A5076143055- Genomics and Phylogenetic Studies
- Marine Sponges and Natural Products
- Microbial Fuel Cells and Bioremediation
- Microbial Community Ecology and Physiology
- Microbial Natural Products and Biosynthesis
- Environmental DNA in Biodiversity Studies
- Electrochemical Analysis and Applications
- Protist diversity and phylogeny
- Enzyme Production and Characterization
- Wastewater Treatment and Nitrogen Removal
- Electrochemical sensors and biosensors
- Plant-Microbe Interactions and Immunity
- Membrane Separation Technologies
- Microbial Metabolic Engineering and Bioproduction
- Anaerobic Digestion and Biogas Production
- Seaweed-derived Bioactive Compounds
- Chromium effects and bioremediation
- Biopolymer Synthesis and Applications
- Bacterial Genetics and Biotechnology
- Plant Pathogenic Bacteria Studies
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Plant tissue culture and regeneration
- Water Treatment and Disinfection
- Analytical Chemistry and Sensors
Singapore Centre for Environmental Life Sciences Engineering
2016-2023
Nanyang Technological University
2015-2023
National University of Singapore
2010-2019
UNSW Sydney
2005-2011
J. Craig Venter Institute
2009
Technical University of Denmark
2005
University of California, Santa Cruz
2005
Persistence of the opportunistic bacterial pathogen Vibrio cholerae in aquatic environments is principal cause for seasonal occurrence cholera epidemics. This causality has been explained by postulating that V. forms biofilms association with animate and inanimate surfaces. Alternatively, it proposed pathogens are an integral part natural microbial food web thus their survival constrained protozoan predation. Here, we report both explanations interrelated. Our data show protective agent...
Abstract Volatile organic compounds (VOCs) are commonly used as solvents in various industrial settings. Many of them present a challenge to receiving environments, due their toxicity and low bioavailability for degradation. Microorganisms capable sensing responding surroundings this makes ideal detectors toxic compounds. This study investigates the global transcriptomic responses Escherichia coli K-12 selected VOCs at sub-toxic levels. Cells grown presence were harvested during exponential...
The functional metagenomic screening of the microbial communities associated with a temperate marine sponge and green alga identified three novel hydrolytic enzymes antibacterial activities. results suggest that uncultured alpha- gammaproteobacteria contain new classes proteins may be source agents.
A new cyclic decapeptide, trikoramide (1), has been isolated from samples of the marine cyanobacterium Symploca hydnoides, collected Bintan Island, Indonesia. Trikoramide (1) is a C-prenylated cyclotryptophan-containing cyanobactin. Its planar structure was deduced by 1D and 2D NMR spectroscopy as well HR–MS/MS data. In addition, its absolute configuration determined Marfey's method NOESY spectroscopic analysis. Compound 1 possessed cytotoxicity against MOLT-4 AML2 cancer cell lines with...
Abstract Methods for the study of member species in complex microbial communities remain a high priority, particularly rare and/or novel that might play an important ecological role. Specifically, methods link genomic information with its spatial structure are lacking. This adopts integrative workflow permits characterisation previously unclassified bacterial taxa from microbiomes through: (1) imaging structure; (2) taxonomic classification and (3) genome recovery. Our attempts to bridge...
Metagenomics provides access to the uncultured majority of microbial world. The approaches employed in this field have, however, had limited success linking functional genes taxonomic or phylogenetic origin organism they belong to. Here we present an efficient strategy recover environmental DNA fragments that contain marker from metagenomic libraries. Our method involves cleavage 23S ribsosmal RNA (rRNA) within pooled library clones by homing endonuclease I-CeuI followed insertion and...
Collagen is an important, extracellular structural protein for metazoans and provides a rich nutrient source bacteria that possess collagen-degrading enzymes. In symbiotic host system, collagen degradation could benefit the bacteria, but would be harmful eukaryotic host. Using polyphasic approach, we investigated presence of collagenolytic activity in bacterial community hosted by marine sponge Cymbastela concentrica. Functional screening collagenase using metagenomic library clones (227Mbp)...
Electrochemically-active microorganisms couple the degradation of organic matter to production electricity via extracellular electron transfer. They are usually enriched from sediments growth at certain potentials, either in microbial fuel cells or potentiostat-controlled electrochemical cells. Despite great diversity natural and man-made environments, most enrichments select a small group dissimilatory metal-reducing microorganisms. In this study, we aim enrich novel...