A5041144405- Mosquito-borne diseases and control
- Nuclear Structure and Function
- Pancreatic function and diabetes
- TGF-β signaling in diseases
- Diabetes and associated disorders
- Protease and Inhibitor Mechanisms
- Diabetes Management and Research
- Protein Kinase Regulation and GTPase Signaling
- Proteoglycans and glycosaminoglycans research
- RNA Research and Splicing
- HIV Research and Treatment
- Ion channel regulation and function
- Insect symbiosis and bacterial influences
- Studies on Chitinases and Chitosanases
- interferon and immune responses
- Wnt/β-catenin signaling in development and cancer
- Viral Infections and Immunology Research
- Dermatological diseases and infestations
- Bird parasitology and diseases
- Virology and Viral Diseases
- Metabolism, Diabetes, and Cancer
- Liver physiology and pathology
- Glycosylation and Glycoproteins Research
- Genomics and Chromatin Dynamics
- Viral Infections and Vectors
Monash University
2009-2022
Western Sydney University
2021-2022
Camden and Campbelltown Hospitals
2022
O'Brien Institute
2011
The University of Melbourne
2011
Baker Heart and Diabetes Institute
2009-2010
The University of Queensland
2010
Growth factors modify the structure of glycosaminoglycan (GAG) chains on biglycan leading to enhanced LDL binding. G-protein receptor-coupled agonists such as thrombin, signal changes proteoglycans produced by vascular smooth muscle cells (VSMCs). One component classical G-protein-coupled receptor (GPCR) signaling invokes transactivation protein tyrosine kinase receptors epidermal growth factor receptor. Serine/threonine transforming factor-(TGF)-β are potent activators proteoglycan...
Importin-alpha is the nuclear import receptor that recognizes classic monopartite and bipartite localization sequences (cNLSs), which contain one or two clusters of basic amino acids, respectively. Different importin-alpha paralogs in a single organism are specific for distinct repertoires cargos. Structural studies revealed cNLSs C-terminal bind to same site on importin-alpha, termed major cNLS-binding site. We used an oriented peptide library approach with five degenerate positions probe...
Dengue virus (DENV) threatens almost 70% of the world’s population, with no effective vaccine or therapeutic currently available. A key contributor to infection is nuclear localisation in infected cell DENV nonstructural protein 5 (NS5) through action host importin (IMP) α/β1 proteins. Here, we used a range microscopic, virological and biochemical/biophysical approaches show for first time that small molecule GW5074 has anti-DENV its novel ability inhibit NS5–IMPα/β1 interaction vitro as...
Viral disease is one of the greatest burdens for human health worldwide, with an urgent need efficacious antiviral strategies. While drugs are available, in many cases, they prone to development drug resistance. A way overcome resistance associated common therapies develop antivirals targeting host cellular co-factors critical viral replication, such as DEAD-box helicase 3 X-linked (DDX3X), which plays key roles RNA metabolism and response. Here, we use biochemical/biophysical approaches...
Dengue virus (DENV) threatens almost 70% of the world's population, with no effective therapeutic currently available and controversy surrounding one approved vaccine. A key factor in dengue viral replication is interaction between DENV nonstructural proteins (NS) 5 3 (NS3) infected cell. Here, we perform a proof-of-principle high-throughput screen to identify compounds targeting NS5-NS3 binding interface. We use range approaches show for first time that two small molecules-repurposed drugs...
DEAD-box helicase 3, X-linked (DDX3X) regulates the retinoic acid-inducible gene I (RIG-I)-like receptor (RLR)-mediated antiviral response, but can also be a host factor contributing to replication of viruses significance human health, such as immunodeficiency virus type 1 (HIV-1). These roles are mediated in part through its ability actively shuttle between nucleus and cytoplasm modulate expression, although trafficking mechanisms, impact thereof on immune signaling viral infection,...
The mechanism through which growth factors cause glycosaminoglycan (GAG) hyper-elongation is unclear. We have investigated the role of transcription and translation on GAG effect plateletderived factor (PDGF) in human vascular smooth muscle cells (VSMCs). To determine if response involves specific signalling pathways or process we also effects epidermal (EGF), transforming factor-β (TGF-β) thrombin. report that both actinomycin D cycloheximide completely abolished ability PDGF to stimulate...
Atherosclerosis and its sequelae remain the major contributors to morbidity premature mortality, despite availability of efficacious statin drugs. It has recently been demonstrated that human atherosclerosis in coronary arteries commences with binding retention atherogenic ApoB-containing lipoproteins by modified proteoglycans. Vascular smooth muscle cells are source proteoglycans vessel wall. A multitude vasoactive factors stimulate synthesis modify structure glycosaminoglycan chains...
Abstract Objective Pancreatic islet β-cells are factories for insulin production; however ectopic expression of is also well recognized. The gallbladder a next-door neighbour to the developing pancreas. Here, we wanted understand if gallbladders contain functional insulin-producing cells. Design We compared and adult mouse as human epithelial cells islets using immunohistochemistry, flow cytometry, ELISAs, RNA-sequencing, real-time PCR, chromatin immunoprecipitation studies. Results...