A5000925739- Gut microbiota and health
- Infant Nutrition and Health
- Salmonella and Campylobacter epidemiology
- Breastfeeding Practices and Influences
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Gestational Diabetes Research and Management
- Metabolomics and Mass Spectrometry Studies
- Trace Elements in Health
- Digestive system and related health
- Tryptophan and brain disorders
- Diet and metabolism studies
- Clostridium difficile and Clostridium perfringens research
- Pediatric health and respiratory diseases
- Inflammatory Bowel Disease
- Bioinformatics and Genomic Networks
- Corrosion Behavior and Inhibition
- Genomics and Rare Diseases
- Microscopic Colitis
- Probiotics and Fermented Foods
- Diet, Metabolism, and Disease
- Dermatology and Skin Diseases
- Infant Health and Development
- Helicobacter pylori-related gastroenterology studies
- Wastewater Treatment and Nitrogen Removal
University of Ottawa
2016-2025
Ottawa Institute of Systems Biology
2012-2024
Kelly Services (United States)
2020
Hampshire Hospitals NHS Foundation Trust
2017
Ipswich Hospital
2013
Queen Mary University of London
2006
Royal Hampshire County Hospital
2001-2002
Abstract Intestinal microbial dysbiosis is associated with Crohn’s disease (CD). However, the mechanisms leading to chronic mucosal inflammation that characterizes this remain unclear. In report, we use systems-level approaches study interactions between gut microbiota and host in new-onset paediatric patients evaluate causality of disease. We report an altered proteome CD indicative impaired mitochondrial functions. particular, proteins implicated H 2 S detoxification are downregulated,...
Abstract Alterations in gut microbiota have been implicated the pathogenesis of inflammatory bowel disease (IBD), however factors that mediate host–microbiota interactions remain largely unknown. Here we collected mucosal-luminal interface samples from a pediatric IBD inception cohort and characterized both human proteins using metaproteomics. We show microbial related to oxidative stress responses are upregulated cases compared controls. In particular, demonstrate expression antimicrobial...
The gut microbiota has been shown to be closely associated with human health and disease. While next-generation sequencing can readily used profile the taxonomy metabolic potential, metaproteomics is better suited for deciphering microbial biological activities. However, application of largely limited due low efficiency protein identification. Thus, a high-performance easy-to-implement metaproteomic approach required. In this study, we developed universal workflow metaproteome identification...
In a prospective double-blind controlled trial the effect of large doses ascorbic acid on healing pressure-sores has been assessed. 20 surgical patients were studied, pressure areas being assessed by serial photography and ulcer tracings. The mean ascorbic-acid levels in treated non-treated groups one month after start treatment 65.6 25.8 mug per 10-8 white blood-cells. group with there was reduction pressure-sore area 84% compared 42.7% placebo group. These findings are statistically...
Abstract Background During gut colonization, the enteric pathogen Campylobacter jejuni must surmount toxic effects of reactive oxygen species produced by its own metabolism, host immune system, and intestinal microflora. Elucidation C. oxidative stress defense mechanisms is critical for understanding pathophysiology. Results The in were characterized transcriptional profiling phenotypic analysis wild-type mutant strains. To define regulon peroxide-sensing regulator, PerR, we constructed an...
Early life microbiota is an important determinant of immune and metabolic development may have lasting consequences. The maternal gut during pregnancy or breastfeeding for defining infant microbiota. We hypothesized that a critical immunity. To test this, pregnant BALB/c dams were fed vancomycin 5 days prior to delivery (gestation; Mg), 14 postpartum nursing (Mn), gestation (Mgn), no (Mc). analyzed adaptive immunity in pups at various times after delivery. In addition direct alterations...
The full regulatory potential of the ferric uptake regulator (Fur) family proteins remains undefined despite over 20 years study. We report herein an integrated approach that combines both genome-wide technologies and structural studies to define role Fur in Campylobacter jejuni (Cj). CjFur ChIP-chip assays identified 95 genomic loci bound by associated with functions as diverse iron acquisition, flagellar biogenesis, non-iron ion transport. Comparative analysis transcriptomic data revealed...
Abstract Functional redundancy is a key ecosystem property representing the fact that different taxa contribute to an in similar ways through expression of redundant functions. The potential functions (or genome-level functional $${{{{{{\rm{FR}}}}}}}_{g}$$ <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:msub> <mml:mrow> <mml:mi>FR</mml:mi> </mml:mrow> <mml:mi>g</mml:mi> </mml:msub> </mml:math> ) human microbiomes has been recently quantified using metagenomics data. Yet,...
The composition of the intestinal microbiome seems relevant to pathogenesis Crohn's disease (CD), with differences in both diversity and gut microbiota patients CD compared healthy individuals. However, there are still conflicting reports on importance various bacterial taxa CD. aim this study was characterize mucosa-associated newly diagnosed pediatric CD.Mucosa-associated bacteria were identified from ileal biopsy specimens obtained at colonoscopy 10 either or ileocolonic new-onset 15...
Campylobacter jejuni is a frequent foodborne pathogen of humans. As C. infections commonly arise from contaminated poultry, phage treatments have been proposed to reduce the load on farms prevent human infections. While prior report documented transcriptome phages during carrier state life cycle, transcriptomic analysis lytic infection has not reported. We used RNA-sequencing profile NCTC 11168 by T4-like myovirus 12673. Interestingly, we found that most highly upregulated host genes upon...
Abstract Background Dysregulated proteolytic activity (PA) has been detected in intestinal biopsy samples of individuals with inflammatory bowel disease (IBD) compared to healthy controls. It is known that increased microbial fecal PA precedes ulcerative colitis (UC) diagnosis an at-risk population. Intestinal inflammation UC characterized by continuous ulceration, Crohn’s (CD) patchy nature. Sites CD associated altered mucosa-associated microbes and higher permeability, suggesting these...
Therapeutic compounds significantly alter the composition of gut microbiota, yet their functional and ecological impacts, particularly at protein level, remain largely unexplored. In this study, we systematically mapped metaproteomic responses ex vivo human microbiota to 312 compounds, generating 4.6 million microbial responses, available as an interactive resource (https://shiny.imetalab.ca/MPR_Viz/). Across protein-level, taxonomic composition-level, ecosystem-level analyses,...
ABSTRACT Campylobacter jejuni is a common foodborne pathogen worldwide that associated with high rates of morbidity and mortality among infants in low- to middle-income countries (LMICs). Human milk provides an important source nutrients contains antimicrobial components for protection against infection. However, recent studies, including our own, have found significantly higher levels diarrheal stool samples collected from breastfed compared non-breastfed LMICs. We hypothesized C. has...
Metabolic syndrome (MetS) constitutes a spectrum of interconnected conditions comprising obesity, dyslipidemia, hypertension, and insulin resistance (IR). While singular, all-encompassing treatment for MetS remains elusive, an integrative approach involving tailored lifestyle modifications emerging functional food therapies holds promise in preventing its multifaceted manifestations. Our main objective was to scrutinize the efficacy cranberry proanthocyanidins (PAC, 200 mg/kg/day 12 weeks)...
In vitro culture based approaches are time- and cost-effective solutions for rapidly evaluating the effects of drugs or natural compounds against microbiomes. The nutritional composition medium is an important determinant effectively maintaining gut microbiome in vitro. This study combines orthogonal experimental design a metaproteomics approach to obtaining functional insights into different components on microbiome. Our results show that metaproteomic profile respond differently...
ABSTRACT During host colonization, Campylobacter jejuni is exposed to harmful reactive oxygen species (ROS) produced from the immune system and gut microbiota. Consequently, identification characterization of oxidative stress defenses are important for understanding how C. survives ROS during colonization gastrointestinal tract. Previous transcriptomic studies have defined genes belonging oxidant stimulons within . We constructed isogenic deletion mutants these identified assess their role...