A5053510806- Antimicrobial Peptides and Activities
- Marine Sponges and Natural Products
- Marine Biology and Environmental Chemistry
- Analytical chemistry methods development
- Marine Ecology and Invasive Species
- Analytical Chemistry and Chromatography
- Chemical Synthesis and Analysis
- Biochemical and Structural Characterization
- Antimicrobial agents and applications
- Cholinesterase and Neurodegenerative Diseases
- Environmental Toxicology and Ecotoxicology
- Mass Spectrometry Techniques and Applications
- Chemical synthesis and alkaloids
- Protein Interaction Studies and Fluorescence Analysis
- Microbial Natural Products and Biosynthesis
- Microfluidic and Capillary Electrophoresis Applications
- Bioactive Compounds and Antitumor Agents
- Computational Drug Discovery Methods
- Protein purification and stability
- Molecular spectroscopy and chirality
- Protein Hydrolysis and Bioactive Peptides
- Forensic Toxicology and Drug Analysis
- Synthesis and Biological Activity
- Microplastics and Plastic Pollution
- Seaweed-derived Bioactive Compounds
Cawthron Institute
2020-2025
RISE Research Institutes of Sweden
2017-2023
UiT The Arctic University of Norway
2010-2020
University of Gothenburg
2020
Linnaeus University
2019
University of Borås
2015-2019
Bioscience (China)
2018
Centre for Arctic Gas Hydrate, Environment and Climate
2016
Tromsø research foundation
2016
University of Coimbra
2010
The use of non-standard toxicity models is a hurdle in the early development antimicrobial peptides towards clinical applications. Herein we report an extensive vitro and vivo study library 24 peptide-based antimicrobials with narrow spectrum activity veterinary pathogens. haemolytic compounds was evaluated against four different species relative sensitivity highest for canine erythrocytes, intermediate rat human cells lowest bovine cells. Selected were additionally HeLa, HaCaT HepG2 which...
The agricultural production of ruminants is responsible for 24% global methane emissions, contributing 39% emissions this greenhouse gas from the sector. Strategies to mitigate ruminant methanogenesis include use methanogen inhibitors. For example, seaweeds Asparagopsis taxiformis and armata included at low levels in feed cattle sheep inhibit by up 98%, with evidence improvements utilisation efficiency. This has resulted an increasing interest demand these globally. In response, research...
Biofouling is a major problem shared among all maritime sectors employing submerged structures where it leads to substantially increased costs and lowered operational lifespans if poorly addressed. Insight into the ongoing processes at relevant marine locations key effective management of biofouling. Of specific concern for renewable energy (MRE) sector fact that information on biofouling composition magnitude across geographies dispersed throughout published papers consulting reports. To...
Abstract Background Many biologically active compounds bind to plasma transport proteins, and this binding can be either advantageous or disadvantageous from a drug design perspective. Human serum albumin (HSA) is one of the most important proteins in cardiovascular system due its great capacity high physiological concentration. HSA has preference for accommodating neutral lipophilic acidic drug-like ligands, but also surprisingly able positively charged peptides. Understanding how short...
ConspectusThe unwanted accumulation of marine micro- and macroorganisms such as algae barnacles on submerged man-made structures vessel hulls is a major challenge for any operation. Known biofouling, this problem leads to reduced hydrodynamic efficiency, significantly increased fuel usage, microbially induced corrosion, and, if not managed appropriately, eventual loss both performance structural integrity. Ship hull biofouling in the international maritime transport network conservatively...
The inherent instability of peptides toward metabolic degradation is an obstacle on the way bringing potential peptide drugs onto market. Truncation can be one to increase proteolytic stability peptides, and in present study susceptibility against trypsin, which major enzymes gastrointestinal tract, was investigated for several short diverse libraries promising cationic antimicrobial tripeptides. Quite surprisingly, trypsin able cleave very small at a substantial rate. Isothermal titration...
The interactions between a range of small cationic antibacterial tripeptides and bovine human serum albumin in buffered aqueous solution at 25 °C have been studied using isothermal titration calorimetry. Results from the binding study indicate single site on with dissociation constant 4.3 22.2 μM for different peptides. In theoretical mouse model, this corresponds to 95% binding. effect interaction capacity peptides against Staphylococcus aureus, strain ATCC 25923 was by including assays...
Bioassay-guided fractionation of the sub-Arctic ascidian Synoicum pulmonaria collected off Norwegian coast led to isolation a novel family brominated guanidinium oxazolidinones named synoxazolidinones A and B (1 2). The backbone compounds contains 4-oxazolidinone ring rarely seen in natural products. structure was determined by spectroscopic methods. exhibited antibacterial antifungal activities.
Turning a new leaf: The first structures isolated from Thuiaria breitfussi, the breitfussins, are presented. This structural class consists of indole–oxazole–pyrrole units. Limited quantities prevented crystallization; therefore, were solved using novel combination AFM, computer-aided structure elucidation (CASE), and DFT calculations. Visualization by AFM determined all connection points cyclic systems other substituents.
The current study describes the antifouling properties of four members belonging to recently discovered synoxazolidinone and pulmonarin families, isolated from sub-Arctic sessile ascidian Synoicum pulmonaria collected off Norwegian coast. Four simplified synthetic analogues were also prepared included in study. Several studied compounds displayed MIC values micro-nanomolar range against 16 relevant marine species involved both micro- macrofouling process. Settlement studies on Balanus...
The current study represents the first comprehensive investigation into general antifouling activities of natural drimane sesquiterpene polygodial. Previous studies have highlighted a high effect toward macrofoulers, such as ascidians, tubeworms, and mussels, but no reports about polygodial been communicated before. To probe structural chemical basis for activity, library 11 analogues was prepared by semisynthesis. designed to yield derivatives with ranging polarities ability engage in both...
ConspectusThe alarming rate at which micro-organisms are developing resistance to conventional antibiotics represents one of the global challenges our time. There is currently ample space in antibacterial drug pipeline, and scientists trying find innovative novel strategies target microbial enemies. Nature has remained a source inspiration for most developed used, immune molecules produced by innate defense systems, as first line defense, have been heralded next antibiotics. Most living...
Chiroptical techniques are increasingly employed for assigning the absolute configuration of chiral molecules through comparison experimental spectra with theoretical predictions. For assignment natural products, electronic chiroptical spectroscopies such as circular dichroism (ECD) routinely applied. However, sensitivity spectral parameters to conditions and methods can lead incorrect assignments. Vibrational (vibrational dichroism, VCD, Raman optical activity, ROA) provide more reliable...
Barettin, 8,9-dihydrobarettin, bromoconicamin and a novel brominated marine indole were isolated from <italic>Geodia barretti</italic> evaluated as inhibitors of electric eel acetylcholinesterase together with synthetic analogs.
Bioactive venoms and toxins are emerging as a promising source of drug leads. Optimized through evolution, these compounds display remarkable selectivity ligand affinity toward range relevant pharmacological targets. The successful development new drugs from is hampered in some areas by the chemical complexity active compounds, which limits possibility using synthesis or recombinant strategies for lead generation. Marine paralytic shellfish produced marine microalgae one such family...
The betalain pigments in ulluco (Ullucus tuberosus), a tuberous crop native to the Andes, have been investigated for first time using LC−DAD−ESI-MS−MS2 analyses. Five red, yellow, and red-spotted accessions introduced into New Zealand as new food plus two red tetraploid lines were investigated. Thirty-two different betalains identified. Both yellow tubers rich betaxanthins, most prominent among 20 identified histidine-betaxanthin, arginine-betaxanthin glutamine-betaxanthin....
The global problem of microbial resistance to antibiotics has resulted in an urgent need develop new antimicrobial agents. Natural peptides are considered promising candidates for drug development. Echinoderms, which rely on innate immunity factors the defence against harmful microorganisms, sources novel peptides. This study aimed isolate and characterise from Edible sea urchin Echinus esculentus. Using bioassay-guided purification cDNA cloning, three were characterised haemocytes urchin;...
Stationary and slow-moving marine organisms regularly employ a natural product chemical defense to prevent being colonized by micro- macroorganisms. While these antifoulants can be structurally diverse, they often display highly conserved chemistries physicochemical properties, suggesting antifouling pharmacophore. In our current report, we investigate the phidianidine A, which displays several properties found in potent antifoulants. Phidianidine A synthetic analogues were screened against...