A5043197401- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Nitric Oxide and Endothelin Effects
- Neurological Disease Mechanisms and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Neuroscience and Neural Engineering
- Barrier Structure and Function Studies
- Photoreceptor and optogenetics research
- Alzheimer's disease research and treatments
- Acute Ischemic Stroke Management
- Tryptophan and brain disorders
- Neurotransmitter Receptor Influence on Behavior
- Metabolomics and Mass Spectrometry Studies
- NF-κB Signaling Pathways
- Estrogen and related hormone effects
- Neurogenesis and neuroplasticity mechanisms
- Neuropeptides and Animal Physiology
- Menopause: Health Impacts and Treatments
- Glioma Diagnosis and Treatment
- Cannabis and Cannabinoid Research
- Renin-Angiotensin System Studies
- Transcranial Magnetic Stimulation Studies
Tulane University
2015-2024
University of California, Los Angeles
2008-2014
Universidad de Cantabria
2004-2011
Instituto de Biomedicina y Biotecnología de Cantabria
2009
Unidades Centrales Científico-Técnicas
2008
Consejo Superior de Investigaciones Científicas
2008
Universidad de León
2001
During neocortical development, neurons exhibit highly synchronized patterns of spontaneous activity, with correlated bursts action potential firing dominating network activity. This early activity is eventually replaced by more sparse and decorrelated cortical neurons, which modeling studies predict a state that better suited for efficient neural coding. The precise time course mechanisms this crucial transition in have not been characterized vivo. We used vivo two-photon calcium imaging...
What is the neuroanatomical basis for decline in brain function that occurs during normal aging? Previous postmortem studies have blamed it on a reduction spine density, though results remain controversial and dynamics were not assessed. We used chronic vivo two-photon imaging of dendritic spines axonal boutons somatosensory cortex up to 1 year thy1 GFP mice test hypothesis aging associated with alterations synaptic dynamics. find density en passant (EPBs) pyramidal cells increases...
Imaging techniques are becoming increasingly important in the study brain function. Among them, two-photon laser scanning microscopy has emerged as an extremely useful method, because it allows of live intact brain. With appropriate preparations, this technique observation same cortical area chronically, from minutes to months. In video, we show a preparation for chronic vivo imaging using microscopy. This was initially pioneered by Dr. Karel Svoboda, who is now Howard Hughes Medical...
Estrogen is formed by the enzyme aromatase (CYP19A1) and signals via three identified receptors ERα (ESR1), ERß (ESR2), G protein-coupled estrogen receptor (GPER). Understanding relative contribution of each to estrogenic signaling may elucidate disparate effects this sex hormone across tissues, recent developments in PCR technology allow absolute quantification direct comparison multiple targets. We hypothesized that approach would reveal tissue- sex-specific differences mRNA.
Mitochondrial and glycolytic energy pathways regulate the vascular functions. Aging impairs cerebrovascular function increases risk of stroke cognitive dysfunction. The goal our study is to characterize impact aging on brain microvascular energetics. We measured oxygen consumption extracellular acidification rates freshly isolated microvessels (BMVs) from young (2–4 months) aged (20–22 C57Bl/6 male mice. Cellular ATP production in BMVs was predominantly dependent oxidative phosphorylation...
Changes in dendritic spine turnover are a major mechanism of experience-dependent plasticity the adult neocortex. Dendritic may also contribute to functional recovery after stroke, but that setting its expression be complicated by alterations local tissue perfusion, especially around infarct. Using Thy-1 GFP-M mice, we simultaneously recorded long-term dynamics apical dendrites from layer 5 pyramidal cells and blood flow surrounding capillaries with vivo two-photon microscopy peri-infarct...
Mice with insulin receptor (IR)–deficient astrocytes (GFAP-IR knockout [KO] mice) show blunted responses to and reduced brain glucose uptake, whereas IR-deficient disturbed mitochondrial glucose. While exploring the functional impact of function in astrocytes, we observed that GFAP-IR KO mice uncoupling blood flow uptake. Since higher levels reactive oxidant species (ROS), this leads stimulation hypoxia-inducible factor-1α and, consequently, vascular endothelial growth factor angiogenic...
Risk factors contributing to dementia are multifactorial. Accumulating evidence suggests a role for pathogens as risk factors, but data is largely correlative with few causal relationships. Here, we demonstrate that intermittent murine cytomegalovirus (MCMV) infection of mice, alters blood brain barrier (BBB) permeability and metabolic pathways. Increased basal mitochondrial function observed in microvessels cells (BMV) exposed MCMV accompanied by elevated levels superoxide. Further, mice...
The mode of action antidepressant drugs may be related to mechanisms monoamines receptor adaptation, including serotonin 5-HT(4) subtypes. Here we investigated the effects repeated treatment with selective reuptake inhibitor fluoxetine for 21 days (5 and 10 mg/kg, p.o., once daily) on sensitivity receptors by using autoradiography, adenylate cyclase assays extracellular recording techniques in rat brain. Fluoxetine decreased density binding CA1 field hippocampus as well several areas...
When stroke or traumatic brain injury lead to cortical damage, how do surviving neurons rewire the restore lost functionalities? Several Golgi studies have argued for de novo growth and branching of dendrites pyramidal in spared hemisphere, but results could not always be replicated. Functional imaging humans rodents suggest that significant neuronal plasticity occurs areas surrounding lesion, whether dendritic rearrangements occur there has been less well studied, especially after stroke....
Effective re-endothelialization is critical for the use of decellularized scaffolds ex vivo lung engineering. Current approaches yield insufficiently re-endothelialized that haemorrhage and become thrombogenic upon implantation. Herein, gravity-driven seeding coupled with bioreactor culture facilitated widespread distribution engraftment endothelial cells throughout rat scaffolds. Initially, human umbilical vein were seeded into pulmonary artery by either gravity-driven, variable flow...
Sustained inflammation and matrix metalloproteinase (MMP) activation contribute to vascular occlusive/proliferative disorders. Interleukin-17 (IL-17) is a proinflammatory cytokine that signals mainly via TRAF3 Interacting Protein 2 (TRAF3IP2), an upstream regulator of various critical transcription factors, including AP-1 NF-κB. Reversion inducing cysteine rich protein with kazal motifs (RECK) membrane-anchored MMP inhibitor. Here we investigated whether IL-17A/TRAF3IP2 signaling promotes...
Imaging techniques are becoming increasingly important in the study brain function. Among them, two-photon laser scanning microscopy has emerged as an extremely useful method, because it allows of live intact brain. With appropriate preparations, this technique observation same cortical area chronically, from minutes to months. In video, we show a preparation for chronic vivo imaging using microscopy. This was initially pioneered by Dr. Karel Svoboda, who is now Howard Hughes Medical...
Most stroke survivors exhibit a partial recovery from their deficits. This presumably occurs because of remapping lost capabilities to functionally related brain areas. Functional imaging studies suggest that in the contralateral uninjured cortex might represent transient stage compensatory plasticity. Some postmortem have also shown cortical lesions, including stroke, can trigger dendritic plasticity hemisphere, but data are controversial. We used longitudinal vivo two-photon microscopy...
Hydrogen sulfide (H2S) is an endogenous gaseous molecule formed from L-cysteine in vascular tissue. In the present study, cardiovascular responses to H2S donors Na2S and NaHS were investigated anesthetized rat. The intravenous injections of 0.03-0.5 mg/kg produced dose-related decreases systemic arterial pressure heart rate, at higher doses cardiac output, pulmonary pressure, resistance. infusion studies show that resistance are well-maintained, reversible. Decreases rate not blocked by...
Abstract Differentially expressed (DE) proteins in the cortical microvessels (MVs) of young, middle-aged, and old male female mice were evaluated using discovery-based proteomics analysis (> 4,200 quantified proteins/group). Most DE 90%) showed no significant differences between sexes; however, some showing sexual MVs decreased from young (8.3%), to middle-aged (3.7%), (0.5%) mice. Therefore, we combined data for age-dependent comparisons but noted sex examination. Key involved oxidative...
The ability to image the cerebral vasculature (from large vessels capillaries) and record blood flow dynamics in intact brain of living rodents is a powerful technique. Using vivo 2-photon microscopy through cranial window it possible fluorescent dyes injected intravenously. This permits one cortical also obtain measurements flow. technique was originally developed by David Kleinfeld Winfried Denk. method can be used study during or after ischemia, neurodegenerative disorders, tumors, normal...