A5022580070- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Electrochemical Analysis and Applications
- SARS-CoV-2 detection and testing
- Analytical Chemistry and Sensors
- Molecular Junctions and Nanostructures
- 3D Printing in Biomedical Research
- Advanced Sensor and Energy Harvesting Materials
- Cellular Mechanics and Interactions
- Neuroscience and Neural Engineering
- Gold and Silver Nanoparticles Synthesis and Applications
- RNA and protein synthesis mechanisms
- Advancements in Battery Materials
- Cancer Genomics and Diagnostics
- Electrochemical sensors and biosensors
- Advanced Chemical Sensor Technologies
- DNA and Nucleic Acid Chemistry
- Electrocatalysts for Energy Conversion
- Advanced Photocatalysis Techniques
- Advanced battery technologies research
- Anodic Oxide Films and Nanostructures
- Nanocluster Synthesis and Applications
- Nanomaterials for catalytic reactions
- Advanced Biosensing Techniques and Applications
- Hydrogen Storage and Materials
Northwestern University
2022-2025
Institute of Advanced Study in Science and Technology
2024
University of Toronto
2011-2021
3M (United States)
2018
Toronto Public Health
2018
Pusan National University
2006-2014
Gwangju Institute of Science and Technology
2008
Incheon National University
2008
Dong-A University
2007
Progress toward the development of minimally invasive liquid biopsies disease is being bolstered by breakthroughs in analysis circulating tumor DNA (ctDNA): released from cancer cells into bloodstream. However, robust, sensitive, and specific methods detecting this emerging analyte are lacking. ctDNA has unique challenges, since it imperative to distinguish normal vs mutation-bearing sequences originating tumors. Here we report electrochemical detection mutated samples collected patients. By...
The development of new methods for direct viral detection using streamlined and ideally reagent-free assays is a timely important, but challenging, problem. challenge combatting the COVID-19 pandemic has been exacerbated by lack rapid effective to identify pathogens like SARS-CoV-2 on-demand. Existing gold standard nucleic acid-based approaches require enzymatic amplification achieve clinically relevant levels sensitivity are not typically used outside laboratory setting. Here, we report...
This communication reports a nanocatalyst-based electrochemical assay for proteins. Ultrasensitive detection has been achieved by signal amplification combined with noise reduction: the is amplified both catalytic reduction of p-nitrophenol to p-aminophenol gold-nanocatalyst labels and chemical p-quinone imine NaBH4; reduced employing an indium tin oxide electrode modified ferrocenyl-tethered dendrimer hydrophilic immunosensing layer.
Signal amplification and noise reduction are crucial for obtaining low detection limits in biosensors. Here, we present an electrochemical immunosensor which the signal is achieved using p-aminophenol (AP) redox cycling by hydrazine, level reduced implementing a background current. The obtained simple one-electrode, one-enzyme format. In sandwich-type heterogeneous mouse IgG, alkaline phosphatase label converts p-aminophenyl phosphate into AP 10 min. This generated electrooxidized at indium...
High-curvature electrodes facilitate rapid and sensitive detection of a broad class molecular analytes. These sensors have reached limits not attained using bulk macroscale materials. It has been proposed that immobilized DNA probes are displayed at high deflection angle on the sensor surface, which allows greater accessibility more efficient hybridization. Here we report first use all-atom dynamics simulations coupled with electrochemical experiments to explore single-stranded...
Abstract The analysis of circulating tumour nucleic acids (ctNAs) provides a minimally invasive way to assess the mutational spectrum tumour. However, effective and practical methods for analyzing this emerging class markers are lacking. Analysis ctNAs using sensor‐based approach has notable challenges, as it is vital differentiate from normal cells mutation‐bearing sequences tumours. Moreover, many genes related cancer have dozens different mutations. Herein, we report an electrochemical...
Cell morphology and geometry affect cellular processes such as stem cell differentiation, suggesting that these parameters serve fundamental regulators of biological within the cell. Hierarchical architectures featuring micro- nanotopographical features therefore offer programmable systems for differentiation. However, a limited number studies have explored effects hierarchical due to complexity fabricating with rationally tunable nanostructuring. Here, we report three-dimensional (3D)...
Abstract Circulating tumour nucleic acids (ctNAs) are released from tumours cells and can be detected in blood samples, providing a way to track tumors without requiring tissue sample. This “liquid biopsy” approach has the potential replace invasive, painful, costly biopsies cancer diagnosis management. However, very sensitive specific is required detect relatively low amounts of mutant sequences linked because they masked by high levels wild‐type sequences. review discusses high‐performance...
Exosomal PD-L1 (exoPD-L1) has recently received significant attention as a biomarker predicting immunotherapeutic responses involving the PD1/PD-L1 pathway. However, current technologies for exosomal analysis rely primarily on bulk measurements that do not consider heterogeneity found within subpopulations. Here, we present nanoscale cytometry platform NanoEPIC, enabling phenotypic sorting and exoPD-L1 profiling from blood plasma. We highlight efficacy of NanoEPIC in monitoring anti-PD-1...
Abstract Reagent‐free electronic biosensors capable of analyzing disease markers directly in unprocessed body fluids will enable the development simple & affordable devices for personalized healthcare monitoring. Here we report a powerful and versatile nucleic acid‐based reagent‐free sensing system. The signal transduction is based on kinetics an electrode‐tethered molecular pendulum—a rigid double stranded DNA with one strands displaying analyte‐binding aptamer other featuring redox...
Fast, sensitive nucleic acid sensors that enable direct detection of bacteria and diagnosis infectious disease would offer significant advantages over existing approaches employ enzymatic amplification acids. We have developed chip-based microelectrodes are highly effective for bacterial shown they can capture permit the analysis large slow moving mRNA targets. Here, we explore new to tune their analytical sensitivity investigate effect sensor size, material composition, probe density on...
Abstract In living systems, interfacial molecular interactions control many biological processes. New stimuli‐responsive strategies are desired to provide versatile model systems that can regulate cell behavior in vitro. Described here potential‐responsive surfaces adhesion and release as well stem differentiation. Cell be modulated dynamically by applying negative positive potentials functionalized with tailored monolayers. This process alters morphology ultimately controls the fate of...
Continuous measurement of proteins in vivo is important for real-time disease management and prevention. Implantable sensors monitoring small molecules such as glucose have been available more than a decade. However, analysis remains an unmet need because the lower physiological levels require that high affinities, which are linked to long complexation half-lives ( t 1/2 ~20 hours) slow equilibration when concentrations decrease. We report active-reset by use high-frequency oscillations...
Nanomaterials have extensive applications in the development of sensitive biosensors, but influence their specific structural properties remains unclear. This work presents a platform that can provide mechanistic insight into how nanostructured electrodes improve performance electrochemical biosensors. We designed nanoelectrodes with sub-10 nm spike features through combination top-down lithography and solution-based synthesis. These anisotropic structures facilitated rapid...