A5009850644- Cancer, Lipids, and Metabolism
- RNA Research and Splicing
- Peroxisome Proliferator-Activated Receptors
- Cancer Cells and Metastasis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Lipoproteins and Cardiovascular Health
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Cholesterol and Lipid Metabolism
- RNA and protein synthesis mechanisms
- Drug Transport and Resistance Mechanisms
- Colorectal Cancer Treatments and Studies
- 3D Printing in Biomedical Research
- Lipid metabolism and biosynthesis
- Adipose Tissue and Metabolism
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- Virus-based gene therapy research
- Pharmacogenetics and Drug Metabolism
- Metabolism, Diabetes, and Cancer
- Neurogenetic and Muscular Disorders Research
- Ferrocene Chemistry and Applications
- Hypothalamic control of reproductive hormones
- Sulfur Compounds in Biology
Inserm
2012-2024
Génétique, Génomique Fonctionnelle et Biotechnologies
2018-2024
Université de Bretagne Occidentale
2009-2024
Établissement Français du Sang
2020-2024
École nationale d'ingénieurs de Brest
2020-2023
Brest State A.S. Pushkin University
2022
EU Business School
2013
Centre Hospitalier Universitaire de Montpellier
2009
Université de Bourgogne
2003-2005
Centre National de la Recherche Scientifique
1998-2005
Colorectal cancer (CRC) is one of the most aggressive cancers worldwide. Several anticancer agents are available to treat CRC, but eventually relapse occurs. One major cause chemotherapy failure emergence drug-resistant tumor cells, suspected originate from stem cell compartment. The aim this study was ask whether drug resistance associated with acquisition cell-like properties. We isolated derivatives two human CRC lines, HT29 and HCT116, using drugs distinct modes action, oxaliplatin...
Abstract Radiogenomics aims at investigating the relationship between imaging radiomic features and gene expression alterations. This study addressed potential prognostic complementary value of contrast enhanced computed tomography (CE-CT) data in primary colorectal cancers (CRC). Sixty-four patients underwent CT scans were extracted from delineated tumor volume. Gene analysis a small set genes, previously identified as relevant for CRC, was conducted on surgical samples same tumors. The...
Abstract Gastric cancer (GC) is the third cause of cancer-related mortality worldwide and often diagnosed at advanced stages disease. This makes development more comprehensive models efficient treatments crucial. One option based on repurposing already marketed drugs as adjuvants to chemotherapy. Accordingly, we have previously developed combination docetaxel cholesterol-lowering drug, lovastatin, a powerful trigger HGT-1 human GC cells’ apoptosis using 2D cultures. Because 3D models, known...
Dietary vegetable oils and fish rich in PUFA (polyunsaturated fatty acids) exert hypocholesterolaemic hypotriglyceridaemic effects rodents. The plasma cholesterol-lowering properties of are due partly to a diminution cholesterol synthesis the activity rate-limiting enzyme HMG-CoA reductase (3-hydroxy-3-methylglutaryl-CoA reductase). To better understand mechanisms involved, we examined how tuna oil individual n−3 n−6 affect expression hepatic FPP synthase (farnesyl diphosphate synthase),...
Microsatellite instability (MSI) due to mismatch repair deficiency (dMMR) is common in colorectal cancer (CRC). These cancers are associated with somatic coding events, but the noncoding pathophysiological impact of this genomic yet poorly understood. Here, we perform an analysis and MSI events at different steps tumorigenesis using whole exome sequencing search for splicing via RNA bulk-tumor single-cell levels. Our results demonstrate that leads hundreds DNA mutations, notably...
Recombinant adenoviruses are widely used for the transfer of foreign genes into various mammalian cells. However, utilization these vectors cancer gene therapy requires specific and efficient expression transferred in tumor To obtain targeted hepatoma cells, we constructed recombinant adenoviral containing transcriptional elements from either rat α-fetoprotein (AFP) or human insulin-like growth factor II (IGFII) driving nuclear β-galactosidase (nls lacZ). In vitro infection revealed that AFP...
Cancer cell killing might be achieved by the combined use of available drugs. Statins are major anti-hypercholesterolemia drugs, which also trigger apoptosis many cancer types, while docetaxel is a potent microtubule-stabilising agent. Here, we looked at effects lovastatin and in cells. Whole transcriptome microarrays HGT-1 gastric cells demonstrated that strongly suppressed expression genes involved division, had very little transcriptional effects. Both drugs triggered apoptosis, their...
Gastric cancer (GC) is highly deadly. Three-dimensional (3D) cell cultures, known as spheroids, better mimic tumor microenvironment (TME) than standard 2D cultures. Cancer-associated fibroblasts (CAF), a major cellular component of TME, promote or restrain proliferation, invasion and resistance to drugs. We established spheroids from two human GC lines mixed with primary CAF. Spheroid organization, analyzed by two-photon microscopy, showed CAF in AGS/CAF clustered the center, but dispersed...
By using degenerate primers designed from glutamate decarboxylase (GAD) sequences of mammals, Xenopus and Drosophila, a 270-bp cDNA fragment was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) cerebellum total RNA rainbow trout. This partial shows 90% identity with mammalian GAD 65 presents the Asn-Pro-His-Lys (NPHK) sequence corresponding to pyridoxal-binding region porcine DOPA or GAD. The distribution mRNA-expressing neurons in forebrain trout studied situ hybridization...
Caspases play important roles in apoptotic cell death and some other functions, such as cytokine maturation, inflammation, or differentiation. We show here that the 5'-flanking region of human CASP-2 gene contains three functional response elements for sterol regulatory element binding proteins (SREBPs), mediate transcriptional activation genes involved cholesterol, triacylglycerol, fatty acid synthesis. Exposure several lines to statins, lipid-lowering drugs drive SREBP proteolytic...
Statins are lipid-lowering drugs that may help limit cancer occurrence in humans. They drive blockage of the mevalonate pathway, trigger cell apoptosis vitro and reduce tumour incidence animals. We have shown present study statins induced HGT-1 human gastric cells, this was prevented by intermediates cholesterol synthetic pathway. In addition, similarly to what we reported previously for caspase 2 [Logette, Le Jossic-Corcos, Masson, Solier, Sequeira-Legrand, Dugail, Lemaire-Ewing, Desoche,...
Cystic fibrosis is a prominent genetic disease caused by mutations of the cystic transmembrane conductance regulator (CFTR) gene. Among many disease-causing alterations are pre-mRNA splicing defects that can hamper mandatory exon inclusion. CFTR 9 depends in part on polymorphic UG(m)U(n) sequence at end intron 8, which be bound TDP-43, leading to partial skipping. CELF proteins, like CUG-BP1 and ETR-3, also bind UG repeats regulate splicing. We show here but not CUG-BP1, strongly stimulates...
A panel of four novel human hepatoma cell lines was isolated from a single tumor male individual. BC1, B16 and B16A2 were well differentiated, while cells the B9 line only poorly being essentially negative for functions analyzed. These have been surveyed expression large set plasma proteins, accumulation liver‐specific mRNAs DNA‐binding activity ubiquitous liver‐enriched transcription factors. BC1 expressed highest levels albumin mRNA, whereas accumulated largest amounts haptoglobin mRNA. In...
Abstract We have previously shown that the combination of statins and taxanes was a powerful trigger HGT-1 human gastric cancer cells’ apoptosis 1 . Importantly, several genes involved in “Central carbon metabolism pathway cancer”, as reported Kyoto Encyclopedia Genes Genomes, were either up- ( ACLY , ERBB2, GCK, MYC, PGM, PKFB2, SLC1A5, SLC7A5, SLC16A3,) or down- (IDH, MDH1, OGDH, P53, PDK ) regulated response to drug association. In present study, we conducted non-targeted metabolomics...