A5083276568- Virology and Viral Diseases
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Syphilis Diagnosis and Treatment
- interferon and immune responses
- Mosquito-borne diseases and control
- Genetic factors in colorectal cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Microbial infections and disease research
- Male Breast Health Studies
- Toxin Mechanisms and Immunotoxins
- Viral Infections and Vectors
- Cancer Genomics and Diagnostics
- Viral Infections and Immunology Research
- Lung Cancer Treatments and Mutations
- BRCA gene mutations in cancer
- Parvovirus B19 Infection Studies
- Herpesvirus Infections and Treatments
- Rabies epidemiology and control
United States Food and Drug Administration
2023
Center for Biologics Evaluation and Research
2019-2023
Peter MacCallum Cancer Centre
2014-2015
National Institutes of Health
2008
St Thomas' Hospital
1996-1998
King's College London
1998
London Postgraduate Medical and Dental Education
1996
Guy's Hospital
1995
A reverse transcription-nested PCR assay (RT-PCR) was evaluated for diagnosis of congenitally acquired rubella in utero and during infancy. RT-PCR compared with virus isolation retrospective detection placental fetal tissues obtained after termination pregnancy following primary or reinfection. Concordant results were 85% samples; RNA detected by alone four samples, two samples. Samples also prenatal congenital infection; three seven chorionic villus samples one amniotic fluid RT-PCR, while...
Recent discoveries in cancer research have revealed a plethora of clinically actionable mutations that provide therapeutic, prognostic and predictive benefit to patients. The feasibility screening as part the routine clinical care patients remains relatively unexplored demonstration massively parallel sequencing (MPS) tumours general population is required assess its value towards health-care system. Cancer 2015 study large-scale, prospective, multisite cohort newly diagnosed from Victoria,...
A reverse transcription nested PCR (RT-PCR) assay for the detection of rubella virus RNA using primers from E1 open reading frame was established. This found to be sensitive (detecting approximately two synthetic copies and extracted 0.1 50% tissue culture infective dose virus) specific; five wild-type strains four vaccine were detected, no nonspecific amplification 16 other viruses or RNAs seven cell types occurred. Rubella detected in 12 pharyngeal swabs patients with serologically...
E1 gene nucleotide sequences of 63 rubella virus isolates from North America, Europe, and Asia isolated between 1961 1997 were compared phylogenetically. Two genotypes evident: Genotype I contained 60 viruses Japan, genotype II 3 China India. The prior to 1970 grouped into a single diffuse clade, indicating intercontinental circulation, while most post-1975 segregated geographic clades each continent, evolution in response vaccination programs. amino acid differed by no more than 3%; thus,...
Varicella-zoster virus (VZV) immediate-early 63 protein (IE63) is abundantly expressed during both acute infection in vitro and latent human ganglia. Using the yeast two-hybrid system, we found that VZV IE63 interacts with antisilencing function 1 (ASF1). ASF1 a nucleosome assembly factor which member of H3/H4 family histone chaperones. coimmunoprecipitated colocalized transfected cells expressing VZV-infected cells. also lytic latently enteric neurons. exists two isoforms, ASF1a ASF1b,...
Most viruses are known to spontaneously generate defective viral genomes (DVG) due errors during replication. These DVGs subgenomic and contain deletions that render them unable complete a full replication cycle in the absence of co-infecting, non-defective helper virus. DVGs, especially copyback type, frequently observed with paramyxoviruses, have been recognized be important triggers antiviral innate immune response. therefore gained interest for their potential alter attenuation...
Male breast cancer (MBC) is still poorly understood with a large proportion arising in families history of cancer. Genomic studies have focused on germline determinants MBC risk, minimal knowledge somatic changes these cancers. Using TruSeq amplicon panel, this study evaluated 48 familial MBCs (3 BRCA1 mutant, 17 BRCA2 mutant and 28 BRCAX) for hotspot mutations copy number common genes. Twelve missense included nine PIK3CA (seven BRCAX patients), two TP53 (both patients) one PTEN mutation....
ABSTRACT Synovial fluid samples and/or biopsies from 79 patients with various chronic inflammatory joint diseases or traumatic injury were tested for rubella virus (RV) in order to confirm refute results other studies that suggested RV as a cause of disease. Sixty-eight the 72 had antibodies. RNA was detected by reverse transcription-PCR synovial cells two patients. also isolated cell culture one these This patient 42-year-old female common variable immune deficiency and Mycoplasma hominis...
We have determined the nucleotide sequence of region rubella virus genome which encodes amino acids 195–296 E1 glycoprotein (E1-195–296) from a panel 22 viruses obtained Europe, USA and Asia between 1963–1995. E1-195–296 contains neutralizing haemagglutinating determinants, may represent major antigenic domain. The divergence compared to Therien strain ranged 0.65–7.14%. greatest occurred in two Indian origin, was more than twofold greater that previously reported for virus. majority changes...
Mumps virus (MuV) is a neurotropic non-segmented, negative-stranded, enveloped RNA in the Paramyxovirus family. The 15.4 kb genome encodes seven genes, including V/P, which encodes, among other proteins, V protein. MuV protein has been shown to target cellular signal transducer and activator of transcription proteins STAT1 STAT3 for proteasome-mediated degradation. While targeting generally accepted as means limiting innate antiviral responses, consequence less clear. Further, since targets...
We previously reported a novel rhabdovirus produced from the Spodoptera frugiperda Sf9 cell line, designated as Sf-rhabdovirus X+ since it contained unique accessory gene X. The genome sequence was generated using Sanger sequencing and short-read high-throughput (HTS). In this study, we have used long-read HTS technologies, PacBio’s single-molecule real-time Oxford’s Nanopore RNA direct sequencing, to analyze parent line transcriptome virus an clone, respectively. A 3.7 kb duplication...