A5071657898- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Enzyme Structure and Function
- Geological and Geochemical Analysis
- Catalytic C–H Functionalization Methods
- Metal-Organic Frameworks: Synthesis and Applications
- High-pressure geophysics and materials
- ATP Synthase and ATPases Research
- Geochemistry and Geologic Mapping
- Biochemical and Molecular Research
- earthquake and tectonic studies
- Cyclopropane Reaction Mechanisms
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Crystallography and molecular interactions
- Amino Acid Enzymes and Metabolism
- Fluorine in Organic Chemistry
- Immune Response and Inflammation
- Protein Structure and Dynamics
- Sulfur-Based Synthesis Techniques
- Magnesium Alloys: Properties and Applications
- Synthetic Organic Chemistry Methods
- Geochemistry and Elemental Analysis
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
South China Normal University
2018-2025
Occidental College
2024
China University of Geosciences
2007-2024
Chengdu University
2018-2024
Chengdu University of Traditional Chinese Medicine
2017-2024
Ministry of Agriculture and Rural Affairs
2023-2024
Guangdong Academy of Agricultural Sciences
2023-2024
Shanghai Jiao Tong University
2008-2023
Renji Hospital
2023
Huaqiao University
2019-2023
Significance The Toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD-2) complex recognizes lipopolysaccharide (LPS) on Gram-negative bacteria to induce an innate immune response. Neoseptins, chemically synthesized peptidomimetics that bind and activate the mouse TLR4 (mTLR4)/MD-2 independent of LPS, were discovered through unbiased screening reverse genetic studies, improved by chemical modification. NMR X-ray crystallography TLR4/MD-2/Neoseptin-3 determined mechanism which...
Significance Here, we examine the activity profile of haloalkanoic acid dehalogenase (HAD) superfamily by screening a customized library against >200 enzymes from broad sampling superfamily. From this dataset, can infer function nearly 35% Overall, was found to show high substrate ambiguity, with 75% utilizing greater than five substrates. In addition, HAD members least amount structural accessorization Rossmann fold were be most specific, suggesting that elaboration core domain may have...
Abstract Although chemical and enzymatic catalysts have been combined, reactions in which an organometallic catalyst a metalloenzyme work cooperatively to create products, cannot be generated with either alone or comparable yields by sequential of the two catalysts, not reported. Such are challenging achieve, part because milieu these operate typically different. Herein, classes demonstrated react same system. Combination metathesis P450 enzyme lead dynamic equilibration alkenes selective...
Membrane-embedded prenyltransferases from the UbiA family catalyze Mg2+-dependent transfer of a hydrophobic polyprenyl chain onto variety acceptor molecules and are involved in synthesis that mediate electron transport, including Vitamin K Coenzyme Q. In humans, missense mutations to protein prenyltransferase domain-containing 1 (UBIAD1) responsible for Schnyder crystalline corneal dystrophy, which is genetic disease causes blindness. Mechanistic understanding this enzymes has been hampered...
Conversion of cellulosic biomass to renewable chemicals such as 5-hydroxymethylfurfural (HMF) is high current interest. Herein, we report a rare example one-pot synthesis HMF from glucose by tandem catalysis. The system composed thermophilic isomerase enzyme for isomerization fructose and solid acid catalyst dehydration HMF. A base (−NH2) functionalized mesoporous silica (aminopropyl-FMS) with large pore size was deployed successfully immobilize protect the in organic solvents at...
An acylative Suzuki-type cross-coupling of alkyl trifluoroborates and acid fluorides was developed by merging NHC organocatalysis with photoredox catalysis. A broad spectrum ketones could be facilely synthesised under mild reaction conditions.
Asymmetric synthesis of pharmacologically interesting piperidine-fused spiro-oxindole derivatives has been achieved via an organocatalytic Michael/aza-Henry/hemiaminalization cascade reaction. Chiral compounds synthesized by this strategy potently inhibited the proliferation several breast cancer cell lines. Mechanistic studies suggest that most potent compound 9e can directly interfere with MDM2–p53 interactions and elevate protein levels p53 p21, thereby inducing cycle arrest mitochondrial...
A three-component strategy for the synthesis of thiazoles and isothiazoles has been developed by employing enaminoesters, fluorodibromoiamides/ester, sulfur. The were formed via two C–F bond cleavages along with formation new C–S, C–N, N–S bonds. provides high selectivity thiazoles/isothiazoles, which have vital applications in drug discovery development.
2-Fluoroindoles as an important structural scaffold are widely existing in many bioactive or therapeutic agents. Despite their potential usefulness, efficient constructions of 2-fluoroindole derivatives very sparce. The development straightforward synthetic approaches to access 2-fluoroindoles is highly desirable for studying fundamental properties and applications. Herein, we report general strategy the construction which a wide variety were accessed with high efficiency chemoselectivity....
We report the synthesis of a library chiral THIQ-fused spirooxindoles. The most potent compound inhibits Ras-GTP, ultimately leading to mitochondrial apoptosis.
We present a compendium of N-ethyl-N-nitrosourea (ENU)-induced mouse mutations, identified in our laboratory over period 10 years either on the basis phenotype or whole genome and/or exome sequencing, and archived Mutagenetix database. Our purpose is threefold: 1) to formally describe many point including those that were not previously disclosed peer-reviewed publications; 2) assess characteristics these mutations; 3) estimate likelihood missense mutation induced by ENU will create...
Abstract Although chemical and enzymatic catalysts have been combined, reactions in which an organometallic catalyst a metalloenzyme work cooperatively to create products, cannot be generated with either alone or comparable yields by sequential of the two catalysts, not reported. Such are challenging achieve, part because milieu these operate typically different. Herein, classes demonstrated react same system. Combination metathesis P450 enzyme lead dynamic equilibration alkenes selective...
Abstract Although the gastrointestinal pathogen Campylobacter jejuni was considered asaccharolytic, >50% of sequenced isolates possess an operon for l -fucose utilization. In C. NCTC11168, this pathway confers chemotaxis and competitive colonization advantages in piglet diarrhea model, but catabolic steps remain unknown. Here we solved putative dehydrogenase structure, resembling FabG Burkholderia multivorans . The enzyme, FucX, reduces d -arabinose vitro both sugars are catabolized by...
Mitochondria play an important role in the energy production of plant cells through independent genetic systems. This study has aimed to assemble and annotate functions mitochondrial (mt) genome Luffa cylindrica. The mt L. cylindrica contained two chromosomes with lengths 380,879 bp 67,982 bp, respectively. Seventy-seven genes including 39 protein-coding genes, 34 tRNA 3 rRNA 1 pseudogene, were identified. About 90.63% codons ended A or U bases, 98.63% monomers A/T, which contributed high...