A5091410649- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- MicroRNA in disease regulation
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Circular RNAs in diseases
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Gut microbiota and health
- Vaccine Coverage and Hesitancy
- Immunodeficiency and Autoimmune Disorders
- Renal Transplantation Outcomes and Treatments
- Cancer Genomics and Diagnostics
- Prenatal Screening and Diagnostics
- Antifungal resistance and susceptibility
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Blood disorders and treatments
- Viral Infections and Outbreaks Research
- Renal and related cancers
- Immunotherapy and Immune Responses
- Clostridium difficile and Clostridium perfringens research
- Chronic Lymphocytic Leukemia Research
- Molecular Biology Techniques and Applications
Semnan University of Medical Sciences
2024-2025
Tehran University of Medical Sciences
2019-2023
National Institute of Genetic Engineering and Biotechnology
2021
Abstract Conventional cancer treatments can cause serious side effects because they are not specific to cells and damage healthy cells. Aptamers often single-stranded oligonucleotides arranged in a unique architecture, allowing them bind specifically target sites. This feature makes an ideal choice for targeted therapeutics. They typically produced through the systematic evolution of ligands by exponential enrichment (SELEX) undergo extensive pharmacological revision modify their affinity,...
Aim: Cell-free DNA in the plasma is known to be a potential biomarker for noninvasive diagnosis of oncogenic mutations. The authors aimed design an optimized padlock probe-based hyperbranched rolling circle amplification biosensor detect KRAS G12D mutation using fluorescence and colorimetric methods. Methods: Single-factor experiments, Plackett–Burman response surface methodology were applied optimize reaction. Results: maximum intensity was achieved at probe concentration 1.5 pM target 9...
Background: DNA probes have been widely used as diagnostic tools for translocations. This study sought to design a screening tool using ssDNA and chromosome conformation capture (3C) library fragment hybridization. Method: The authors focused on developing probe the juxtaposed region of MYC TRD. Fragments gene with thiol modification (MYC–Au NP probe) were functionalized by gold nanoparticles (Au NPs). Then TRD immobilized nitrocellulose surface. Hybridization between 3C fragments SKW3 cells...
Abstract Background DNA probes have been widely used as diagnostic tools for chromosomal translocations in malignancies. PCR-based methods often fail to detect such MYC/TRD chronic lymphocytic leukemia. In addition, microscopic techniques cannot be helpful due size detection limitations. This study sought design a screening tool using immobilized ssDNA on nitrocellulose membrane followed by 3C library fragments hybridization. Results Hence, we focused developing suitable 27 bp specific probe...
Abstract Background: DNA probes have been widely used as diagnostic tools for chromosomal translocations in malignancies. PCR-based methods often fail to detect such MYC/TRD chronic lymphocytic leukemia. In addition, microscopic techniques cannot be helpful due size detection limitations. This study sought design a screening tool using immobilized ssDNA on nitrocellulose membrane followed by 3C library fragments hybridization. Results: Hence, we focused developing suitable 27 bp specific...