The lipid composition of the sperm membrane has a significant effect upon functional characteristics spermatozoa. In present study we investigated fatty acid (FA) subpopulations spermatozoa separated on discontinuous Percoll gradient (47:90%) and FA phospholipids (PL) heads tails in both normal abnormal semen samples. normozoospermic samples, polyunsaturated acids (PUFA) represented 34.0 +/- 1.3 (mean SE, mole %) 25.6 1.2% total PL 47 90% fractions respectively. Docosahexaenoic (22:6omega3,...

Journal Article Evaluation of the role reactive oxygen species in male infertility Get access Adel Zalata, Zalata Search for other works by this author on: Oxford Academic PubMed Google Scholar Tarek Hafez, Hafez Frank Comhaire 1 1To whom correspondence should be addressed Human Reproduction, Volume 10, Issue 6, June 1995, Pages 1444–1451, https://doi.org/10.1093/HUMREP/10.6.1444 Published: 01 1995 history Received: 12 September 1994 Accepted: 02 March

Use of cellular phones emitting radiofrequency electromagnetic field (RF-EMF) has been increased exponentially and become a part everyday life. This study aimed to investigate the effects in vitro RF-EMF exposure emitted from on sperm motility index, DNA fragmentation seminal clusterin (CLU) gene expression. In this prospective study, total 124 semen samples were grouped into following main categories: i. normozoospermia (N, n=26), ii. asthenozoospermia (A, n=32), iii....

ABSTRACT: The presence of various cytokines, namely hepatocyte growth factor (HGF), interleukin‐1 receptor antagonist (IL‐1 RA), and interleukins (IL‐1α, IL‐6, IL‐8), as well the production reactive oxygen species (ROS) was investigated in seminal plasma fertile infertile patients order to evaluate possible value measuring these substances for diagnosis male accessory gland infection, assess relationship between oxidative stress cytokines during leucocytospermia infection (MAGI). Our...

Measurement of alpha-glucosidase (alpha-GLUC) activity by means a simple colorimetric test using commercial kit (EpiScreen; FertiPro, Lotenhulle, Belgium) yielded results that were strongly correlated with the values obtained for neutral iso-enzyme measured fluorimetric reference method (r=0.85, P=0.003, n=13). The former was characterized low intra- and inter-coefficient variation (6.6 4.3% respectively). Vasectomized men azoospermia (n=27) had significantly lower alpha-GLUC in semen than...

The presence of 2 million or more peroxidase-positive white blood cells per ml semen, the diagnosis male accessory gland infection, is associated with important biochemical and biological changes in semen plasma spermatozoa, reducing their fertilizing potential vitro vivo (e.g., during intra-uterine insemination). In addition to effects reactive oxygen species, its influence on essential fatty acid composition sperm membrane, potentially unfavourable can occur through intermediate increased...

CLU is a disulfide linked, heterodimeric protein associated with the clearance of cellular debris and apoptosis. We assessed association seminal gene expression variables in fertile infertile men.A total 124 men were divided into healthy, normozoospermia, asthenozoospermia, asthenoteratozoospermia oligoasthenoteratozoospermia. History was obtained, clinical examination semen analysis done. In we sperm acrosin activity, DNA fragmentation expression.CLU RNA significantly increased samples...

The lipid composition of the sperm membrane has been shown to exert a significant effect upon functional quality spermatozoa. We have studied induced peroxidation and presence polymorphonuclear white blood cells (WBCs) on fatty acid phospholipids human spermatozoa were fractionated by discontinuous Percoll gradient in two fractions (47% 90% Percoll). Induced was assessed determining production thiobarbituric reactive substances (TBARS), mostly malondialdehyde, after incubation with ferrous...

Follicle-stimulating hormone (FSH) is fundamental for Sertoli cell function stimulating spermatogenesis and follicular growth by a specific receptor (FSHR). This work aimed to investigate the occurrence of Asn Ser FSHR gene variants its relationship with seminal anti-Müllerian (AMH) among normozoospermic infertile oligoasthenozoospermic (OAT) males. Eighty-two Caucasian males grouped into healthy controls (n = 30) OAT 52). were determined DNA from anti-coagulated blood underwent polymerase...

No AccessJournal of UrologyAdult Urology1 Jun 2013Androgen Receptor Expression Relationship with Semen Variables in Infertile Men Varicocele Adel A. Zalata, Naglaa Mokhtar, Abd El-Naser Badawy, Gamal Othman, Moheiddin Alghobary, and Taymour Mostafa ZalataAdel Zalata Medical Biochemistry Department, Faculty Medicine, Mansoura University, Mansoura, Egypt , MokhtarNaglaa Mokhtar BadawyAbd Badawy OthmanGamal Othman AlghobaryMoheiddin Alghobary Dermatology, Venereology Andrology MostafaTaymour...

Approximately 15% of couples are affected by infertility with the man responsible in almost half cases. PRMs (protamines) confer a higher order DNA packaging sperm than that somatic cells. Because critical roles spermatid differentiation, aberrations PRM expression or changes protein structure could be causes certain types idiopathic human male infertility. The aim this study was to give insight into role PRM2 gene and caspase 9 activity pathogenesis infertility.The current included 70 men...

Human semen contains large amounts of opioid peptides and cytokines. We have measured the concentrations interleukin (IL)-6 in 140 samples β-endorphinin 77 samples. The median concentration βendorphinin seminal plasma from normozoospermic men(n = 23) was 154.7 pg/ml (10th—90th percentiles, 42.0—774.6), there no significant difference β-endorphin among normozoospermic, oligozoospermic (n= 28), asthenozoospermic 15), azoospermic(n= 4) post-vasectomy 7) There correlation between sperm...

Cypermethrin, a type II synthetic pyrethroid pesticide, is widely used in pest control programmes agriculture and public health. This study aimed to assess the potential effect of cypermethrin on human spermatozoa possible ameliorative effects vitamins C E. Semen samples 20 healthy normozoospermic men were divided into six aliquots at room temperature. The first aliquot served as not exposed treatments, second was incubated with mm vit. 2 E where third one 10 μm for 6 h. other three C, both...

Summary Androgens in seminal plasma have been investigated relation to other markers of the male reproductive system. Testosterone and dihydrotestosterone (DHT) were measured by radioimmunoassay after paper chromatography 104 semen samples. Concentrations testosterone DHT significantly lower samples from patients with abnormal sperm characteristics than men normozoospermia. Both correlated concentration ( r = 0.40, p < 0.001; 0.41, 0.001, respectively), motile 0.35, 0.01; 0.01) ATP 0.59,...

Sperm lipids are important for sperm viability, maturity and function. This study aimed to identify cholesterol desmosterol composition of human spermatozoa two populations separated on Sil-Select gradient. Forty-eight males were divided into four groups namely healthy men (n = 13), asthenozoospermia 11), asthenoteratozoospermia 10) oligoasthenoteratozoospermia 14). estimated in population by centrifugation a discontinuous The results showed that the major sterols spermatozoa. Spermatozoa...

The aim of this study was to assess the long-term toxic effect ofloxacin on testes and epididymides 72 adult male albino rats. rats were divided into group A B. Group A, which received for 14 days, subdivided two subgroups; LD-14 low dose mg KBW(-1) daily HD-14 high 216 daily. B, 28 LD-28 HD-28 Two matched control groups followed up days respectively. animals evaluated body weight, testicular relative serum testosterone (T), epididymal sperm analysis (sperm count, motility, morphology,...

No AccessJournal of UrologyAdult Urology1 May 2012ACE Gene Insertion/Deletion Polymorphism Seminal Associations in Infertile Men Adel A. Zalata, Heba K. Morsy, Abd El-Naser Badawy, Samir Elhanbly, and Taymour Mostafa ZalataAdel Zalata Department Medical Biochemistry, Faculty Medicine, Mansoura University, Mansoura, Egypt , MorsyHeba Morsy BadawyAbd Badawy ElhanblySamir Elhanbly Dermatology Andrology, MostafaTaymour Andrology Sexology, Cairo Cairo, View All Author...

Because of its distinctive ability to act as a mitogen, mitogen and morphogen, hepatocyte growth factor/ scatter factor (HGF/SF) has all the characteristics molecule able function in regulatory networks motility, such spermatogenic epithelium, this through binding receptor p190MET (C-MET). In study we report expression C-MET human seminiferous epithelium on spermatozoa from men being treated for infertility sperm donors. The presence was demonstrated by immunochemistry cell membrane...

The production of reactive oxygen species (ROS) from spermatozoa has been measured by chemiluminescence in the two fractions a Percoll gradient column (47 and 90%). Chemiluminescent signals were recorded each fraction after addition luminol horse-radish peroxidase (basal state), stimulation with formylmethionyl-leucyl-phenyl-alanine (FMLP) phorbol ester (PMA). We found an inverse correlation between reducing capacity semen as estimated resazurin test, ROS spermatozoa, especially PMA (r =...