A5040681808- Cytomegalovirus and herpesvirus research
- Neuroinflammation and Neurodegeneration Mechanisms
- SARS-CoV-2 and COVID-19 Research
- CRISPR and Genetic Engineering
- Toxoplasma gondii Research Studies
- Tryptophan and brain disorders
- Herpesvirus Infections and Treatments
- Genetics, Bioinformatics, and Biomedical Research
- COVID-19 Clinical Research Studies
- COVID-19 epidemiological studies
- Biotechnology and Related Fields
- Mitochondrial Function and Pathology
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Gold and Silver Nanoparticles Synthesis and Applications
- Anodic Oxide Films and Nanostructures
- Extracellular vesicles in disease
- Viral gastroenteritis research and epidemiology
Tulane University
2020-2025
Risk factors contributing to dementia are multifactorial. Accumulating evidence suggests a role for pathogens as risk factors, but data is largely correlative with few causal relationships. Here, we demonstrate that intermittent murine cytomegalovirus (MCMV) infection of mice, alters blood brain barrier (BBB) permeability and metabolic pathways. Increased basal mitochondrial function observed in microvessels cells (BMV) exposed MCMV accompanied by elevated levels superoxide. Further, mice...
CRISPR/ Diagnostics: A portable lab for everyone Professor Kevin J. Zwezdaryk and Chandler H. Monk discuss CRISPR diagnostics, focusing on the development of a accessible to everyone. The COVID pandemic highlighted importance simple, safe, easy-to-use, reliable diagnostic platforms. Family members all ages used simple sample collection method provide an easily understandable result – whether they had or did not have COVID. At-home antigen detection tests positively altered public health...
Abstract Background Cytomegalovirus (CMV), is the most common congenital infection in United States. Congenital CMV (cCMV) affects 3-6 per 1000 live born infants yearly. Up to 80% of cCMV cases are asymptomatic but can result long term sequelae, including sensorineural hearing loss. Despite improved diagnostic strategies and therapeutics, universal screening programs not standard care. Our laboratory addressing this gap by designing a novel isothermal CRISPR-based point-of-care assay for...
BACKGROUND. Circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA may represent a more reliable indicator of infection than nasal RNA, but quantitative reverse transcription PCR (RT-qPCR) lacks diagnostic sensitivity for blood samples.
Human cytomegalovirus (HCMV) is a near ubiquitous herpesvirus that relies on host cell metabolism for efficient replication. Although it has been shown HCMV requires functional mitochondria replication, unknown whether mitochondrial targeted pharmacological agents can be repurposed as antivirals. Here we report treatment with drugs targeting the electron transport chain (ETC) complexes inhibit Addition of rotenone, oligomycin, antimycin and metformin resulted in decreased titers vitro,...
Despite decades of research, human cytomegalovirus (CMV) continues to contribute significant morbidity and mortality in transplant settings remains the leading cause viral congenital infections. Clinical diagnosis CMV infection and/or reactivation under these is completed using real time quantitative polymerase chain reaction (RT-qPCR). This assay performs well but hampered by poor sensitivity a lack standardization among testing facilities. A point-of-care rapid diagnostic determine viremia...
Abstract Purpose of Review Metabolic rewiring the host cell is required for optimal viral replication. Human cytomegalovirus (HCMV) has been observed to manipulate numerous mitochondrial functions. In this review, we describe strategies and targets HCMV uses control different aspects function. Recent Findings The mitochondria are instrumental in meeting biosynthetic bioenergetic needs This achieved through altered metabolism signaling pathways. Morphological changes mediated biogenesis...
Serologic testing of residual blood samples from 812 children a hospital in New Orleans, LA, between March and May 2020, demonstrated SARS-CoV-2 seroprevalence 6.8% based on S N protein IgG; Black Hispanic children, living zip codes with lower household incomes were over-represented.
athogens are microorganisms, including viruses, bacteria, and fungi, which invade our bodies make us sick.Many of these pathogens cause transmittable diseases; therefore, their early accurate detection is essential not only for choosing effective treatments, but also controlling the spread disease preventing pandemics.On top this, modern medical practices, such as organ transplantations new targeted treatments cancers, have led to a growing number people with weak immune systems whose lives...
SUMMARY Risk factors contributing to dementia are multifactorial. Pathogens as risk for is largely correlative with few causal relationships. Here, we demonstrate that intermittent cytomegalovirus (CMV) infection in mice, mimicking human chronic and reactivation/reinfection events, alters blood brain barrier (BBB) metabolic pathways. An increase basal mitochondrial function observed microvasculature endothelial cells (BMEC) at 12 months post but not earlier time points accompanied by...