI3K/AKT/mTOR signaling in cancer
- Supramolecular Self-Assembly in Materials
- Synthesis and Catalytic Reactions
- RNA modifications and cancer
- T-cell and B-cell Immunology
- Neuroblastoma Research and Treatments
- Cancer-related Molecular Pathways
- Systemic Lupus Erythematosus Research
- Motivation and Self-Concept in Sports
- Single-cell and spatial transcriptomics
- Grit, Self-Efficacy, and Motivation
Purdue University West Lafayette
2020-2024
University of Houston
2021-2023
Beijing Obstetrics and Gynecology Hospital
2021-2023
Capital Medical University
2021-2023
Zhengzhou University
2017-2022
Henan Provincial People's Hospital
2022
North Carolina Central University
2021
East China University of Science and Technology
2021
The University of Texas MD Anderson Cancer Center
2011-2020
Juntendo University
2012
T cell-mediated immunotherapies are promising cancer treatments. However, most patients still fail to respond these therapies. The molecular determinants of immune resistance poorly understood. We show that loss PTEN in tumor cells preclinical models melanoma inhibits killing and decreases T-cell trafficking into tumors. In patients, correlates with decreased infiltration at sites, reduced likelihood successful expansion from resected tumors, inferior outcomes PD-1 inhibitor therapy....
Adoptive cell transfer (ACT) is considered a promising modality for cancer treatment, but despite ongoing improvements, many patients do not experience clinical benefits. The tumor microenvironment an important limiting factor in immunotherapy that has been addressed fully ACT treatments. In this study, we report upregualtion of the immunosuppressive receptor programmed death-1 (PD-1) expressed on transferred T cells at site, murine model ACT, compared with its expression present peripheral...
Abstract Purpose: Treatment of melanoma patients with selective BRAF inhibitors results in objective clinical responses the majority BRAF-mutant tumors. However, resistance to these develops within a few months. In this study, we test hypothesis that inhibition combination adoptive T-cell transfer (ACT) will be more effective at inducing long-term regressions Experimental Design: BRAF-mutated human tumor cell lines transduced express gp100 and H-2Db allow recognition by gp100-specific pmel-1...
Abstract Immunotherapy has only limited efficacy against pancreatic ductal adenocarcinoma (PDAC) due to the presence of an immunosuppressive tumor-associated stroma. Here, we demonstrate effective modulation that stroma by irreversible electroporation (IRE), a local ablation technique received regulatory approval in United States. IRE induces immunogenic cell death, activates dendritic cells, and alleviates stroma-induced immunosuppression without depleting tumor-restraining collagen. The...
T-cell-based immunotherapies are promising treatments for cancer patients. Although durable responses can be achieved in some patients, many patients fail to respond these therapies, underscoring the need improvement with combination therapies. From a screen of 850 bioactive compounds, we identify HSP90 inhibitors as candidates immunotherapy. We show that inhibition ganetespib enhances T-cell-mediated killing patient-derived human melanoma cells by their autologous T vitro and potentiates...
Proinflammatory cytokines have been approved by the Food and Drug Administration for treatment of metastatic melanoma renal carcinoma. However, effective cytokine therapy requires high-dose infusions that can result in antidrug antibodies and/or systemic side effects limit long-term benefits. To overcome these limitations, we developed a clinically translatable delivery platform composed polymer-encapsulated human ARPE-19 (RPE) cells produce natural cytokines. Tumor-adjacent administration...
A series of triblock protein copolymers composed a central water-soluble polyelectrolyte segment flanked by two coiled-coil domains was synthesized genetic engineering methods. The self-assembled into reversible hydrogels in response to changes temperature, pH, and the presence or absence denaturating agent (guanidine hydrochloride, GdnHCl). Hydrogel formation concentration-dependent, concentration needed for hydrogel correlated with oligomerization state copolymers. morphology hydrogels, as...
Immunotherapy has increasingly become a staple in cancer treatment. However, substantial limitations the durability of response highlight need for more rational therapeutic combinations. The aim this study is to investigate how make tumor cells sensitive T-cell-based immunotherapy.Two pairs melanoma patient-derived cell lines and their autologous tumor-infiltrating lymphocytes were utilized high-throughput screen 850 compounds identify bioactive agents that could be used combinatorial...
Unmethylated CpG oligodeoxynucleotides (CpG) are synthetic toll-like receptor 9 agonists that activate innate immune cells and which have been tested as an therapy in a number of cancer clinical trials. Although some antitumor responses reported, so far the majority studies failed to show significant CpG. Here we showed route administration is critical activity intravenous (i.v.) injection was capable inducing activation expansion tumor antigen-specific T cells, most these activated migrate...
Background Despite approval of immunotherapy for a wide range cancers, the majority patients fail to respond or relapse following initial response. These failures may be attributed immunosuppressive mechanisms co-opted by tumor cells. However, it is challenging use conventional methods systematically evaluate potential intrinsic factors act as immune regulators in with cancer. Methods To identify non-responders cancer an unbiased manner, we performed genome-wide CRISPR screens and integrated...
Protein arginine methyltransferases (PRMT) are a widely expressed class of enzymes responsible for catalyzing methylation on numerous protein substrates. Among them, type I PRMTs generating asymmetric dimethylarginine. By controlling multiple basic cellular processes, such as DNA damage responses, transcriptional regulation, and mRNA splicing, contribute to cancer initiation progression. A PRMT inhibitor, GSK3368715, has been developed entered clinical trials solid hematologic malignancies....
Psychological distress can influence a student's decision-making process regarding whether to persist in their academic journey. This study examined the of achievement goals and imposter syndrome on female STEM college students’ psychological distress. It involved 395 participants, with 37.72% identifying as minoritized 44.05% first-generation students, who completed an online survey. Findings structural equation modeling indicated that higher levels mediated link between...
Abstract Summary: Graft copolymers were designed that self‐assemble into hydrogels mediated by the interaction of coiled‐coil peptide domains. A linear hydrophilic polymer HPMA was chosen as backbone, and forming peptides, covalently attached to formed grafts. Microrheology used evaluate self‐assembly graft hydrogels. The results revealed length number grafts per chain had a significant influence on gelation process. At least 4 heptads needed achieve association CD spectra copolymer...
OX40 agonist-based combinations are emerging as a novel avenue to improve the effectiveness of cancer immunotherapy. To better guide its clinical development, we characterized role pathway in tumor-reactive immune cells. We also evaluated combining agonists with targeted therapy combat resistance immunotherapy.Experimental Design: utilized patient-derived tumor-infiltrating lymphocytes (TILs) and multiple preclinical models determine direct effect anti-OX40 agonistic antibodies on CD8+ T...
Abstract A small cysteine-rich protein with antimicrobial activity was isolated from pokeweed (Phytolacca americana) seeds and purified to homogeneity. The inhibits the growth of several filamentous fungi gram-positive bacteria. highly basic, a pI higher than 10. entire amino acid sequence determined be homologous (AMP) Mirabilis jalapa. cDNA encoding P. americana AMP (Pa-AMP-1) chromosomal DNA containing gene were cloned sequenced. deduced shows presence signal peptide at terminus,...
Rheumatoid arthritis (RA) is associated with chronic inflammation. The suppression of inflammation key to the treatment RA. Glucocorticoids (GCs) are classical anti-inflammatory drugs several disadvantages such as poor water solubility and low specificity in body. These reasons for quick elimination side effects GCs vivo. Micelles ideal carriers delivery inflamed synovium. We set out improve targeting pharmacokinetic profiles by preparing a micelle system.In this study, natural chlosterol...
Abstract Although immunotherapy has achieved impressive durable clinical responses, many cancers respond only temporarily or not at all to immunotherapy. To find novel, targetable mechanisms of resistance immunotherapy, patient-derived melanoma cell lines were transduced with 576 open reading frames, exposed arrayed libraries 850 bioactive compounds, prior co-culture autologous tumor-infiltrating lymphocytes (TILs). The synergy between the targets and TILs induce apoptosis, inhibiting...
Abstract Personalized medicine holds tremendous promise for improving safety and efficacy of drug therapies by optimizing treatment regimens. Rapidly developed patient-derived xenografts (pdx) could be a helpful tool analyzing the effect drugs against an individual’s tumor growing in immunodeficient animal. Severe combined immunodeficiency (SCID) mice enable efficient vivo expansion vital cells generation personalized xenografts. However, they are not amenable to large-scale rapid screening,...
CD40, a member of the tumor necrosis factor receptor superfamily, is broadly expressed on antigen-presenting cells and other cells, including fibroblasts endothelial cells. Binding CD40 its natural ligand CD40L (CD154) triggers cytokine secretion, increased expression costimulatory molecules required for T-cell activation proliferation. However, to our knowledge, use agonistic antibodies boost adoptively transferred T in vivo has not been investigated. The purpose this study was determine...
(1) Background: A cesarean scar defect may cause localized inflammation of the endometrial tissue, and various researchers believe that presence a is associated with chronic endometritis. However, there no report on possible association between defects endometritis thus far. This study aimed to assess role having in person’s susceptibility (2) Methods: retrospective propensity-score-matched comprised 1411 patients were admitted Henan Provincial People’s Hospital China from 2020 2022. Based...
Abstract T cell-mediated immunotherapies are promising cancer treatments. However, most patients still fail to respond these therapies. The molecular determinants of immune resistance poorly understood. Here, we interrogated the role loss expression tumor suppressor, PTEN, in resistance. In preclinical studies, found that silencing PTEN cells inhibited killing and decreased cell trafficking into tumors. clinical observed tumors with had significantly less CD8+ infiltration than PTEN-present...