A5007991064- Microbial Community Ecology and Physiology
- Wastewater Treatment and Nitrogen Removal
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Fuel Cells and Bioremediation
- Gut microbiota and health
- Ammonia Synthesis and Nitrogen Reduction
- Environmental DNA in Biodiversity Studies
- Antibiotic Resistance in Bacteria
- Underwater Acoustics Research
- Building Energy and Comfort Optimization
- Bacterial biofilms and quorum sensing
- Marine and coastal ecosystems
- Algal biology and biofuel production
- Network Security and Intrusion Detection
- Electrocatalysts for Energy Conversion
- Aquatic Ecosystems and Phytoplankton Dynamics
- Vibration and Dynamic Analysis
- Advanced oxidation water treatment
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Constructed Wetlands for Wastewater Treatment
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Underwater Vehicles and Communication Systems
- Cell Image Analysis Techniques
Northwestern University
2016-2023
Wright-Patterson Air Force Base
2014
U.S. Air Force Institute of Technology
2014
Naval Undersea Warfare Center
2002
Coupled aerobic–anoxic nitrous decomposition operation (CANDO) is a promising emerging bioprocess for wastewater treatment that enables direct energy recovery from nitrogen (N) in three steps: (1) ammonium oxidation to nitrite; (2) denitrification of nitrite oxide (N2O); and (3) N2O conversion N2 with generation. However, CANDO does not currently target phosphorus (P) removal. Here, we demonstrate denitrifying polyphosphate-accumulating organism (PAO) enrichment cultures are capable...
Wastewater treatment plants (WWTPs) are known to harbor antibiotic resistance genes (ARGs) that disseminated into the environment via effluent. However, few studies have compared abundance, mobilization and selective pressures for ARGs in WWTPs as a function of variations secondary bioprocesses. We used shotgun metagenomics provide comprehensive analysis ARG composition, relationship mobile genetic elements co-occurrences with production (APGs) throughout two full-scale municipal WWTPs, one...
Current gene editing approaches in eukaryotic cells are limited to single base edits or small DNA insertions and deletions, remain encumbered by unintended permanent effects significant challenges the delivery of large cargo. Here we describe Splice Editing, a generalizable platform correct transcripts situ programmable insertion replacement RNA segments. By combining CRISPR-mediated targeting with endogenous cellular RNA-splicing machinery, Editing enables efficient, precise, large-scale...
Intensively managed land increases the rate of nutrient and particle transport within a basin, but impact these changes on microbial community assembly patterns at basin scale is not yet understood. The objective this study was to investigate how landscape connectivity dispersal impacts diversity in an agricultural-dominated watershed. We characterized soil, sediment water communities along Upper Sangamon River Illinois—a 3600 km2 watershed strongly influenced by human activity, especially...
Abstract Morphological parameters are commonly used to predict transport and metabolic kinetics in biofilms. Yet, quantification of biofilm morphology remains challenging because imaging technology limitations lack robust analytical approaches. We present a novel set image analysis techniques estimate internal porosity, pore size distributions, network connectivity depth 1 mm at resolution 10 µm exhibiting both heterotrophic nitrifying activities. Optical coherence tomography (OCT) scans...
In this work, we demonstrate a combined bioelectrochemical and inorganic catalytic system for resource recovery from wastewater. We designed microbial peroxide producing cell (MPPC) hydrogen (H2O2) production used bioelectrochemically derived H2O2 as green oxidant sulfoxidation, an industrial reaction chemical synthesis oxidative desulfurization of transportation fuels. operated MPPC equipped with gas diffusion electrode cathode six months, achieving peak current density above 1.4 mA cm–2...
Abstract Attached growth bioprocesses that use biofilms to remove organic matter or nutrients from wastewater are known harbor antibiotic resistance genes (ARGs). Biofilms in these processes spatially heterogeneous, but little is about depth stratification of ARGs complex, mixed culture biofilms. To address this knowledge gap, we used an experimental approach combining cryosectioning and quantitative polymerase chain reaction quantify the spatial distribution three ( sul1, ermB, qnrS ) class...
While the impacts of soil moisture on microbiome diversity and composition are well characterized, influence hydrological regime has been overlooked. As precipitation patterns altered by climate change, understanding impact hydrology community structure function is critical. In this work, water level was continuously monitored for over a year in Midwestern prairie-wetland at 10 cm depth increments up to 120 locations. We analyzed edaphic factors cores collected from unique spatially...
Asset management is a systems approach to managing an organization's portfolio of real property assets. Although range activities exist help manage these assets, understanding facility energy consumption critical component from life-cycle decision-making perspective. Since national concern for most countries, this has resulted in various initiatives regarding conservation measures. To better understand the factors affecting facilities, and meteorological data covering 22 years were collected...
Abstract Introducing hydrogen into the existing natural gas pipeline network has been proposed as a way of decreasing greenhouse (GHG) effects from combustion gas. However, is known to degrade strength many alloys through embrittlement (HE). Therefore, it important carefully define maximum allowable pressure concentration for each alloy avoid detrimental material. In this study, testing was carried out evaluate susceptibility selected via controlled exposure methane blended with different...
Microalgae accumulate lipids in response to nutrient deprivation, and these are a biodiesel fuel stock. Algal cultivation with secondary wastewater effluent is one proposed platform for biofuel production, which provides nutrients algae while further polishing effluent. bioreactors were tested using feast-famine feeding regiment simulated evaluate the effects on lipid content algal community structure. polycultures inoculated into reactors fed synthetic at pH 7.5 9 operated under (N, P, BOD)...
For an acoustic transducer mounted on the exterior of a submerged hull structure, effect dynamic mobility upon directivity patterns is subject considerable interest to array design engineers. Cylindrical geometry, mounting point impedance, and structural dispersion are possible significant factors in beam pattern distortion. This analytical study utilizes fundamental fluid/structure interaction model cylindrical shells investigate role vibrations induced by reactive excitation element. It...
Acoustic testing was conducted to characterize the self-noise state of Odyssey II class autonomous underwater vehicles (AUVs). The tests were at Naval Undersea Warfare Center Division Newport with additional personnel from Massachusetts Institute Technology and Ocean Acoustical Services Instrumentation Systems. Measurements sound pressure structural acceleration made various points on interior exterior a vehicle while it exercised actuators in manner representative actual operating...
Abstract Recent findings show that a subset of bacteria affiliated with Nitrospira , genus known for its importance in nitrite oxidation biological nutrient removal applications, are capable co mplete amm onia ox idation (comammox) to nitrate. Early reports suggested they were absent or present low abundance most activated sludge processes, and thus likely functionally irrelevant. Here we the accumulation comammox nitrifying sequencing batch reactor operated at dissolved oxygen (DO)...