ABSTRACT Lactococcus garvieae (junior synonym Enterococcus seriolicida ) is an emerging zoonotic agent isolated from economically important fish (rainbow trout and yellowtail), cattle, humans. Clindamycin susceptibility the only phenotypic test which can differentiate L. lactis , another in A PCR assay for identification of was developed resulted amplified fragment 1,100 bp size. The shown to be specific . positive all strains tested, originated three different continents (Asia, Australia,...

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 48:101-108 (2002) - doi:10.3354/dao048101 Initial characteristics koi herpesvirus and development a polymerase chain reaction assay detect virus in koi, Cyprinus carpio Oren Gilad1, Susan Yun1, Karl B. Andree1, Mark A. Adkison1, Amir Zlotkin2, Herve Bercovier2, Avi Eldar3, Ronald P. Hedrick1,* 1Department Medicine...

The recent emergence of carbapenem resistance among Enterobacteriaceae is a major threat for hospitalized patients, and effective strategies are needed.To assess the effect an intensified intervention, which included active surveillance, on incidence infection with carbapenem-resistant Klebsiella pneumoniae.Sheba Medical Center, 1,600-bed tertiary care teaching hospital in Tel Hashomer, Israel.Quasi-experimental study.The medical records all patients who acquired K. pneumoniae during 2006...

Abstract Background Outbreaks with mass mortality among common carp Cyprinus carpio and koi have occurred worldwide since 1998. The herpes-like virus isolated from diseased fish is different Herpesvirus cyprini channel catfish was accordingly designated herpesvirus (KHV). Diagnosis of KHV infection based on viral isolation current PCR assays has a limited sensitivity therefore new tools for the diagnosis infections are necessary. Results A robust sensitive assay defined gene sequence...

ABSTRACT Streptococcus iniae was isolated from diseased wild fish collected near a mariculture facility where gilthead sea bream and European bass exhibited similar infection. Species-specific PCR ribotyping confirmed that cultured were infected by single S. clone. Wild are therefore potential amplifiers of pathogenic strains.

To detect bacteriophages for Gram-positive oral pathogens in human saliva.Saliva samples from 31 donors were screened the presence of Streptococcus sobrinus, mutans, salivarius, Actinomyces viscosus and Enterococcus faecalis. Bacteriophages faecalis found seven samples. phages still present saliva re-collected one donor month, year after initial collection.The stability suggests a possible role these ecosystem.Phage therapy as way to control bacteria might be considered.

ABSTRACT Lactococcus garvieae (junior synonym, Enterococcus seriolicida ) is a major pathogen of fish, producing fatal septicemia among fish species living in very diverse environments. The phenotypic traits L. strains collected from three different continents (Asia, Europe, and Australia) indicated heterogeneity. On the basis acidification d -tagatose sucrose, biotypes were defined. DNA relatedness values specific PCR assay showed that all belonged to same genospecies, . All serotyped as...

ABSTRACT Streptococcus iniae was recovered from diseased rainbow trout ( Oncorhynchus mykiss , Walbaum) previously vaccinated against streptococcosis. PCR and serological methods indicate the presence of a new serotype in fish.

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 49:165-170 (2002) - doi:10.3354/dao049165 Streptococcus iniae infections in Red Sea cage-cultured and wild fishes A. Colorni1,*, Diamant1, Eldar2, H. Kvitt1, Zlotkin3 1Israel Oceanographic Limnological Research, National Center for Mariculture, PO Box 1212, Eilat 88112, Israel 2Department Poultry Fish Diseases, The...

The salmonid macrophage-like cell line RTS-11 and purified trout pronephros phagocytes were used to analyze in vitro entry survival of two Streptococcus iniae serotypes. Efficient invasion by S. occurred both cells, but only the type II strain persisted for at least 48 h. Ex vivo models opsonin-dependent phagocytosis demonstrated increased efficacy. Analysis collected from diseased fish that approximately 70% bacteria contained blood during septic phase disease located within phagocytes,...

ABSTRACT A molecular characterization of two Mycobacterium marinum genes, 16S rRNA and hsp65 , was carried out with a total 21 isolates from various species fish both marine freshwater environments Israel, Europe, the Far East. The nucleotide sequences genes revealed that all M . in Israel belonged to different strains, one infecting (cultured wild) other (cultured) fish. restriction enzyme map based on confirmed divergence Israeli differentiated foreign isolates, exception three Greek which...

ABSTRACT Infection with Lactococcus garvieae is considered the most important risk factor for European trout industry, and losses are approximately 50% of total production. To improve our understanding genetic links among strains originating from different countries, we examined population structure L. by comparing 81 isolated sources ecosystems (41 farms in six countries) which bacterium commonly found. Genetic similarities (as assessed molecular tools, including restriction fragment length...

ABSTRACT Streptococcus iniae is a major pathogen of fish, producing fatal disease among fish species living in very diverse environments. Recently, reoccurrences outbreaks were recorded rainbow trout ( Oncorhynchus mykiss , Walbaum) farms where the entire population was routinely vaccinated. New strains are distinguished from previous by their ability to produce large amounts extracellular polysaccharide that released into medium. Present findings indicate antigenic factor, suggesting an...

Surgical wound infections caused by rapidly growing mycobacteria developed in 15 women after insertion of breast implants from August to November 2003 at a single medical center.A case-control study was conducted that included the identified patients, as well who underwent operations same center did not develop infections. The accompanied an extensive environmental investigation. Isolates were standard bacteriological methods and comparison their 16S rRNA, HSP65, RPOB, SODA, RECA gene...

Creation of surfaces resistant to the formation microbial biofilms via biomimicry has been heralded as a promising strategy protect range different materials ranging from boat hulls medical devices and surgical instruments. In our current study, we describe successful transfer highly effective natural marine biofilm inhibitor 2D surface format. A series cyclic peptides inspired by equinatoxin II protein produced Beadlet anemone (Actinia equine) have evaluated for their ability inhibit mixed...

Streptococcus iniae is a major pathogen of fish, causing considerable economic losses in Israel, the United States and Far East. Containment mortalities through vaccination was recently compromised due to emergence novel vaccine-escape strains that are distinguished from previous by their ability produce large amounts extracellular polysaccharide (EPS) released medium. In vitro vivo data now indicate EPS virulence factor, capable triggering proinflammatory cytokine machinery inducing...

Heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) bacteremia is an emerging infection. Our objective was to determine the molecular features of hVISA strains isolated from bacteremic patients and compare them methicillin resistant S. (MRSA) sensitive (MSSA) blood isolates. We assessed phenotypic genomic changes (n = 24), MRSA 16) MSSA 17) isolates by PCR staphylococcal chromosomal cassette (SCCmec) types, Panton-Valentine leukocidin (PVL) accessory gene regulator (agr)...

Abstract Microbiologically influenced corrosion (MIC) is caused by problematic biofilms in many industries, especially the oil and gas industry. In this work, a novel peptide (labeled as “Peptide A”) was used to enhance tetrakis hydroxymethyl phosphonium sulfate (THPS) biocide treat corrosive reducing bacterium (SRB) Desulfovibrio vulgaris tough field biofilm consortium “Consortium II”) on C1018 carbon steel coupons. This synthesized based active sequence derived from an anti-biofilm protein...