A5063872887- Immunotherapy and Immune Responses
- Genetic factors in colorectal cancer
- BRCA gene mutations in cancer
- Male Breast Health Studies
- Cancer Genomics and Diagnostics
- Ubiquitin and proteasome pathways
- Advanced Breast Cancer Therapies
- Histone Deacetylase Inhibitors Research
- Cancer Cells and Metastasis
- Protein Degradation and Inhibitors
- Breast Cancer Treatment Studies
- Cancer Immunotherapy and Biomarkers
- Breast Lesions and Carcinomas
- Endometrial and Cervical Cancer Treatments
- RNA Interference and Gene Delivery
- Occupational and environmental lung diseases
- Cancer Research and Treatments
- Cancer and Skin Lesions
- Colorectal Cancer Treatments and Studies
- Microtubule and mitosis dynamics
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- DNA Repair Mechanisms
- Glycosylation and Glycoproteins Research
- PARP inhibition in cancer therapy
Epworth Hospital
2024
Reef Ecologic
2016-2023
Monash Health
2023
Austin Hospital
2009-2022
Services Australia
2017-2022
Olivia Newton-John Cancer Wellness & Research Centre
2016-2022
The University of Melbourne
2011-2017
Peter MacCallum Cancer Centre
2011-2017
Austin Health
2009-2016
The Royal Melbourne Hospital
2006-2015
Abstract The translation of basic research into improved therapies for breast cancer patients requires relevant preclinical models that incorporate spontaneous metastasis. We have completed a functional and molecular characterisation new isogenic C57BL/6 mouse model metastasis, comparing contrasting it with the established BALB/c 4T1 model. Metastatic EO771.LMB tumours were derived from poorly metastatic parental EO771 mammary tumours. Functional differences evaluated using both in vitro...
Overexpression of the prosurvival protein BCL-2 is common in breast cancer. Here we have explored its role as a potential therapeutic target this disease. BCL-2, anti-apoptotic relatives MCL-1 and BCL-XL, proapoptotic BH3-only ligand BIM were found to be coexpressed at relatively high levels substantial proportion heterogeneous tumors, including clinically aggressive basal-like cancers. To determine whether BH3 mimetic ABT-737 that neutralizes BCL-W had efficacy targeting BCL-2–expressing...
The transcription factor Gata-3 is a definitive marker of luminal breast cancers and key regulator mammary morphogenesis. Here we have explored role for in tumor initiation the underlying cellular mechanisms using mouse model "luminal-like" cancer. Loss single allele markedly accelerated progression mice carrying virus promoter-driven polyomavirus middle T antigen (MMTV-PyMT mice), while overexpression curtailed tumorigenesis. Through identification two distinct progenitor cells gland,...
Drugs that target the Renin-Angiotensin System (RAS) have recently come into focus for their potential utility as cancer treatments. The use of Angiotensin Receptor Blockers (ARBs) and Angiotensin-Converting Enzyme (ACE) Inhibitors (ACEIs) to manage hypertension in patients is correlated with improved survival outcomes renal, prostate, breast small cell lung cancer. Previous studies demonstrate Type I (AT1R) linked pathogenesis, unbiased analysis gene-expression identifying significant...
Male breast cancer (MBC) is an uncommon and relatively uncharacterised disease accounting for <1% of all cancers. A significant proportion occurs in families with a history particular those carrying BRCA2 mutations. Here we describe clinicopathological features genomic BRCA1 mutation status large cohort familial MBCs. Cases (n=60) included 3 25 carries, 32 non-BRCA1/2 (BRCAX) carriers strong family histories cancer. The was examined respect to status, parameters including TNM staging, grade,...
RAD21 is a component of the cohesion complex and integral to chromosome segregation error-free DNA repair. functionally important in tumour progression but its role colorectal carcinoma (CRC) unclear. We therefore assessed clinicopathological prognostic significance CRC, as well effect on chemosensitivity. A retrospective observation study examined expression 652 CRCs using tissue microarray approach. Correlation with factors including gender, grade, mucinous subtype, TNM stage,...
Background Cancer-Testis Antigens (CTAs) are immunogenic proteins that poor prognostic markers in non-small cell lung cancer (NSCLC). We investigated expression of CTAs NSCLC and their association with response to chemotherapy, genetic mutations survival. Methods studied 199 patients pathological N2 treated neoadjuvant chemotherapy (NAC; n = 94), post-operative observation (n 49), adjuvant 47) or unknown 9). Immunohistochemistry for NY-ESO-1, MAGE-A MAGE-C1 was performed. Clinicopathological...
Loss of heterozygosity (LOH) the adenomatous polyposis coli (APC) gene triggers a series molecular events leading to intestinal adenomagenesis. Haploinsufficiency cohesin Rad21 influences multiple initiating in colorectal cancer (CRC). We identify as gatekeeper LOH and β-catenin target provide evidence that Wnt pathway activation drives RAD21 expression human CRC. Genome-wide analyses identified key transcriptional regulator critical CRC genes long interspersed element (LINE-1 or L1)...
// BeeShin Tan 1 , Matthew Anaka Siddhartha Deb 2 Claudia Freyer Lisa M. Ebert 3 Anderly C. Chueh Sheren Al-Obaidi Andreas Behren Aparna Jayachandran Jonathan Cebon Weisan Chen 4 and John Mariadason Ludwig Institute for Cancer Research Ltd. Melbourne-Austin Branch, Heidelberg, Victoria, Australia. Department of Pathology, Peter MacCallum Centre, Melbourne, Centre Biology, SA Frome Road, Adelaide, School Molecular Science, LaTrobe University, Bundoora, Correspondence: Mariadason, email: Chen,...
Abstract Patients diagnosed with triple negative breast cancer (TNBC) have an increased risk of rapid metastasis compared to other subtypes. Predicting long-term survival post-chemotherapy in patients TNBC is difficult, yet enhanced infiltration tumor infiltrating lymphocytes (TILs) has been associated therapeutic response and reduced metastatic relapse. Immune biomarkers that predict the immune state a relapse pre- or mid-neoadjuvant chemotherapy are urgently needed allow earlier...
Stratification of patients for treatment ductal carcinoma in situ (DCIS) is suboptimal, with high systemic overtreatment rates. A training set 95 tumours from women pure DCIS were immunostained proteins involved cell survival, hypoxia, growth factor and hormone signalling. generalised linear regression regularisation variable selection was applied to a multiple covariate Cox survival analysis recurrence-free 10-fold cross-validation leave-one-out iterative approach used build test the model...
Metastatic disease is the major cause of breast cancer-related death and despite many advances, current therapies are rarely curative. Tumor cell migration invasion require actin cytoskeletal reorganization to endow cells with capacity disseminate initiate formation secondary tumors. However, it still unclear how these migratory colonize distant tissues form macrometastases. The E6-associated protein, E6AP, acts both as an E3 ubiquitin-protein ligase a coactivator steroid hormone receptors....
Abstract Purpose: Cancer cells recapitulate many behaviors of pluripotent embryonic such as unlimited proliferation, and the capacity to self-renew migrate. Embryo-cancer sequence A (ECSA), later named developmental pluripotency associated-2 (DPPA2), is an gene initially isolated from human preimplantation embryos. We hypothesized that ECSA/DPPA2 would be quiescent in most normal tissues but expressed cancers may therefore a useful target for immunotherapy. Experimental Design: expression...
Microcystic adnexal carcinoma (MAC) is a rare cutaneous tumour where the role of radiotherapy remains undefined. We contrast our institutional experience with current literature, define local control rate and suggest for adjuvant in treatment this cancer.From 1992-2008, retrospective review was undertaken MAC treated curative intent involving surgery at Royal Brisbane Hospital Mater Brisbane. Clinical, pathologic details as well patterns recurrence were analysed.Fourteen cases received...
Abstract Introduction Although a substantial proportion of male breast cancers (MBCs) are hereditary, the molecular pathways that activated unknown. We therefore examined frequency and clinicopathological associations PIK3CA/mammalian target rapamycin (mTOR) mitogen-activated protein kinase (MAPK) their regulatory genes in familial MBC. Methods High resolution melting analysis confirmatory sequencing was used to determine presence somatic mutations PIK3CA (exon 9 20), AKT1 4), KRAS 2) BRAF...
Aims GATA‐binding protein 3 (GATA3) is a well‐studied transcription factor found to be essential in the development of luminal breast epithelium and has been identified variety tumour types, including urothelial carcinomas, making it useful immunohistochemistry marker diagnosis both primary metastatic disease. Methods results We investigated GATA3 expression 106 triple‐negative carcinomas (100 basal‐like, six non‐basal‐like) using Cell Marque mouse monoclonal anti‐GATA3 (L50‐823). Reverse...
DNA methylation is a well-studied biomarker in invasive breast cancer, but its role ductal carcinoma situ (DCIS) less well characterized. The aims of this study are to assess the profile DCIS for panel well-characterized genes that frequently methylated investigate relationship with pathological features, and perform proof-of-principle evaluate practicality as diagnostic material.Promoter CpG island 11 cancer-related was performed by methylation-sensitive high resolution melting (MS-HRM)....
Prostate cancer (PC) is the most common in men. Elevated levels of E3 ligase, E6-Associated Protein (E6AP) were previously linked to PC, consistent with increased protein expression a subset PC patients. In cancers, irregular ligase activity drives proteasomal degradation tumor suppressor proteins. Accordingly, inhibitors define rational therapy restore suppression. The relevant suppressors targeted by E6AP are yet be fully identified. this study we show that p27, key cell cycle regulator,...
Abstract Aim The primary aim of this study was to examine lymph node status after neoadjuvant chemoradiotherapy ( CRT ) using a novel scoring system describing the pathological regression grade. proposed based on percentage fibrosis and presence residual tumour amount. secondary assess oncological impact system. Method project retrospective cohort over 10‐year period. Two hundred two patients with rectal cancer who had received followed by curative surgery were included. A histopathologist...