Effective re-endothelialization is critical for the use of decellularized scaffolds ex vivo lung engineering. Current approaches yield insufficiently re-endothelialized that haemorrhage and become thrombogenic upon implantation. Herein, gravity-driven seeding coupled with bioreactor culture facilitated widespread distribution engraftment endothelial cells throughout rat scaffolds. Initially, human umbilical vein were seeded into pulmonary artery by either gravity-driven, variable flow...

Hydrogels serve as three-dimensional scaffolds whose composition can be customized to allow attachment and proliferation of several different cell types. Extracellular matrix-derived hydrogels are considered close replicates the tissue microenvironment. They for in vitro engineering a useful tool study cell-scaffold interaction. The aim present was analyze effect adipose-derived stromal/stem cells (ASCs) decellularized adipose tissue-derived (DAT) hydrogel interaction on ASC morphology,...

A challenge in tissue engineering biomimetic models for studying angiogenesis is building the physiological complexity of real microvascular networks. Our laboratory recently introduced rat mesentery culture model as an ex vivo experimental platform investigating multicellular dynamics involved within intact The objective this study was to compare endothelial cell phenotypes along capillary sprouts cultured networks phenotypes. For Day 3 (Ex Vivo) tissues, adult tissues were three days media...

Abstract While decellularized adipose tissue (DAT) has potential as an “off‐the‐shelf” biomaterial product for regenerative medicine, it remains to be determined if donor‐source body mass index (BMI) impacts the functionality of DAT. This study set out comparatively characterize lean versus overweight/obese‐donor derived DAT hydrogel based on proteome and analyze their respective effects stromal/stem cell (ASC) viability, differentiation in vitro. Decellularized from (lDAT) overweight/obese...

Abstract Objective Emerging areas of vascular biology focus on lymphatic/blood vessel mispatterning and the regulation endothelial cell identity. However, a fundamental question remains unanswered: Can lymphatic vessels become blood in adult tissues? Leveraging novel tissue culture model, objective this study was to track fate over time course microvascular network remodeling. Methods Cultured Wistar rat mesenteric tissues were labeled with BSI‐lectin time‐lapse images captured five days...

Intra-articular fractures (IAF) result in significant and prolonged inflammation, increasing the chances of developing post-traumatic osteoarthritis (PTOA). Interleukin-one beta (IL-1β) Tumor Necrosis Factor-alpha (TNF-α) are key inflammatory factors shown to be involved osteochondral degradation following IAF. As such, use targeted biologics such as Infliximab (INX), a TNF-α inhibitor, Anakinra (ANR), an interleukin-one (IL-1) receptor antagonist (IL1RA), may protect against PTOA by damping...

Angiogenesis, defined as the growth of new blood vessels from pre-existing vessels, involves endothelial cells, pericytes, smooth muscle immune and coordination with lymphatic nerves. The multi-cell, multi-system interactions necessitate investigation angiogenesis in a physiologically relevant environment. Thus, while use vitro cell-culture models have provided mechanistic insights, common critique is that they do not recapitulate complexity associated microvascular network. objective this...

Abstract An emerging challenge in tissue engineering biomimetic models is recapitulating the physiological complexity associated with real tissues. Recently, our laboratory introduced rat mesentery culture model as an ex vivo experimental platform for investigating multi-cellular dynamics involved angiogenesis within intact microvascular network using time-lapse imaging. A critical question remains whether vessels maintain their functionality. The objective of this study was to determine...

Microvascular remodeling, or angiogenesis, plays a central role in multiple pathological conditions, including cancer, diabetes, and ischemia. Tissue-engineered vitro models have emerged as tools to elucidate the mechanisms that drive angiogenic process. However, major challenge with model development is recapitulating physiological complexity of real microvascular networks, incorporation entire vascular tree hemodynamics. This study establishes bioreactor system incorporates networks flow...

Volumetric muscle loss (VML)-defined as the irrecoverable of skeletal tissue with associated persistent functional deficits-is among most common and highly debilitating combat-related extremity injuries. This is particularly true in cases severe polytrauma wherein multiple extremities may be involved a result high energy wounding mechanisms. As such, significant investment effort has been made toward developing clinically viable intervention capable restoring form function affected...

The use of a rehabilitation approach that promotes regeneration has the potential to improve efficacy pro-regenerative therapies and maximize functional outcomes in treatment volumetric muscle loss (VML). An adjunct antifibrotic could further enhance gains by reducing fibrotic scarring. This study aimed evaluate synergistic effects losartan, an pharmaceutical, paired with voluntary wheel running strategy minced graft (MMG) therapy rodent model VML. animals were randomly assigned into four...

Ex vivo biomimetic models are valuable for investigating the underlying mechanisms of angiogenesis. Common currently in use include 2D or 3D culture systems and microfluidic devices. However, these limited by their lack complexity compared to intact‐tissue scenarios. Based on an alternative top‐down approach, our laboratory has recently introduced rat mesentery model as a tool investigate cell‐cell interactions during angiogenesis viable, intact microvascular networks. The objective this...

Angiogenesis, defined as the growth of new blood vessels from pre-existing vessels, involves endothelial cells, pericytes, smooth muscle immune and coordination with lymphatic nerves. The multi-cell, multi-system interactions necessitate investigation angiogenesis in a physiologically relevant environment. Thus, while use vitro cell-culture models have provided mechanistic insights, common critique is that they do not recapitulate complexity associated microvascular network. objective this...

An emerging challenge in tissue engineering biomimetic models is recapitulating the physiological complexity associated with real tissues. Recently, our laboratory introduced rat mesentery culture model as an ex vivo experimental platform for investigating multi‐cellular dynamics involved angiogenesis within intact microvascular network using time‐lapse imaging. A critical question remains whether vascular smooth muscle cells maintain their functionality. The objective of this study was to...

A challenge in tissue engineering biomimetic models for studying angiogenesis is building the physiological complexity of real microvascular networks. Utilizing an alternative top‐down approach, our laboratory recently introduced rat mesentery culture model as ex vivo experimental platform investigating multi‐cellular dynamics involved within intact network. However, a current limitation that microvessels are not perfused. The objective this study was to demonstrate feasibility perfusing...