A5010179838- Bone fractures and treatments
- Muscle Physiology and Disorders
- Tissue Engineering and Regenerative Medicine
- Osteoarthritis Treatment and Mechanisms
- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Knee injuries and reconstruction techniques
- Electrospun Nanofibers in Biomedical Applications
- Muscle activation and electromyography studies
- Reconstructive Surgery and Microvascular Techniques
- 3D Printing in Biomedical Research
- Periodontal Regeneration and Treatments
- Colorectal Cancer Surgical Treatments
- Hernia repair and management
- Sports injuries and prevention
- Orthopaedic implants and arthroplasty
- Orthopedic Surgery and Rehabilitation
- Total Knee Arthroplasty Outcomes
- Myofascial pain diagnosis and treatment
- Nerve injury and regeneration
- Wound Healing and Treatments
- Advanced Sensor and Energy Harvesting Materials
- Graphene and Nanomaterials Applications
- Orthopedic Infections and Treatments
- Shoulder Injury and Treatment
Uniformed Services University of the Health Sciences
2020-2024
Defense Health Agency
2020-2024
Walter Reed National Military Medical Center
2019-2023
Center for Excellence in Education
2023
Montgomery General Hospital
2023
United States Army Institute of Surgical Research
2016-2020
United States Army
2016
Georgia Institute of Technology
2007-2016
Abstract Skeletal muscle possesses a remarkable capacity to regenerate when injured, but confronted with major traumatic injury resulting in volumetric loss (VML), the regenerative process consistently fails. The of tissue and function from VML has prompted development suite therapeutic approaches these strategies have proceeded without comprehensive understanding molecular landscape that drives response. Herein, we administered an established rodent model monitored evolution healing...
Volumetric muscle loss (VML) resulting from extremity trauma presents chronic and persistent functional deficits which ultimately manifest disability. Acellular biological scaffolds, or decellularized extracellular matrices (ECMs), embody an ideal treatment platform due to their current clinical use for soft tissue repair, off-the-shelf availability, zero autogenous donor burden. ECMs have been reported promote skeletal remodeling in small large animal models of VML injury, this conclusion...
The traumatic loss of muscle tissue, defined as volumetric (VML) injury, has no definitive therapy. purposes this study were: (1) to develop a porcine model VML and (2) investigate autologous minced grafts (1-mm pieces muscle) potential therapeutic. Minced were evaluated because they have promoted fiber regeneration functional recovery in rat models do not require US Food Drug Administration approval for clinical use.In 5 female Yorkshire-cross pigs, ≈5 g (≈20%) tissue was excised from the...
Volumetric muscle loss (VML) occurs following significant traumatic injury or surgical removal of skeletal muscle, resulting in nonrecoverable tissue and long-term dysfunction. Perhaps less recognized is that VML injuries inherently disrupt the neuromuscular unit, fiber denervation presumptive motor unit rearrangement, expansion, and/or loss. To characterize neural dysfunction we quantified motoneuron axotomy, efforts to understand how this relates temporal coordination morphological...
Minced muscle autografting mediates de novo myofiber regeneration and promotes partial recovery of neuromuscular strength after volumetric loss injury (VML). A major limitation this approach is the availability sufficient donor tissue for treatment relatively large VMLs without inducing site morbidity. This study evaluated a laminin-111 supplemented hyaluronic acid based hydrogel (HA+LMN) as putative myoconductive scaffolding to be co-delivered with minced grafts. In rat tibialis anterior...
The deleterious impact of concomitant muscle injury on fracture healing and limb function is commonly considered part the natural sequela orthopedic trauma. Recent reports suggest that heightened inflammation in surrounding traumatized musculature a primary determinant healing. Relatedly, there are emerging potential therapeutic approaches for severe trauma (e.g., volumetric loss [VML] injury), such as autologous minced grafts (1 mm3 pieces muscle; GRAFT), can partially prevent chronic...
Heightened local inflammation due to muscle trauma or disease is associated with impaired bone regeneration. We hypothesized that FK506, an FDA approved immunomodulatory compound neurotrophic and osteogenic effects, will rescue the early phase of fracture healing which by concomitant in male (~4 months old) Lewis rats. FK506 (1 mg/kg; i.p.) saline was administered systemically for 14 days after endogenously tibia osteotomy created fixed intermedullary pin, overlying tibialis anterior (TA)...
BACKGROUND Traumatic muscle loss (i.e., volumetric [VML] injury) impairs adjacent fracture healing but is often left untreated. A promising therapy for this application a decellularized extracellular matrix (ECM) because of their capacity to regenerate vascularized tissue bed. This study tested the hypothesis that repair VML concomitant with small intestine submucosa (SIS)–ECM improves musculoskeletal healing. METHODS In male Lewis rats (~375 g), 3-mm segmental bone defect (SBD) was created...
The fabrication of tissue-engineered constructs clinically relevant sizes continues to be plagued by poor nutrient transport the interior construct. Consequences mass transfer construct core include large gradients in cell viability and matrix deposition, as well inadequate mechanical functionality. Prior literature has shown that embedded microfluidic channels offer potential control spatial temporal presentation hydrodynamic chemical cues within developing tissue toward improved transfer....
Minced muscle grafts (MG) promote de novo fiber regeneration and neuromuscular strength recovery in small large animal models of volumetric loss. The most noteworthy limitation this approach is its reliance on a finite supply donor tissue. To address shortcoming, study sought to evaluate micronized acellular urinary bladder matrix (UBM) as scaffolding vivo expansion MG therapy rat model. Rats received loss injuries the tibialis anterior their left hind limb which were either untreated or...
The development of tissue engineered osteochondral units has been slowed by a number technical hurdles associated with recapitulating their heterogeneous nature ex vivo. Subsequently, numerous approaches respect to cell sourcing, scaffolding composition, and culture media formulation have pursued, which led high variability in outcomes ultimately the lack consensus bioprocessing strategy. As such, objective this study was standardize design process focusing on differentially supporting...
Extremity injuries occur frequently during warfare. While traditionally treated in definitive clinics, the future battlefield is projected to be different a variety of ways, and there will likely shift towards prolonged field care (PFC) for treating extremity traumas. PFC defined as medical that applied beyond "doctrinal planning time-lines" by tactical practitioner order decrease patient mortality morbidity. At present, an urgent need develop biologically focused technologies setting....
Volumetric muscle loss (VML) represents a devastating extremity injury which leads to chronic functional deficits and disability is unrecoverable through normal healing pathways. When left untreated, the VML pathophysiology creates many challenges towards successful treatment, such as altered residual architecture, excessive fibrosis, contracture(s). As such, innovative approaches technologies are needed prevent or reverse these adverse sequelae. Development of rationally designed...
Intra-articular fractures (IAF) result in significant and prolonged inflammation, increasing the chances of developing post-traumatic osteoarthritis (PTOA). Interleukin-one beta (IL-1β) Tumor Necrosis Factor-alpha (TNF-α) are key inflammatory factors shown to be involved osteochondral degradation following IAF. As such, use targeted biologics such as Infliximab (INX), a TNF-α inhibitor, Anakinra (ANR), an interleukin-one (IL-1) receptor antagonist (IL1RA), may protect against PTOA by damping...
The delivery of alternative myogenic cell sources to enhance the efficacy minced muscle grafts (MG) for treatment volumetric loss (VML) injuries is a promising strategy overcome demand on muscle-derived donor tissue that currently limits translation this therapy. Using rat model VML, bone marrow mononuclear cells (BMNCs) were evaluated their ability directly contribute de novo fiber regeneration by transplanting MG in collagen carrier at dose 50% VML injury both with and without concomitant...
Volumetric muscle loss (VML)-defined as the irrecoverable of skeletal tissue with associated persistent functional deficits-is among most common and highly debilitating combat-related extremity injuries. This is particularly true in cases severe polytrauma wherein multiple extremities may be involved a result high energy wounding mechanisms. As such, significant investment effort has been made toward developing clinically viable intervention capable restoring form function affected...
Composite tissue injuries (CTIs) in extremities include segmental bone defects (SBDs) and volumetric muscle loss. The objective of this study was to determine if skeletal autografting with minced grafts (MMGs) could improve healing an SBD function a porcine CTI model that includes adjacent loss injury. Adult Yucatan Minipigs were stratified into three groups including specimens isolated SBD, (CTI), treated MMG (CTI + MMG). Bone quantified serial x-rays postmortem computed tomography...