We previously identified microbial shifts prior to the onset of motor and neurological symptoms within a mouse model fatal neurodegenerative disease Machado-Joseph (MJD). Here, we aimed explore possible mechanisms contributing these changes microbiome-gut-brain axis, whether it preceded or followed central neurodegeneration. report that pre-symptomatic male MJD mice present with significantly different microbiome communities as early 5-weeks-old. Furthermore, show have faster total gut...

To date it’s proved that the elevated homocysteine levels in blood is an independent risk factor for a number of diseases. The animal model rare severe form hyperhomocysteinemia may be necessary to study aspects participation damage organs and tissues various diseases because effects this pathogenetic will most pronounced such conditions. This article describes technique modeling male Wistar rats by three-week intragastric administration methionine suspension with addition amino acid drinking water.

INTRODUCTION: A novel coronavirus (severe acute respiratory syndrome-related 2 (SARS-CoV-2)) emerged in December 2019 and rapidly spread over the world having provoked a pandemic of disease. This highly pathogenic virus can attack lung tissue derange gas exchange leading to distress syndrome systemic hypoxia. Hypoxic conditions trigger activation adaptation mechanisms including hypoxia-inducible factor-1á (HIF-1á) which is involved regulation key processes, e. g, proliferation metabolism...

Background: The aim of this work is to study the significance carnitine fractions and NO metabolites for assessment spermatozoa resistance cryopreservation, which purpose these parameters were investigated in seminal plasma animals divided according indices stallions subjected cryopreservation. Methods: There 18 breeding with an average age 11.28±5.46 years, all Arabian breed. Using cluster analysis indicators semen quality, identified into two groups: high low sperm...

Introduction. Recent work demonstrates that HIF-1α and NOS isoforms are detectable in plasma, representing their potential practical relevance as biomarkers. At the same time, data on stability of these proteins plasma contradictory. Aim study. To investigate amount iNOS patients with COVID-19 infection different severity to assess possi-bilities adaptation hypoxia. Material methods. The pilot study involved 50 divided into three groups: 1) 17 moderate disease; 2) pa-tients severe condition;...

Introduction. Currently, osteoarthritis (OA) has been identified as a polyetiological disease, where several phenotypes have based on the leading frequency. Obviously, each clinical phenotype corresponds only to its fundamental changes part of various organs and systems. Un-til recently, special attention was paid study metabolic processes in state cartilage, but more interest focused biochemistry subchondral bone, microarchitectonics signal function. Aid. To features manifestation...

Background: The aging process causes changes in the structure and functioning of body systems, including immune system. aim this study was to assess oxidative status peripheral blood mononuclear cells (PBMCs) young (3-5 years old), full-aged (6-15 old) older stallions (16-25 old). Methods: level protein carbonyl derivatives, low medium molecular weight substances, activity superoxide dismutase, catalase, g-glutamyl transpeptidase, acid phosphatase, lactate dehydrogenase were studied PBMCs 31...

Рязанский государственный медицинский университет имени

INTRODUCTION: The study of markers oxidative stress, metabolites nitric oxide (II) (NOx) and the balance carnitine fractions in mitochondria rat epididymis, permits to evaluate protective role L-arginine experimental hyperhomocysteinemia.
 AIM: To influence on parameters energy metabolism, level NO metabolites, modification proteins head tail epididymis MATERIALS AND METHODS: In animals group 1 (n = 8), severe hyperhomocysteinemia (HHcy) was modeled by administration methionine...

Aim. To study the concentration of succinate and activity dehydrogenase (SDH) mononuclear blood leukocytes as markers rapid adaptation mitochondria to hypoxia in patients with exacerbation chronic obstructive pulmonary disease (COPD).
 Materials Methods. The involved 58 COPD 13 conventionally healthy volunteers 40-75 years age. In accordance GOLD 2018 principles complex assessment, were divided groups B (n=18), C (n=20), D (n=20) comparable age, FEV1 pack-of-cigarettes/year index....

Hyperhomocysteinemia is a risk factor for many human diseases, including pulmonary pathologies. In this context much interest attracts secondary mitochondrial dysfunction, which an important link in pathogenesis of diseases associated with hyperhomocysteinemia. The study was conducted using male Wistar rats. It found that under conditions severe hyperhomocysteinemia caused by administration methionine, homocysteine accumulated lung mitochondria thus suggesting direct toxic effect on these...

Aim. To study the levels of nitric oxide (II) metabolites, lactate, endogenic carnitine and mitochondrial oxidoreductase activity in epididymis tissue at low synthesis and, based on obtained data, to assess functional condition rat mitochondria. Methods. 16 Wistar rats were allocated two equal groups: The first group was administered L-NG-nitroarginine methyl ester (L-NAME), which is non-selective NO-synthase inhibitor, a dose 25 mg/kg for 7 days. second (the control group) got 0.9% sodium...

Background: Stallion sperms are very sensitive to cryopreservation. Free radicals generated during cryopreservation lead spermatozoa membrane damage, decline in their viability and change the activity of cysteine cathepsins. On other hand, cathepsins may involve utilization oxidatively modified proteins. The present study was conducted assess level proteins seminal plasma stallions with a low ( less than 30%) normal greater percentage live post Methods: A total 33 breeding comprising 6...

Introduction. Melatonin, by inducing the expression of antioxidant enzymes and signaling proteins, enhances effects in development pathological processes occurring against background oxidative stress, therefore stabilization protective principles melatonin is a rel-evant area research, allowing us to identify new ways treatment osteoarthritis. The aim. To study effect on succinate receptor system chondrocytes osteoarthritis under conditions stress vitro. Materials methods. vitro was carried...

Aim. To study the indicators of energy metabolism and oxidative stress in mononuclear leukocytes peripheral blood to assess possibility mitochondrial dysfunction development chronic obstructive pulmonary disease bronchitis.
 Methods. The included 50 patients aged 40 75 years with (COPD) or bronchitis. first group 13 In accordance GOLD spirometric classification, second third groups COPD moderate severity (COPD 2) (n=17) severe 3) (n=20) respectively. isolated leukocytes, activity...

BACKGROUND: Hypoxia is a factor of development many diseases, and, among other things, may be the cause male infertility. The molecular basis pathogenesis in hypoxia can serve for methods treatment and correction. AIM: model chronic normobaric laboratory animals. MATERIALS AND METHODS: study involved 32 mature rats Wistar line 200–280 g mass. animals were divided to 2 experimental groups their control groups. In first model, acute with hypercapnia was used one time according method M. V....

Abstract The aim of this work was to study the relationship between acute phase indicators a biochemical blood test with quality and cryostability sperm in stallions. Semen from 59 stallions (aged 5 15 years) were collected during breeding season (February–May). Blood plasma samples studied using Vegasys automatic analyzer (Analyzer Medical System, Italy). following parameters determined plasma: total protein, level albumin, globulin, albumin/globulin ratio, C-reactive fibrinogen. Each...

Introduction Osteoarthritis (OA) has long been considered from the mechanical point of view etiology and pathogenesis, as a "wear tear" disease. However, advances in fundamental medicine have changed way we look at this The role systemic inflammation was determined OA progression risk factor related clinical phenotypes were differentiated post-traumatic OA, age associated genetic metabolic syndrome OA. development disorders envision concept regulation osteocartilaginous synovial tissues,...

Background: Artificial insemination with chilled sperm is a reproductive biotechnology often used in horse breeding. However, maintaining the acceptable quality after cooling, which affects frequency of pregnancy, remains an urgent problem at present time. Therefore, clarifying role important physiological metabolites, such as L-carnitine and nitric oxide, significant aspect terms possible improvement cryopreserved sperm. Methods: The study included 35 breeding stallions, 27 were stallions...

Background. The molecular mechanism of NO effects in adaptation to hypoxia is interest as a potential point application the therapy fertility disorders. Aim. To assess degree oxidative modification epididymal mitochondrial proteins during under conditions experimentally modified synthesis. Material and methods. Sexually mature rats were divided into four groups 8 individuals: (1) control (animals without hypoxia); (2) chronic normobaric hypoxia, animals kept sealed chamber with oxygen...