A5094305201- Mosquito-borne diseases and control
- Influenza Virus Research Studies
- Viral Infections and Vectors
- HIV Research and Treatment
- vaccines and immunoinformatics approaches
- Respiratory viral infections research
- Insect and Pesticide Research
Center for Research and Advanced Studies of the National Polytechnic Institute
2024-2025
Nuclear transport of proteins larger than 60 kDa occurs via energy-dependent active transport, whereas smaller diffuse into the nucleus through nuclear pore complexes passive transport. Although dengue virus (DENV) replication cycle primarily takes place in cytoplasm, capsid protein and non-structural 5 (NS5) are imported a localization sequence-dependent mechanism. However, given its small molecular weight (14 kDa), DENV may also enter diffusion. While some drugs inhibit few known to block...
Finding an effective therapy against diseases caused by flaviviruses remains a challenge. Here, we present protocol to test Food and Drug Administration-approved drugs that inhibit host nuclear protein import, promoting reduction of dengue infection. We describe steps for analyzing the drug effect on import inhibition cellular viral proteins confocal microscopy or western blotting. then procedures measuring antiviral effects virus-infected cells flow cytometry testing efficacy in...