A5044827602- MicroRNA in disease regulation
- Pancreatic function and diabetes
- Extracellular vesicles in disease
- Diabetes and associated disorders
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- SARS-CoV-2 detection and testing
- Diabetes Management and Research
- Epigenetics and DNA Methylation
- Aquaculture Nutrition and Growth
- Cancer Genomics and Diagnostics
- Physiological and biochemical adaptations
- Rabies epidemiology and control
- Viral Infections and Vectors
- Biosensors and Analytical Detection
- COVID-19 Clinical Research Studies
- Venomous Animal Envenomation and Studies
- Plant Virus Research Studies
- Marine Bivalve and Aquaculture Studies
- Autophagy in Disease and Therapy
- Streptococcal Infections and Treatments
- Pancreatitis Pathology and Treatment
Australian Centre for Disease Preparedness
2019-2025
CSIRO Health and Biosecurity
2021-2025
Western Sydney University
2021-2024
Camden and Campbelltown Hospitals
2022-2023
Commonwealth Scientific and Industrial Research Organisation
2019-2023
The University of Sydney
2013-2021
Health Sciences and Nutrition
2021
National Health and Medical Research Council
2013-2021
The host response to SARS-CoV-2 infection provide insights into both viral pathogenesis and patient management. host-encoded microRNA (miRNA) infection, however, remains poorly defined. Here we profiled circulating miRNAs from ten COVID-19 patients sampled longitudinally age gender matched healthy donors. We observed 55 that were altered in during early-stage disease, with the inflammatory miR-31-5p most strongly upregulated. Supervised machine learning analysis revealed a three-miRNA...
Abstract MicroRNAs are now increasingly recognized as biomarkers of disease progression. Several quantitative real-time PCR (qPCR) platforms have been developed to determine the relative levels microRNAs in biological fluids. We systematically compared detection cellular and circulating microRNA using a standard 96-well platform, high-content microfluidics platform two ultra-high content platforms. used extensive analytical tools compute inter- intra-run variability concordance measured...
Abstract The host response to SARS-CoV-2 infection provide insights into both viral pathogenesis and patient management. host-encoded microRNA (miRNA) infection, however, remains poorly defined. Here we profiled circulating miRNAs from ten COVID-19 patients sampled longitudinally age gender matched healthy donors. We observed 55 that were altered in during early-stage disease, with the inflammatory miR-31-5p most strongly upregulated. Supervised machine learning analysis revealed a...
Abstract The aim of this cross-sectional study was to compare plasma C-peptide presence and levels in people without diabetes (CON) with Type 1 relate status clinical factors. In a subset we evaluated 50 microRNAs (miRs) previously implicated beta-cell death associations levels. Diabetes age onset stratified as adult (≥ 18 y.o) or childhood (< y.o.), duration ≤ 10 years, 10–20 years > 20 years. Plasma measured by ultrasensitive ELISA. miRs were quantified using TaqMan probe-primer mix...
Host biomarkers are increasingly being considered as tools for improved COVID-19 detection and prognosis. We recently profiled circulating host-encoded microRNA (miRNAs) during SARS-CoV-2 infection, revealing a signature that classified cases with 99.9% accuracy. Here we sought to develop suited clinical application by analyzing specimens collected using minimally invasive procedures. Eight miRNAs displayed altered expression in anterior nasal tissues from patients, miR-142-3p, negative...
Changing ocean temperatures are already causing declines in populations of marine organisms. Predicting the capacity organisms to adjust pressures posed by climate change is a topic much current research effort, particularly for species we farm or harvest. To explore one measure phenotypic plasticity, physiological compensations response heat stress as might be experienced heatwave, exposed Yellowtail Kingfish (Seriola lalandi) sublethal stress, and used transcriptome gill muscle,...
Maintaining animal welfare is an essential component of production systems. However, multiple measurements are required to inform animal's state as there currently no universal measurement tools. Novel biomarkers increasingly being explored measures stress, pain and disease status in livestock. Here we investigate host-encoded microRNAs (miRNAs) stress determine the Australian Merino sheep (Ovis aries) following castration tail-docking. This study used samples from a retrospective trial that...
Abstract Understanding the intricate nexus between cattle health and microbiome dynamics holds profound implications for enhancing animal productivity welfare. However, our understanding of role these microbial communities is limited in beef cattle, especially understudied body sites such as oral nasal microbiome. Here, using a genome-centric metagenomics approach, we recovered substantial metagenome-assembled genomes (MAGs) from faecal, Australian Angus different herds life stages. The MAGs...
Abstract An immune competence (IC) trait has been developed in livestock to combat infectious diseases through selective breeding. Here, we investigate whether circulating host-encoded microRNAs (miRNAs) are associated with responses a commercial multivalent clostridial and leptospiral vaccine Australian Angus steers, proxy measure for IC. A total of 332 animals from two herds New South Wales, Australia—Herd 1 (n = 168) Herd 2 164)—were IC phenotyped on the day yard weaning. Within herd,...
Probe-based quantitative PCR (qPCR) is a favoured method for measuring transcript abundance, since it one of the most sensitive detection methods that provides an accurate and reproducible analysis. chemistry offers least background fluorescence as compared to other (dye-based) chemistries. Presently, there are several platforms available use probe-based quantitate abundance. qPCR in 96 well plate routinely used method, however only maximum samples or miRNAs can be tested single run. This...
Dicer knockout mouse models demonstrated a key role for microRNAs in pancreatic β-cell function. Studies to identify specific microRNA(s) associated with human (pro-)endocrine gene expression are needed. We profiled and genes 353 tissue samples. Machine learning workflows identified (pro-)insulin transcripts discovery set of islets (n = 30) insulin-negative tissues 62). This microRNA signature was validated remaining 261 that include nine islet samples from individuals type 2 diabetes. Top...
Rabies is a zoonotic neurological infection caused by lyssavirus that continues to result in devastating loss of human life. Many aspects rabies pathogenesis neurons are not well understood. Lack appropriate ex-vivo models for studying has contributed this knowledge gap. In study, we utilize advances stem cell technology characterize cell-derived neurons. We show key cellular features our neural cultures, including upregulation inflammatory chemokines, lack neuronal apoptosis, and axonal...
Probe-based quantitative PCR (qPCR) is a favoured method for measuring transcript abundance, since it one of the most sensitive detection methods that provides an accurate and reproducible analysis. chemistry offers least background fluorescence as compared to other (dye-based) chemistries. Presently, there are several platforms available use probe-based quantitate abundance. qPCR in 96 well plate routinely used method, however only maximum samples or miRNAs can be tested single run. This...
Despite being vaccine preventable, rabies (lyssavirus) still has a significant impact on global mortality, disproportionally affecting children under 15 years of age. This neurotropic virus is deft at avoiding the immune system while travelling through neurons to brain. Until recently, research efforts into role non-coding RNAs in pathogenicity and detection have been hampered by lack human vitro neuronal models. Here, we utilized our previously described stem cell-derived neural model...
The burgeoning field of miRNA detection using electrochemiluminescence (ECL) has garnered a great deal attention in recent times. 1 ECL presents many advantages over alternative strategies as analyses generally exhibit low-background signals, high sensitivity, are reproducible and scale-able. 2 When combined with magnetic-beads for the immobilisation biological recognition elements, biosensors offer exceptional sensitivity owing to ability disperse beads large sample volumes capture target...
Circulating cell-free DNA (cfDNA) has been intensively investigated as a diagnostic and prognostic marker for various cancers. In recent years, presence of unmethylated insulin cfDNA in the circulation correlated with pancreatic &beta;-cell death risk developing type 1 diabetes. Digital (d)PCR is an increasingly popular method quantifying due to its ability determine absolute copy numbers, increased sensitivity when compared more routinely used quantitative PCR. Multiple platforms have...
<title>Abstract</title> Identifying biomarkers of functional β-cell loss is critical in risk stratification for Type 1 Diabetes (T1D). We report a microRNA-based dynamic (responsive to environment) score developed using multi-center, multi-ethnic/country (“multi-context”) cohorts. Discovery (wet-lab and dry-lab) analysis identified 50 microRNAs that were measured across n=2,204 individuals from four contexts (4C=AUS/Australia, DNK/Denmark, HKG/Hong Kong SAR China, IND/India). A (DRS) was...
Ever since the discovery of small non-coding RNAs, microRNAs have been identified to play a critical role in development and function pancreatic insulin-producing beta cells.Research carried out until now demonstrates that can specifically target key transcription factors signalling molecules.This turn may influence changes insulin production secretion.microRNAs are also organs altered as result hyperglycemia resistance.Recent studies demonstrate not only confined cells but detected...