A5051764063- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Advanced Breast Cancer Therapies
- Histone Deacetylase Inhibitors Research
- Protein Degradation and Inhibitors
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
- SARS-CoV-2 and COVID-19 Research
- Cancer-related gene regulation
- Microtubule and mitosis dynamics
- Cancer, Hypoxia, and Metabolism
- Fibroblast Growth Factor Research
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Breast Cancer Treatment Studies
- Nuts composition and effects
- Erythrocyte Function and Pathophysiology
- Respiratory viral infections research
- Cancer Treatment and Pharmacology
- Rabies epidemiology and control
- Developmental Biology and Gene Regulation
- Coconut Research and Applications
- Neurobiology and Insect Physiology Research
- RNA and protein synthesis mechanisms
- Infectious Encephalopathies and Encephalitis
Australian Centre for Disease Preparedness
2020-2024
The University of Queensland
2022-2024
Commonwealth Scientific and Industrial Research Organisation
2020-2024
Australian e-Health Research Centre
2023
Commonwealth Foundation
2023
Peter MacCallum Cancer Centre
2010-2019
La Trobe University
2017
Cancer Genetics (United States)
2017
The University of Melbourne
2005-2016
University of Turin
2012
Here we describe a novel protein, which have named Tanis, that is implicated in type 2 diabetes and inflammation. In Psammomys obesus, unique polygenic animal model of the metabolic syndrome, Tanis expressed liver inverse proportion to circulating glucose (P = 0.010) insulin levels 0.004) direct with plasma triglyceride concentrations 0.007). Hepatic gene expression was markedly increased (3.1-fold) after 24-h fast diabetic but not nondiabetic P. obesus. addition, inhibited cultured...
Polarity coordinates cell movement, differentiation, proliferation and apoptosis to build maintain complex epithelial tissues such as the mammary gland. Loss of polarity deregulation these processes are critical events in malignant progression but precisely how at which stage loss impacts on development tumourigenesis is unclear. Scrib a core regulator tumour suppressor gene however date our understanding function gland has been limited culture transplantation studies lines. Utilizing...
Abstract Sarbecoviruses such as SARS and SARS-CoV-2 have been responsible for two major outbreaks in humans, the latter resulting a global pandemic. While sarbecoviruses primarily cause an acute respiratory infection, they shown to infect nervous system. However, mechanisms of sarbecovirus neuroinvasion neuropathogenesis remain unclear. In this study, we examined infectivity trans-synaptic transmission potential human stem cell–derived neural model systems. We demonstrated limited ability...
The establishment and maintenance of polarity is vital for embryonic development loss a frequent characteristic epithelial cancers, however the underlying molecular mechanisms remain unclear. Here, we identify novel role protein Scrib as mediator epidermal permeability barrier acquisition, skeletal morphogenesis, potent tumor suppressor in cutaneous carcinogenesis. To explore during development, compared toluidine blue dye wild-type, heterozygous KO epidermis at E16.5, E17.5 E18.5. Mouse...
Scribble is a highly conserved protein regulator of cell polarity that has been demonstrated to function as tumor suppressor or, conversely, an oncogene in context-dependent manner, and it also controls many physiological processes ranging from immunity memory. consists leucine-rich repeat domain four PDZ domains, with the latter being responsible for most Scribble's complex formation other proteins. Given similarities sequences their binding grooves, common these domains show overlapping...
Metastatic disease is the major cause of breast cancer-related death and despite many advances, current therapies are rarely curative. Tumor cell migration invasion require actin cytoskeletal reorganization to endow cells with capacity disseminate initiate formation secondary tumors. However, it still unclear how these migratory colonize distant tissues form macrometastases. The E6-associated protein, E6AP, acts both as an E3 ubiquitin-protein ligase a coactivator steroid hormone receptors....
To study the expression and function of brain-specific proteinase deficient disintegrins, ADAM11 ADAM22 (a disintegrin metalloproteinase).Specimens low- high-grade gliomas normal brain were analyzed for using Western blotting. The effects overexpression in glioma cells on growth bromodeoxyuridine incorporation linked to immunocytochemistry. Similarly cell proliferation bacterially expressed glutathione S-transferase fusion proteins with domain ADAM22.ADAM22 is some low-grade gliomas, but not...
The repurposing of licenced drugs for use against COVID-19 is one the most rapid ways to develop new and alternative therapeutic options manage ongoing pandemic. Given circa 7817 compounds available from Compounds Australia that can be screened, this paper demonstrates utility commercially ex vivo/3D airway alveolar tissue models. These models are a closer representation in vivo studies than vitro models, but retain benefits screening drug efficacy. We demonstrate several existing appear...
ADAM22 is one of three catalytically inactive ADAM family members highly expressed in the brain. has numerous splice variants, all with considerable cytoplasmic tails up to 148 amino acids. can act inhibit cell proliferation, however, it been suggested that also acts as an adhesion protein. We identified 14-3-3 protein by a yeast two-hybrid screen and show co-immunoprecipitation domain interact six proteins In addition, we preferentially serine phosphorylated precursor form ADAM22. two...
Rabies is a zoonotic neurological infection caused by lyssavirus that continues to result in devastating loss of human life. Many aspects rabies pathogenesis neurons are not well understood. Lack appropriate ex-vivo models for studying has contributed this knowledge gap. In study, we utilize advances stem cell technology characterize cell-derived neurons. We show key cellular features our neural cultures, including upregulation inflammatory chemokines, lack neuronal apoptosis, and axonal...
Abstract The VANGL family of planar cell polarity proteins is implicated in breast cancer however its function mammary gland biology unknown. Here, we utilized a panel Vang1 and Vangl2 mouse alleles to examine the requirement members murine gland. We show that Vang1CKO Δ/Δ glands display normal branching while flox/flox Lp/Lp tissue exhibit several phenotypes. In MMTV - Cre;Vangl2 glands, turnover reduced lumens are narrowed. A missense mutation leads anlage sprouting defects deficient...
Erythroid enucleation is critical for terminal differentiation of red blood cells, and involves extrusion the nucleus by orthochromatic erythroblasts to produce reticulocytes. Due difficulty synchronizing erythroblasts, molecular mechanisms underlying process remain poorly understood. To elucidate cellular program governing enucleation, we utilized a novel chemical screening approach whereby cells primed were enriched ex vivo subjected functional drug screen using 324 compound library...
Despite being vaccine preventable, rabies (lyssavirus) still has a significant impact on global mortality, disproportionally affecting children under 15 years of age. This neurotropic virus is deft at avoiding the immune system while travelling through neurons to brain. Until recently, research efforts into role non-coding RNAs in pathogenicity and detection have been hampered by lack human vitro neuronal models. Here, we utilized our previously described stem cell-derived neural model...
Women with high mammographic density (MD) are at increased risk of breast cancer (BC) after adjustment for age and body mass index. We have developed a murine biochamber model in which both MD (HMD) low (LMD) tissue can be propagated. Here, we tested whether cells isolated by collagenase digestion fluorescence-activated cell sorting (FACS) from normal reconstituted our model, would allow cell-specific manipulations to tested. Fresh was collected women (n = 7) undergoing prophylactic...
Abstract Sarbecoviruses such as SARS and SARS-CoV-2 have been responsible for two major outbreaks in humans, the latter resulting a global pandemic. While sarbecoviruses primarily cause an acute respiratory infection, they shown to infect nervous system. However, mechanisms of sarbecovirus neuroinvasion neuropathogenesis remain unclear. In this study, we examined infectivity trans-synaptic transmission potential human stem cell-derived neural model systems. We demonstrated limited ability...
Erythroid enucleation is the process by which future red blood cell disposes of its nucleus prior to entering stream. This key event during development has been likened an asymmetric division (ACD), enucleating erythroblast divides into two very different daughter cells alternate molecular composition, a nucleated that will be removed associated macrophages, and reticulocyte mature definitive erythrocyte. Here we investigated gene expression members Par, Scribble Pins/Gpsm2 complexes in...
Abstract The novel coronavirus disease (COVID-19) and any future outbreaks will require more affordable, effective safe treatment options to complement current ones such as Paxlovid . Drug repurposing can be a promising approach if we are able find rapid, robust reliable way down-select screen candidates using in silico vitro approaches. With repurposed drugs, ex vivo models could offer rigorous route human clinical trials with less time invested into nonclinical animal ( ) studies. We have...
The repurposing of licenced drugs for use against COVID-19 is one the most rapid ways to develop new and alternative therapeutic options manage ongoing pandemic. Given approximately 8,000 compounds available from Compounds Australia that can be screened, this paper demonstrates utility commercially-available ex vivo/3D airway alveolar tissue models. These models are a closer representation in vivo studies compared vitro models, but retain benefits screening drug efficacy. We demonstrate...
Abstract Metastatic disease is the major cause of breast cancer related death and despite many advances, current therapies are rarely curative. Tumor cell migration invasion require actin cytoskeletal reorganisation, to endow cells with capacity disseminate initiate formation secondary tumors. However, it still unclear how these migratory colonise distant tissues form macrometastases. The E6-associated protein, E6AP, acts both as an E3 ubiquitin-protein ligase a coactivator steroid hormone...