Abstract Most rivers of the world are supersaturated with carbon dioxide (CO 2 ), resulting in a large gas flux that has only recently been included global budgeting. However, little is known about CO emissions from urban river networks suffering anthropogenic disturbance context urbanization. We surveyed partial pressure ( p ) and 84 locations Chongqing metropolitan network, an area 5494 km . The overall mean fluxes were 2152 µatm 163.0 mol C m −2 yr −1 , respectively, 318.9 completely 49.6...

River flow alterations caused by dams have introduced many ecological problems, in particular a decline aquatic species such as fishes. One compensatory measure is to create hydrological process similar the natural state with regard survival requirements of fish. In recent years, Three Gorges Reservoir (TGR) has man-made flood operation experiments facilitate spawning four major Chinese carps Yangtze River, China. To investigate fish activities and their responses TGR operation, eggs from...

Boletus bainiugan has a unique flavor profile, its quality is correlated with metabolites. Herein, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) utilized to characterize the free amino acid and organic of at different drying temperatures. Attenuated total internal reflectance Fourier transform infrared (ATR-FTIR) spectroscopy employed identify various treatment predicted compounds. The metabolome includes 72 acids 64 acids, wherein, 11 important taste...

Expressed sequence tags (ESTs) provide a valuable resource for the development of simple repeat (SSR) or microsatellite markers. This study identified SSRs within ESTs from Nelumbo nucifera (lotus sacred lotus), developed markers them, and assessed potential those diversity analysis. Within 2207 N. downloaded GenBank, 1483 unigenes (303 contigs 1180 singletons) were identified. After eliminating redundancy, 125 SSR-containing derived, 71 unique detected with an average density one SSR per...

China’s largest freshwater lake, Poyang Lake, is characterized by rapid changes in its inundation area and hydrodynamics, so this study, a hydrodynamic model of Lake was established to simulate these long-term changes. Inundation information extracted from Moderate Resolution Imaging Spectroradiometer (MODIS) remote sensing data used calibrate the wetting drying parameter assessing accuracy simulated boundary. The bottom friction calibrated using current velocity measurements Acoustic...

Boletes are recognized as a worldwide delicacy. Adulteration of the expired and low-value sliced boletes is pressing problem in supply chain commercial boletes. This study aimed at developing rapid method to identify storage duration species boletes, using near-infrared (NIR) spectroscopy. In study, 1376 fruiting bodies wild-grown were collected from 2017 2020 Yunnan, containing four common edible A NIR spectroscopy-based strategy was proposed, that is, ensure they within shelf life firstly;...

Identifying the geographical origin of Gastrodia elata Blume contributes to scientific and rational utilization medicinal materials. In this study, infrared spectroscopy was combined with machine learning algorithms distinguish G. BI.

ABSTRACT Gastrodia elata Blume from different regions varies in growth conditions, soil types, and climate, which directly affects the content quality of its medicinal components. Accurately identifying origin can effectively ensure value G. Bl., prevent circulation counterfeit products, thus protect interests health consumers. Attenuated total reflectance Fourier transform infrared (ATR‐FTIR) spectroscopy is a rapid effective method for verifying authenticity traditional Chinese medicines....

Abstract The sacred lotus ( Nelumbo nucifera Gaertn.) is an aquatic plant of economic and ornamental importance in China. From (AG) n ‐enriched genomic library, 24 microsatellites were isolated identified by using the (fast isolation AFLP sequences containing repeats) FIASCO protocol. Eleven loci showed polymorphism with two to six alleles per locus. These markers yielded 42 a survey 32 accessions lotus. effective primer pairs simple sequence repeats designed will be used as genetic evaluate...

Wetland reconstruction is an effective way to solve the severe environmental problems caused by natural disasters and human activities. In this study, we implemented three wetland projects based on ecological engineering approaches, woodland dike-pond complex (WDPC), multifunctional multistrip buffer (MMBC), multidimensional (MWC), in water-level fluctuation zone of Hanfeng Lake Three Gorges Reservoir (TGR), China. Then, were evaluated for quantity quality using emergy eco-exergy methods....

Abstract Yunnan is known for its rich biodiversity and as the Wild Mushroom Kingdom. Boletes are a world‐renowned wild edible mushroom, with unique sensory characteristics, nutritional value medicinal extraordinary. However, species geographical origin of boletes influence their price quality. In this study, method was developed to identify simultaneously. Therefore, Fourier transform near‐infrared (FT‐NIR) data sets were collected converted two‐dimensional correlation spectroscopy (2D‐COS)....

Sporotrichosis is a chronic granulomatous infection of the skin and subcutaneous tissue that can affect any organ through lymphatic spread. The prevalence sporotrichosis infections increasing its treatment challenging as there are no unified standard diagnostic techniques or antifungal medications. Controlling further spread requires rapid diagnosis. Assessment clinical symptoms, histological analysis, serological testing, pathogen culture all necessary for diagnosis sporotrichosis. However,...

Temporary freshwater rock pools, as special, small-sized and isolated habitats, provide ideal systems for studying island biogeography ecological theories processes. In this study, a total of 70 potholes mountain rivers were sampled during the four seasons from November 2013 to October 2014 assess structure benthic invertebrate community identify relationships between habitat characteristics composition. Pothole area ranged approximately 0.01 0.39 m2, pothole depth 0.03 0.74 m. Forty-three...

Global gene expression profiling (GGEP) plays a pivotal role in biological research. We developed an improved GGEP method called "robust ordered mRNA differential display (RoDD)" by combining (DD) and complementary DNA amplified fragment length polymorphisms (cDNA-AFLP) using elaborately designed primers poly (dT:A) replacement technique. Redundancy was minimized bead-based isolation coverage restriction enzymes that recognized 4-bp sites. This offers the common virtues of gel-based methods...

Abstract Reservoir impoundment affects fish habitat condition, in turn likely influencing the spatial and temporal distribution patterns of assemblage structure impounded area. Fishing activity instream gravel sand mining are important anthropogenic factors, flow discharge, water temperature, precipitation natural environmental variables; both factors have impacted unimpounded waterbodies. We used multivariate analysis to evaluate combined effects two adjacent reservoir impoundments main...